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Bazı Allium Türlerinin RAPD-PCR ile Genetik Analizleri

Year 1997, Volume: 10 Issue: 1, 136 - 142, 01.12.1997

Leyla Açık Bülent Samancı Mehmet Yapar Ayhan Kubar

Abstract

RAPD belirleyicileri Türkiye'de bulunan Allium türlerini ayırmak ve belirlemek için kullanıldı: A. neopolitanum, A. callidictyon, A. rupestre, A. scorodoprasıun, A. affine ve A. junceum. A10 primeri faydalılığı için değerlendirildi. A10 primeri ile reaksiyonlar amplifike olmuş band üretti ve amplifike olmuş DNA parçalarının büyüklüğü 100-947 baz çifti arasında değişti. Diğer 11 primer grubu ile reaksiyonlar bazı türlerin ayrışımını kolaylaştırdı. A. neopolitanum, A. callidictyon, A. rupestre, A. scorodoprasum, A. affine ve A. junceum arasındaki genetik uzaklıklar hesaplandı.

Keywords

RAPD-PCR Genetik Analiz Dendogram

References

  • 1. AÇIK L., B. SAMANCI, F. DUMAN, F. UNAL. Polymorphism and Phylogenetic Relations among Turkish Species in The Genus Allium as Determined RAPD-PCR. Turkish Journal of Botany. In press. 1997.
  • 2. BADR, A., T. ELKINGTON. Numeral Taxonomy of Species in Allium New Phytol, 81, 401-364, 1993.
  • 3. DAVIS, P. H. In Flora of Turkey and East Aegean Islands. Vol VIII. pp.360-364, 1984,
  • 4. HADOCOVA,V.,J.SVACHULOVA E. KLOZOVA, HADOC, K. PITTEROVA. Use of Estrase Isoenzymes Revealed by Gel Isoelectric Focusing as and Aid in Chemataxonomical Study of the Genus Allium. Bio Plant, Praque. 259,36-42, 1983.
  • 5. FIANELT, P. In Onions and Allied Crops VI. II. Botany, Physiology and Genetics. CRC Press Inc. Boca, Raton, Florida, pp.1-26, 1989.
  • 6. RAFALSKI, I.A., S.V. TINGEY, J.G.K. WILLIAMS. RAPD Markers- a New Technology' for Genetic Mapping and Plant Breeding. Ag. Biotech News Info.3, 645-648, 1991
  • 7. SAMBROOK, J„ F.F. FRITCH, T. MANIATIS. In Molecular Clonning. A Laboratory Manual. Second edition, Cold Spring Harbour, 1989.
  • 8. STEARN, W. Notes on the Genus Allium in the Old World. Herbertia. 11, 11-30.1944.
  • 9. TANKSLEY, S.D., N.D. YOUNG, A.H. PETERSON, M.W. NONIERBALE. RFLP Mapping in Plant Breeding New Tools for an Old Science. Biotechnolgy, 7:257-264, 1989.
  • 10. WELSH, J., C. PETERSON, M. MCCLLELAND. Polymorphism Generated by Arbitrarily Primed PCR in the Mouse: Applications to Strain Identification and Genetic Mapping, Nucleic Acids Res. 19, 903-906, 1991.
  • 11. WELSH, 1, C. PRETZMEN, D. POSTIC, T. SAINT-GRIONS, G. BATONTON, M. MCCLELLAND. Genomic Fingerprinting br Arbitrarily Primed PCR Resolves Borrelia burgdorferi into Three Distinct Phyletic Groups. Int. Sys. Bactenol. 42, 370-377, 1992.
  • 12. WILKIE, S.E., P G. ISAAC, J.R. SLATER. RAPD Markers for Genetic Analysis in Alihan. Thor Appl. Genet. 86, 497-504, 1993.
  • 13. WILLIAMS J.G., A.R. KUBELIK, K, LLIVAC, J. A. RAFALSKI, S. TINGEY. DNA polymporphism Amplified by Arbitrary Primers are Useful as Genetic Markers. Nucleic Acids Research. 18 (22), 6531-6535, 1990.
  • 14. WOLF, K., J.P. RIJN. Rapid Detection of Genteic Variablity in Chrisanthemun Using Random Primers. Heridity. 71,335-341, 1993.

Genetic Analysis of Certain Allium Species with RAPD-PCR

Year 1997, Volume: 10 Issue: 1, 136 - 142, 01.12.1997

Leyla Açık Bülent Samancı Mehmet Yapar Ayhan Kubar

Abstract

Ran dom amplified polymorphic DNA (RAPD) markers were used to identify and distinguish between several Turkish Allium species: A. neopolitanum, A. callidictyon, A. rupestre, A. scorodoprasımı, A. affine and A. junceum. The primers A10 were evaluated for their usefulness. Reactions with primer A 10 produced amplified band and the size of amplified DNA fragments ranged from 100 to 947 base pairs. Reactions with another group of 11 primers made possible the separation of some but not all species. Genetic distances between A. neopolitanum, A. callidictyon, A. rupestre, A. scorodopr asam, A. affine and A. junceum were calculated.

Keywords

RAPD-PCR Genetic Analysis Dendogram

References

  • 1. AÇIK L., B. SAMANCI, F. DUMAN, F. UNAL. Polymorphism and Phylogenetic Relations among Turkish Species in The Genus Allium as Determined RAPD-PCR. Turkish Journal of Botany. In press. 1997.
  • 2. BADR, A., T. ELKINGTON. Numeral Taxonomy of Species in Allium New Phytol, 81, 401-364, 1993.
  • 3. DAVIS, P. H. In Flora of Turkey and East Aegean Islands. Vol VIII. pp.360-364, 1984,
  • 4. HADOCOVA,V.,J.SVACHULOVA E. KLOZOVA, HADOC, K. PITTEROVA. Use of Estrase Isoenzymes Revealed by Gel Isoelectric Focusing as and Aid in Chemataxonomical Study of the Genus Allium. Bio Plant, Praque. 259,36-42, 1983.
  • 5. FIANELT, P. In Onions and Allied Crops VI. II. Botany, Physiology and Genetics. CRC Press Inc. Boca, Raton, Florida, pp.1-26, 1989.
  • 6. RAFALSKI, I.A., S.V. TINGEY, J.G.K. WILLIAMS. RAPD Markers- a New Technology' for Genetic Mapping and Plant Breeding. Ag. Biotech News Info.3, 645-648, 1991
  • 7. SAMBROOK, J„ F.F. FRITCH, T. MANIATIS. In Molecular Clonning. A Laboratory Manual. Second edition, Cold Spring Harbour, 1989.
  • 8. STEARN, W. Notes on the Genus Allium in the Old World. Herbertia. 11, 11-30.1944.
  • 9. TANKSLEY, S.D., N.D. YOUNG, A.H. PETERSON, M.W. NONIERBALE. RFLP Mapping in Plant Breeding New Tools for an Old Science. Biotechnolgy, 7:257-264, 1989.
  • 10. WELSH, J., C. PETERSON, M. MCCLLELAND. Polymorphism Generated by Arbitrarily Primed PCR in the Mouse: Applications to Strain Identification and Genetic Mapping, Nucleic Acids Res. 19, 903-906, 1991.
  • 11. WELSH, 1, C. PRETZMEN, D. POSTIC, T. SAINT-GRIONS, G. BATONTON, M. MCCLELLAND. Genomic Fingerprinting br Arbitrarily Primed PCR Resolves Borrelia burgdorferi into Three Distinct Phyletic Groups. Int. Sys. Bactenol. 42, 370-377, 1992.
  • 12. WILKIE, S.E., P G. ISAAC, J.R. SLATER. RAPD Markers for Genetic Analysis in Alihan. Thor Appl. Genet. 86, 497-504, 1993.
  • 13. WILLIAMS J.G., A.R. KUBELIK, K, LLIVAC, J. A. RAFALSKI, S. TINGEY. DNA polymporphism Amplified by Arbitrary Primers are Useful as Genetic Markers. Nucleic Acids Research. 18 (22), 6531-6535, 1990.
  • 14. WOLF, K., J.P. RIJN. Rapid Detection of Genteic Variablity in Chrisanthemun Using Random Primers. Heridity. 71,335-341, 1993.
There are 14 citations in total.

Details

Primary Language English
Subjects Agricultural Engineering
Journal Section Articles
Authors

Leyla Açık This is me

Bülent Samancı This is me

Mehmet Yapar This is me

Ayhan Kubar This is me

Publication Date December 1, 1997
Published in Issue Year 1997 Volume: 10 Issue: 1

Cite

APA Açık, L., Samancı, B., Yapar, M., Kubar, A. (1997). Genetic Analysis of Certain Allium Species with RAPD-PCR. Akdeniz University Journal of the Faculty of Agriculture, 10(1), 136-142.
AMA Açık L, Samancı B, Yapar M, Kubar A. Genetic Analysis of Certain Allium Species with RAPD-PCR. Akdeniz University Journal of the Faculty of Agriculture. December 1997;10(1):136-142.
Chicago Açık, Leyla, Bülent Samancı, Mehmet Yapar, and Ayhan Kubar. “Genetic Analysis of Certain Allium Species With RAPD-PCR”. Akdeniz University Journal of the Faculty of Agriculture 10, no. 1 (December 1997): 136-42.
EndNote Açık L, Samancı B, Yapar M, Kubar A (December 1, 1997) Genetic Analysis of Certain Allium Species with RAPD-PCR. Akdeniz University Journal of the Faculty of Agriculture 10 1 136–142.
IEEE L. Açık, B. Samancı, M. Yapar, and A. Kubar, “Genetic Analysis of Certain Allium Species with RAPD-PCR”, Akdeniz University Journal of the Faculty of Agriculture, vol. 10, no. 1, pp. 136–142, 1997.
ISNAD Açık, Leyla et al. “Genetic Analysis of Certain Allium Species With RAPD-PCR”. Akdeniz University Journal of the Faculty of Agriculture 10/1 (December 1997), 136-142.
JAMA Açık L, Samancı B, Yapar M, Kubar A. Genetic Analysis of Certain Allium Species with RAPD-PCR. Akdeniz University Journal of the Faculty of Agriculture. 1997;10:136–142.
MLA Açık, Leyla et al. “Genetic Analysis of Certain Allium Species With RAPD-PCR”. Akdeniz University Journal of the Faculty of Agriculture, vol. 10, no. 1, 1997, pp. 136-42.
Vancouver Açık L, Samancı B, Yapar M, Kubar A. Genetic Analysis of Certain Allium Species with RAPD-PCR. Akdeniz University Journal of the Faculty of Agriculture. 1997;10(1):136-42.