Geri Döngü Akışlı Ultrasonik Reaktörde Ag+ ile Escherichia coli Dezenfeksiyonu

Year 2022, , 805 - 813, 31.08.2022

Fadime Karaer Özmen

https://doi.org/10.35414/akufemubid.1125089

Abstract

Son zamanlarda yaşanan pandemi sürecinin ardından bulaşıcı hastalıkların kontrolü için kullanılan dezenfeksiyon/sterilizasyon yöntemleri daha da önem kazanmıştır. Patojenik mikroorganizmaların neden olduğu salgın hastalıkların önlenmesinde sıklıkla kullanılan klor uygulamaları, ozon ve ultraviyole gibi mikrobiyal inaktivasyon süreçlerinin sahip olduğu olumsuz etkiler nedeniyle ultrases (US) gibi yeni dezenfeksiyon yöntemleri geliştirilmektedir. Bu çalışma kapsamında 22kHz, 36kHz ve 833kHz ultrasonik frekanslarda çalıştırılan geri döngülü akışlı ultrasonik reaktörlerde su kaynaklarında fekal kontaminasyon varlığını işaret eden Escherichia coli bakterisi kullanılarak dezenfeksiyon işlemleri gerçekleştirilmiştir. Bu çalışmada esas olarak, gümüş iyonlarının (Ag+) (0.1 mM, 0.01 mM ve 0.005 mM Ag+) US ile (22 kHz, 36 kHz ve 833 kHz ultrasonik frekanslar) hibrit kullanımı 1x104 CFU/mL başlangıç Escherichia coli bakteri konsantrasyonu kullanılarak araştırılmıştır. Çalışma sonucunda Escherichia coli minimum Ag+ konsantrasyonunda ve 22 kHz ultrasonik frekansta 4-log giderim verimi elde edilerek inaktive edilmiştir. Sonuç olarak, US ve Ag+ yöntemleriyle dezenfeksiyon etkili bir şekilde sağlanmış ve US-Ag+ ikili proseslerinde, sinerjik etkileri nedeniyle daha hızlı dekontaminasyon süresi ve daha düşük Ag+ konsantrasyonu ile daha fazla Escherichia coli inaktivasyon oranı elde edilmiştir.

Keywords

Dezenfeksiyon, Escherichia coli, Ultrases, Gümüş iyonu, Geri döngülü akış

Escherichia coli Disinfection with Ag+ in the Recycled Flow Ultrasonic Reactor

Abstract

After the recent pandemic process, disinfection/sterilization methods used for the control of infectious diseases have gained even more importance. The alternative disinfection studies like ultrasound (US) have been developing due to the various shortcomings of microbial inactivation processes such as chlorine application, ozone and ultraviolet radiation, which are widely applied for the prevention of epidemic diseases caused by pathogenic microorganisms. Within the scope of this study, disinfection treatments were carried out in recycled flow ultrasonic reactors operated at 22kHz, 36kHz and 833kHz ultrasonic frequencies using Escherichia coli bacteria indicated fecal contamination in the water sources. In this article, the combined usage of silver ions (0.1mM, 0.01 mM and 0.005 mM Ag+) and US (22 kHz, 36 kHz and 833 kHz ultrasonic frequencies) were mainly investigated using initial Escherichia coli bacteria concentration of 1x104 CFU/mL. As a result of the study, Escherichia coli was inactivated obtaining 4-log reduction with 22 kHz ultrasonic frequency with the minimum Ag+ concentrations. To conclude, the disinfection was effectively achieved with US and Ag+ methods, and higher Escherichia coli inactivation rate were obtained in US-Ag+ dual processes with faster decontamination time and lower Ag+ concentration due to their synergistic effects.

Keywords

Disinfection, Escherichia coli, Ultrasound, Silver ion, Recycled flow

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