The Role of Chitosan, a Potential Organic Substance, in Combating Salt Stress in Tomato

Year 2023, , 1085 - 1093, 30.10.2023

Hüseyin Bulut , Halil İbrahim Öztürk

https://doi.org/10.35414/akufemubid.1287609

Abstract

Soil salinity is one of the critical factors that cause yield losses in tomato cultivation. Dealing with stress
caused by salinity has recently become a global issue. It is known that chitosan has a role in reducing
the harmful effects of abiotic stresses on plants. Chitosan, which is used as a non-toxic, biocompatible,
biodegradable, and organic supplement, has attracted great interest in agricultural production in recent
years. In this study, the effect of chitosan on morphological growth parameters and its role in healing
seed damage in tomato seedlings under salt stress was investigated. The extent of salt damage in
tomato seedlings and the effect of chitosan against it was evaluated by morphological parameters and
DNA damage by Comet assay. The morphological effect of salt stress on seedlings was obtained from
plant height, stem height, stem diameter, leaf area, and petiole length data. The level of DNA damage
caused by stress was determined by the tail length, tail DNA % value, and tail moment, which are
considered as indicators of DNA damage. It was determined that the applied chitosan had a positive
effect on the morphological characteristics of tomato seedlings. It was determined that chitosan
supplementation was effective in reducing DNA damage at some doses. Our study results determined
that the applied salt caused stress in tomato seedlings depending on the dose and chitosan could be
used effectively to protect tomato seedlings from the destructive effects of salt stress. It can also be
used to detect DNA damage in future studies with the comet assay technique.

Keywords

chitosan, Comet assay, Salinity stress, Tomato

Project Number

FBA-2021-765

Domateste Tuz Stresi İle Mücadelede Potansiyel Bir Organik Madde Olan Kitosanın Rolü

Abstract

Domates yetiştiriciliğinde verim kayıplarına neden olan kritik faktörlerden birisi toprak tuzluluğudur.
Tuzluluğun neden olduğu stres ile mücadele son zamanlarda küresel bir konu haline gelmiştir. Kitosanın
abiyotik streslerin bitkiler üzerinde neden olduğu zararlı etkilerini azaltmada rolü olduğu bilinmektedir.
Toksik olmayan, biyolojik olarak uyumlu, biyolojik olarak parçalanabilen ve organik bir takviye olarak
kullanılan kitosan, tarımsal üretimde son yıllarda büyük ilgi görmektedir. Bu çalışmada, tuz stresi
altındaki domates fidelerinde kitosanın morfolojik büyüme parametrelerine etkisi ve çekirdek hasarını
iyileştirmedeki rolü incelenmiştir. Domates fidelerinde tuz hasarının boyutu ve kitosanın buna karşı
etkisi morfolojik parametreler ve Comet assay ile DNA hasarı değerlendirilmiştir. Tuz stresinin fideler
üzerindeki morfolojik etkisi bitki boyu, gövde yüksekliği, gövde çapı, yaprak alanı ve yaprak sapı
uzunluğu verilerinden elde edilmiştir. Stres etkisiyle oluşan DNA hasar düzeyi, DNA hasarının göstergesi
olarak kabul edilen kuyruk uzunluğu, kuyruk DNA % değeri ve kuyruk momenti ile belirlenmiştir.
Uygulanan kitosanın domates fidelerinin morfolojik özellikleri üzerinde olumlu etkisi olduğu
saptanmıştır. Kitosan takviyesinin bazı dozlarda DNA hasarını azaltmada etkili olmuştur. Çalışma
sonuçlarımız uygulanan tuzun domates fidelerinde doz miktarına bağlı olarak strese neden olduğunu ve
kitosanın domates fidelerini tuz stresinin yıkıcı etkilerinden korumak için etkin bir şekilde
kullanılabileceğini ortaya koymuştur.

Keywords

Comet assay, Domates, Kitosan, Tuzluluk stresi

Supporting Institution

Erzincan Binali Yıldırım Üniversitesi BAP Koordinatörlüğü

Project Number

FBA-2021-765

Thanks

Bu çalışma Erzincan Binali Yıldırım Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü (BAP) tarafından desteklenen FBA-2021-765 kodlu projeden elde edilmiştir. Katkıları için teşekkür ederiz.

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