BibTex RIS Cite

Parinarik Asit Sıçan Torasik Aort Halkaları Endotel-Bağımlı Damar Gevşemesi Neden Olur

Year 2015, Volume: 1 Issue: 1, 43 - 49, 01.01.2015

Abstract

amaç: Çalışmanın amacı, nar çekirdeği yağı izole aorta halkalarında, vazodilatasyon sıçan oluşmasına neden olan olası mekanizmayı araştırmaktır.Gereç ve yöntemler: İzole sıçan arterler halkalar halinde kesilmiş ve Krebs çözümü ile dolu doku odalarına yerleştirildi. bulgular: PSO, endotel ile halkalarda konsantrasyona bağlı bir gevşeme üretti. Ancak, endotel olmadığında, herhangi bir gevşetici etkiye neden olmadı. L-NAME ve ODS, endotel ile halkalarda önemli oranda önlenmesini sağlar. L-arginin sonraki ekleme L-NAME önleyici etkilerini tersine çevirdi. Bununla birlikte, PSO'nun gevşetici etkisi ile ya da endotel olmayan ya da halkalarda, TEA ile 4-AP ve glibenklamid, değişmedi. Buna ek olarak, PSO'nun gevşetici etkisi indometasin, propranolol, losartan ya da kaptopril kuluçkalama ile etkilenmemiştir. Deneyler farelerin başka grup anestezi uygulandı ve cerrahi olarak, in vivo olarak kan basıncını kaydetmek üzere intraperitonal radyo vericisi ile donatılmıştır. PSO hafif birlikte sistolik ve diyastolik kan basıncında hafif bir düşüş kalp hızı azalmış neden. sonuç: Bu bulgular kapsamlı açıkça hipertansif hastalarda nitrik oksit-guanil siklaz yolunun modülasyonu yoluyla PSO tarafından uyarılan olduğunu göstermektedir

References

  • Jurenka JS. Therapeutic applications of pomegranate (Punica granatum L.): A review. Altern Med Rev 2008;13:128-44.
  • Lansky EP, Harrison G, Froom P, Jiang WG. Pomegranate (Punica granatum) pure chemicals show possible synergistic inhibition of human PC-3 prostate cancer cell invasion across Matrigel. Invest New Drugs 2005;23:121-2.
  • Fadavi A, Barzegar M, Azizi M. Determination of fatty acids and total lipid content in oilseed of 25 pomegranates varieties grown in Iran. Food Comp Anal 2006;19:676-80.
  • El-Shaarawy M, Nahapetian A. Studies on pomegranate seed oil. Fette Seife Anstrichmitte 1983;85:123-6.
  • Ozgul-Yucel S. Determination of conjugated linolenic acid content of selected oil seeds grown in Turkey. JAOCS 2005;82:893-7.
  • Ha YL, Grimm NK, Pariza MW. Anticarcinogens from fried ground beef: Heat-altered derivatives of linoleic acid. Carcinogenesis 1987;8:1881-7.
  • Tsuzuki T, Tokuyama Y, Igarashi M, Miyazawa T. Tumor growth suppression by alpha-eleostearic acid, a linolenic acid isomer with a conjugated triene system, via lipid peroxidation. Carcinogenesis 2004;25:1417-25.
  • Arao K, Yotsumoto H, Han SY, Nagao K, Yanagita T. The 9cis,11trans,13cis isomer of conjugated linolenic acid reduces apolipoprotein B100 secretion and triacylglycerol synthesis in HepG2 cells. Biosci Biotechnol Biochem 2004;68:2643-5.
  • Yamasaki M, Kitagawa T, Koyanagi N, Chujo H, Maeda H, Kohno-Murase J, et al. Dietary effect of pomegranate seed oil on immune function and lipid metabolism in mice. Nutrition 2006;22:54-9.
  • Sassano G, Sanderson P, Franx J, Groot P, Van Straalen J, Bassaganya-Riera J. Analysis of pomegranate seed oil for the presence of jacaric acid. J Sci Food Agric 2009;89:1046- 52.
  • Joh Y, Kim S, Christie W. The structure of the triacyglycerols, containing punicic acid, in the seed oil of Trichosanthes kirilowii. JAOCS 1995;72:1037-42.
  • Kaufman M, Wiesman z. Pomegranate oil analysis with emphasis on MALDI-TOF/MS triacylglycerol fingerprinting. J Agric Food Chem 2007;55:10405-13.
  • Ahlers N, Dennison A, O’Neill L. Spectroscopic examination of punicic acid. Nature 1954;173:1045-6.
  • Kohno H, Suzuki R, Yasui Y, Hosokawa M, Miyashita K, Tanaka T. Pomegranate seed oil rich in conjugated linolenic acid suppresses chemically induced colon carcinogenesis in rats. Cancer Sci 2004;95:481-6.
  • Hora JJ, Maydew ER, Lansky EP, Dwivedi C. Chemopre- ventive effects of pomegranate seed oil on skin tumor deve- lopment in CD1 mice. J Med Food 2003;6:157-61.
  • Albrecht M, Jiang W, Kumi-Diaka J, Lansky EP, Gommersall LM, Patel A, et al. Pomegranate extracts potently suppress proliferation, xenograft growth, and invasion of human prostate cancer cells. J Med Food 2004;7:274-83.
  • Lansky EP, Jiang W, Mo H, Bravo L, Froom P, Yu W, et al. Possible synergistic prostate cancer suppression by anatomically discrete pomegranate fractions. Invest New Drugs 2005;23:11-20.
  • Lansky EP, Newman RA. Punica granatum (pomegranate) and its potential for prevention and treatment of inflamma- tion and cancer. J Ethnopharmacol 2007;109:177-206.
  • Jeune MA, Kumi-Diaka J, Brown J. Anticancer activities of pomegranate extracts and genistein in human breast cancer cells. J Med Food 2005;8:469-75.
  • Arao K, Wang YM, Inoue N, Hirata J, Cha JY, Nagao K, et al. Dietary effect of pomegranate seed oil rich in 9cis, 11trans, 13cis conjugated linolenic acid on lipid metabolism in obese, hyperlipidemic OLETF rats. Lipids Health Dis 2004;3:24.
  • Vroegrijk IO, van Diepen JA, van den Berg S, Westbroek I, Keizer H, Gambelli L, et al. Pomegranate seed oil, a rich source of punicic acid, prevents diet-induced obesity and insulin resistance in mice. Food Chem Toxicol 2011;49:1426- 30.
  • McFarlin BK, Strohacker KA, Kueht ML. Pomegranate seed oil consumption during a period of high-fat feeding reduces weight gain and reduces type 2 diabetes risk in CD-1 mice. Br J Nutr 2009;102:54-9.
  • Aviram M, Rosenblat M, Gaitini D, Nitecki S, Hoffman A, Dornfeld L, et al. Pomegranate juice consumption for 3 years by patients with carotid artery stenosis reduces common carotid intima-media thickness, blood pressure and LDL oxidation. Clin Nutr 2004;23:423-33.
  • Standen NB, Quayle JM. K+ channel modulation in arterial smooth muscle. Acta Physiol Scand 1998;164:549-57.
  • Takahashi M, Yamashita A, Moriguchi-Goto S, Sugita C, Matsumoto T, Matsuda S, et al. Inhibition of factor XI reduces thrombus formation in rabbit jugular vein under endothelial denudation and/or blood stasis. Thromb Res 2010;125:464-70.
  • Mohan M, Waghulde H, Kasture S. Effect of pomegranate juice on Angiotensin II-induced hypertension in diabetic Wistar rats. Phytother Res 2010;24 Suppl 2:S196-203.
  • Mirmiran P, Fazeli MR, Asghari G, Shafiee A, Azizi F. Effect of pomegranate seed oil on hyperlipidaemic subjects: A double-blind placebo-controlled clinical trial. Br J Nutr 2010;104:402-6.
  • Vanhoutte PM. Endothelial dysfunction: The first step toward coronary arteriosclerosis. Circ J 2009;73:595-601.
  • Nelson MT, Quayle JM. Physiological roles and properties of potassium channels in arterial smooth muscle. Am J Physiol 1995;268:C799-822.

Punicic Acid Induces Endothelium-Dependent Vasorelaxation in Rat Thoracic Aortic Rings

Year 2015, Volume: 1 Issue: 1, 43 - 49, 01.01.2015

Abstract

Objective: The aim of the present study was to investigate the possible mechanism by which pomegranate seed oil causes vasodilatation in isolated thoracic aorta rings form the rat. Material and Methods: The isolated rat arteries were cut into rings and placed in tissue chambers filled with Krebs solution. results: Pomegranate seed oil produced a concentration-dependent relaxation in rings with endothelium. However, it didn’t cause any relaxant effect in the absence of endothelium. L-NAME and ODQ produced a significant inhibition in the rings with endothelium. Subsequent addition of L-arginine reversed the inhibitory effects of L-NAME. However, the relaxant effect of PSO did not change with tetraethylammonium, 4-aminopyridine and glibenclamide, in rings either with or without endothelium. Additionally, the relaxant effect of PSO was not affected by indomethacin, propranolol, losartan or captopril incubation. In another set of experiments rats were anaesthetised and surgically equipped with intraperitoneal radio-transmitters to record blood pressure in vivo. PSO caused a slight decrease in systolic and diastolic blood pressure along with mildly decreased heart rate. conclusion: These comprehensive findings clearly indicate that vasorelaxation is induced by PSO via modulation of nitric oxide-guanylyl cyclase pathway

References

  • Jurenka JS. Therapeutic applications of pomegranate (Punica granatum L.): A review. Altern Med Rev 2008;13:128-44.
  • Lansky EP, Harrison G, Froom P, Jiang WG. Pomegranate (Punica granatum) pure chemicals show possible synergistic inhibition of human PC-3 prostate cancer cell invasion across Matrigel. Invest New Drugs 2005;23:121-2.
  • Fadavi A, Barzegar M, Azizi M. Determination of fatty acids and total lipid content in oilseed of 25 pomegranates varieties grown in Iran. Food Comp Anal 2006;19:676-80.
  • El-Shaarawy M, Nahapetian A. Studies on pomegranate seed oil. Fette Seife Anstrichmitte 1983;85:123-6.
  • Ozgul-Yucel S. Determination of conjugated linolenic acid content of selected oil seeds grown in Turkey. JAOCS 2005;82:893-7.
  • Ha YL, Grimm NK, Pariza MW. Anticarcinogens from fried ground beef: Heat-altered derivatives of linoleic acid. Carcinogenesis 1987;8:1881-7.
  • Tsuzuki T, Tokuyama Y, Igarashi M, Miyazawa T. Tumor growth suppression by alpha-eleostearic acid, a linolenic acid isomer with a conjugated triene system, via lipid peroxidation. Carcinogenesis 2004;25:1417-25.
  • Arao K, Yotsumoto H, Han SY, Nagao K, Yanagita T. The 9cis,11trans,13cis isomer of conjugated linolenic acid reduces apolipoprotein B100 secretion and triacylglycerol synthesis in HepG2 cells. Biosci Biotechnol Biochem 2004;68:2643-5.
  • Yamasaki M, Kitagawa T, Koyanagi N, Chujo H, Maeda H, Kohno-Murase J, et al. Dietary effect of pomegranate seed oil on immune function and lipid metabolism in mice. Nutrition 2006;22:54-9.
  • Sassano G, Sanderson P, Franx J, Groot P, Van Straalen J, Bassaganya-Riera J. Analysis of pomegranate seed oil for the presence of jacaric acid. J Sci Food Agric 2009;89:1046- 52.
  • Joh Y, Kim S, Christie W. The structure of the triacyglycerols, containing punicic acid, in the seed oil of Trichosanthes kirilowii. JAOCS 1995;72:1037-42.
  • Kaufman M, Wiesman z. Pomegranate oil analysis with emphasis on MALDI-TOF/MS triacylglycerol fingerprinting. J Agric Food Chem 2007;55:10405-13.
  • Ahlers N, Dennison A, O’Neill L. Spectroscopic examination of punicic acid. Nature 1954;173:1045-6.
  • Kohno H, Suzuki R, Yasui Y, Hosokawa M, Miyashita K, Tanaka T. Pomegranate seed oil rich in conjugated linolenic acid suppresses chemically induced colon carcinogenesis in rats. Cancer Sci 2004;95:481-6.
  • Hora JJ, Maydew ER, Lansky EP, Dwivedi C. Chemopre- ventive effects of pomegranate seed oil on skin tumor deve- lopment in CD1 mice. J Med Food 2003;6:157-61.
  • Albrecht M, Jiang W, Kumi-Diaka J, Lansky EP, Gommersall LM, Patel A, et al. Pomegranate extracts potently suppress proliferation, xenograft growth, and invasion of human prostate cancer cells. J Med Food 2004;7:274-83.
  • Lansky EP, Jiang W, Mo H, Bravo L, Froom P, Yu W, et al. Possible synergistic prostate cancer suppression by anatomically discrete pomegranate fractions. Invest New Drugs 2005;23:11-20.
  • Lansky EP, Newman RA. Punica granatum (pomegranate) and its potential for prevention and treatment of inflamma- tion and cancer. J Ethnopharmacol 2007;109:177-206.
  • Jeune MA, Kumi-Diaka J, Brown J. Anticancer activities of pomegranate extracts and genistein in human breast cancer cells. J Med Food 2005;8:469-75.
  • Arao K, Wang YM, Inoue N, Hirata J, Cha JY, Nagao K, et al. Dietary effect of pomegranate seed oil rich in 9cis, 11trans, 13cis conjugated linolenic acid on lipid metabolism in obese, hyperlipidemic OLETF rats. Lipids Health Dis 2004;3:24.
  • Vroegrijk IO, van Diepen JA, van den Berg S, Westbroek I, Keizer H, Gambelli L, et al. Pomegranate seed oil, a rich source of punicic acid, prevents diet-induced obesity and insulin resistance in mice. Food Chem Toxicol 2011;49:1426- 30.
  • McFarlin BK, Strohacker KA, Kueht ML. Pomegranate seed oil consumption during a period of high-fat feeding reduces weight gain and reduces type 2 diabetes risk in CD-1 mice. Br J Nutr 2009;102:54-9.
  • Aviram M, Rosenblat M, Gaitini D, Nitecki S, Hoffman A, Dornfeld L, et al. Pomegranate juice consumption for 3 years by patients with carotid artery stenosis reduces common carotid intima-media thickness, blood pressure and LDL oxidation. Clin Nutr 2004;23:423-33.
  • Standen NB, Quayle JM. K+ channel modulation in arterial smooth muscle. Acta Physiol Scand 1998;164:549-57.
  • Takahashi M, Yamashita A, Moriguchi-Goto S, Sugita C, Matsumoto T, Matsuda S, et al. Inhibition of factor XI reduces thrombus formation in rabbit jugular vein under endothelial denudation and/or blood stasis. Thromb Res 2010;125:464-70.
  • Mohan M, Waghulde H, Kasture S. Effect of pomegranate juice on Angiotensin II-induced hypertension in diabetic Wistar rats. Phytother Res 2010;24 Suppl 2:S196-203.
  • Mirmiran P, Fazeli MR, Asghari G, Shafiee A, Azizi F. Effect of pomegranate seed oil on hyperlipidaemic subjects: A double-blind placebo-controlled clinical trial. Br J Nutr 2010;104:402-6.
  • Vanhoutte PM. Endothelial dysfunction: The first step toward coronary arteriosclerosis. Circ J 2009;73:595-601.
  • Nelson MT, Quayle JM. Physiological roles and properties of potassium channels in arterial smooth muscle. Am J Physiol 1995;268:C799-822.
There are 29 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Bedriniam Yılmaz This is me

Coskun Usta This is me

Arda Taşatargil This is me

Semir Özdemir This is me

Publication Date January 1, 2015
Published in Issue Year 2015 Volume: 1 Issue: 1

Cite

Vancouver Yılmaz B, Usta C, Taşatargil A, Özdemir S. Parinarik Asit Sıçan Torasik Aort Halkaları Endotel-Bağımlı Damar Gevşemesi Neden Olur. Akd Med J. 2015;1(1):43-9.