Chemical Components and Antibacterial Activity of Asphalt Grass (Bituminaria bituminosa (L.) C.H.Stirt) Growing in Tekirdag Province

Year 2024, , 773 - 781, 20.08.2024

Hilmican Çalışkan , Muazzez Gürgan Eser

https://doi.org/10.35414/akufemubid.1417456

Abstract

The aim of this study was to investigate the chemical constituents of Bituminaria bituminosa (L.) C.H.Stirt growing in Tekirdag province by GC-MS method and to determine the antibacterial activity of crude extracts. The chemical constituents of hexane, dichloromethane and methanol extracts obtained from B. bituminosa plant by Soxhlet method were investigated using GC-MS method. As a result of our research on the chemical components of B. bituminosa, thirty-four compounds were detected in hexane extract, thirty-eight compounds in dichloromethane extract and thirty-five compounds in methanol extract. The highest antibacterial effect on both Gram-positive and Gram-negative bacteria tested in the study was obtained in the hexane extract of B. bituminosa.

Keywords

Bituminaria, B. bituminosa, Chemical Components, GC-MS Analysis, Antibacterial Activity

Tekirdağ İlinde Yetişen Asfalt Otu (Bituminaria bituminosa (L.) C.H.Stirt) Bitkisinin Kimyasal Bileşenleri ve Antibakteriyel Aktivitesi

Abstract

Bu çalışmadaki amacımız, Tekirdağ ilinde yetişen Bituminaria bituminosa (L.) C.H.Stirt bitkisinin kimyasal bileşenlerini GC-MS yöntemi ile araştırmak ve ham ekstrelerinde antibakteriyel aktivitesini belirlemektir. B. bituminosa bitkisinden Soxhlet metodu ile elde edilen hekzan, diklorometan ve metanol ekstresinin kimyasal bileşenleri, GC-MS yöntemi kullanılarak araştırılmıştır. B. bituminosa'nın kimyasal bileşenleri üzerine yaptığımız araştırma sonucunda hekzan ekstresinde otuz dört adet, diklorometan ekstresinde otuz sekiz adet ve metanol ekstresinde otuz beş adet bileşik tespit edilmiştir. Çalışmada denenen hem Gram-pozitif hem de Gram-negatif bakteriler üzerinde en yüksek antibakteriyel etki B. bituminosa'nın hekzan ekstresinde elde edilmiştir.

Keywords

Bituminaria, B. bituminosa, Kimyasal Bileşen, GC-MS Analizi, Antibakteriyel Aktivite

References

• Abascal, K. and Yarnell, E., 2002. Herbs and Drug Resistance: Potential of Botanical in Drug Resistant Microbes. Alternative & Complementary Therapies, 1, 237-241. https://doi.org/10.1089/107628002320351370
• Al Ayoubi, S., Raafat, K., El-Lakany, A. and Aboul-Ela, M., 2018. Phytochemical investigation of Psoralea bituminosa L. and its anti-diabetic potentials. Pharmacognosy Journal, 10(5), 841-853. https://doi.org/10.5530/pj.2018.5.143
• Alma, M. and Ertas, M., 2007. Essential oil content, Turkish Clove. Bio Resources, 2(2), 265-269.
• Andrade, B.F.M.T., Barbosa, L.N., Probst I.S. and Junior, A.F., 2014. Antimicrobial activity of essential oils. Journal of Essential Oil Research, 26, 34-40. https://doi.org/10.1080/10412905.2014.926087
• Azzouzi, S., Zaabat, N., Medjroubi, K., Akkal, S., Benlabed, K., Smati, F. and Dijoux-Franca, M.G., 2014. Phytochemical and biological activities of Bituminaria bituminosa L. (Fabaceae). Asian Pacific Journal of Tropical Medicine, 7(1), 481-484. https://doi.org/10.1016/S1995-7645(14)60278-9
• Begum, I.F., Mohankumar, R., Jeevan, M. and Ramani, K., 2016. GC-MS analysis of bio-active molecules derived from Paracoccus pantotrophus FMR19 and the antimicrobial activity against bacterial pathogens and MDROs. Indian Journal of Microbiology, 56, 426-432. https://doi.org/10.1007/s12088-016-0609-1
• Balouiri, M., Sadiki, M. and Ibnsouda, S.K., 2016. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71-79. https://doi.org/10.1016/j.jpha.2015.11.005
• Berber, İ., Avşar, C., Çine, N., Bozkurt, N. and Elmas, E., 2013. Sinop’da yetişen bazı bitkilerin metanolik ekstraklarının antibakteriyel ve antifungal aktivitelerinin belirlenmesi. Karaelmas Fen ve Mühendislik Dergisi, 3, 10-16. https://doi.org/10.7212/zkufbd.v3i1.87
• Cos, P., Vlietinck, A.J., Berghe, D.V. and Maes, L. 2006. Anti-infective potential of natural products: how to develop a stronger in vitro “proof-of-concept”. Journal of Ethnopharmacology, 106(3), 290-302. https://doi.org/10.1016/j.jep.2006.04.003
• Darcan, C., Kaygusuz, Ö., Leblebici, S., Gülümser, E. and Acar, Z., 2021. Antimicrobial activity of leaf extracts of Bituminaria sp. genotypes at different growth stages. Indian Journal of Experimental Biology, 59, 302-309. https://doi.org/10.56042/ijeb.v59i05.50484
• Davis, P.H., 1965. Flora of Turkey and the East Aegean Islands. Edinburgh University Press, Edinburgh, 567.
• Dorman, H.J.D. and Deans, S.G., 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology, 88, 308-316. https://doi.org/10.1046/j.1365-2672.2000.00969.x
• Erdoğan, A.E. and Everest, A., 2013. Antimikrobiyal ajan olarak bitki bileşenleri. Türk Bilimsel Derlemeler Dergisi, 6, 27-32.
• Fitzgerald, K.T. and Newquist, K.L., 2013. Poisonings in the Captive Reptile. In Small Animal Toxicology, W.B. Saunders, 229-249. https://doi.org/10.1016/B978-1-4557-0717-1.00020-X
• Gülbandılar, A., 2009. Investigation of Staphylococcus aureus carriage and antibiotic sensitivity in nose mucous membrane in Kutahya Province. DPÜ Fen Bilimleri Dergisi, 18, 1-6.
• Gutman, M., Perevolotsky, A. and Sternberg, M., 2000. Grazing effects on a perennial legume, Bituminaria bituminosa (L.) Stirton, in a Mediterranean rangeland. Cahiers Options Mediterraneennes, 45, 299-303.
• Houghton, R.A., Skole, D.L., Nobre, C.A., Hackler, J.L., Lawrence, K.T. and Chomentowski, W.H., 2000. Annual fluxes of carbon from deforestation and regrowth in the Brazilian Amazon. Nature, 403, 301-304. https://doi.org/10.1038/35002062
• Jang, J., Hur, H.G., Sadowsky, M.J., Byappanahalli, M.N., Yan, T. and Ishii, S., 2017. Environmental Escherichia coli: ecology and public health implications-a review. Journal of Applied Microbiology, 123(3), 570-581. https://doi.org/10.1111/jam.13468
• Kabelitz, N., Santos, P.M., Heipieper, H.J., 2003. Effect of aliphatic alcohols on growth and degree of saturation of membrane lipids in Acinetobacter calcoaceticus. FEMS Microbiology Letters, 220, 223-227. https://doi.org/10.1016/S0378-1097(03)00103-4
• Karasu, K. and Öztürk, E., 2014. Tıbbi ve aromatik bitkilerin kanatlılarda antioksidan ve antimikrobiyal etkileri, Türk Tarim ve Doğa Bilimleri Dergisi, 2, 1766-1772.
• Kasuni Keshala, K., Bandara, A.M.P.W., Padumadasa, C. and Peiris, L.D.C., 2021. Bioactivities and GC-MS profiling of Malewana Madhumeha Choorna polyherbal hot infusion. South African Journal of Botany, 140, 194-203. https://doi.org/10.1016/j.sajb.2021.04.015
• Lansford, E.M., Jr., Hill, I.D., Shive, W., 1960. Reversal by ribonucleosides of bacterial growth inhibition caused by alcohol. Journal of Biological Chemistry, 235, 3551-3555. https://doi.org/10.1016/S0021-9258(18)64506-5
• Laxminarayan, R., Duse, A., Wattal, C., Zaidi, A.K.M., Wertheim, H.F.L., Sumpradit, N., Vlieghe, E., Hara, G.L., Gould, I.M., Goossens, H., Greko, C., So, A.D., Bigdeli, M., Tomson, G., Woodhouse, W., Ombaka, E., Peralta, A.Q., Qamar, F.N., Mir, F., Kariuki, S., Bhutta, Z.A., Coates, A., Bergstrom, R., Wright, G.D., Brown, E.D, Cars, O., 2013. Antibiotic resistance-the need for global solutions. The Lancet Infectious Diseases, 13(12), 1057-1098. https://doi.org/10.1016/S1473-3099(13)70318-9
• Lemouchi, R., Selles, C., El Amine Dib, M., Benmansour, N., Allal, A., Tabti, B. and Ouali, K., 2017. Chemical composition and antioxidant avtivity of essential oil and hydrosol extract obtained by hydrodistillation (HY) and liquid-liquid extraction (LLE) of Psoralea bituminosa. Journal of Herbs, Spices and Medicinal Plants, 23(4), 299-307. https://doi.org/10.1080/10496475.2017.1329765
• Machowska, A. and Stalsby Lundborg, 2018. Drivers of Irrational Use of Antibiotics in Europe. International Journal of Environmental Research and Public Health, 16(1), 1-14. https://doi.org/10.3390/ijerph16010027
• Mates, A., 1974. The effect of alcohols on growth and lipase formation by Staphylococcus aureus. Journal of Applied Bacteriology, 37, 1-6. https://doi.org/10.1111/j.1365-2672.1974.tb00409.x
• Munoz, A. and Correal, E., 1998. Bituminaria bituminosa, leguminosa de interes forajero en la Cuenca mediterranea: I. Situacion taxonomica, autoecologica y distribución. Actos de la XXXVIII Reunión Cientifica de la S.E.E.P. Soria, 87-91.
• Naeim, H., El-Hawiet, A., Abdel Rahman R.A., Hussein, A., El Demellawy, M.A. and Embaby, A.M, 2020. Antibacterial activity of Centaurea pumilio L. root and aerial part extracts against some multidrug resistant bacteria. BMC Complementary Medicine and Therapies, 20, 1-13. https://doi.org/10.1186/s12906-020-2876-y
• Ngo, T.V., Scarlett, C.J., Bowyer, M.C., Ngo, P.D. and Vuong, Q.V., 2017. Impact of different extraction solvents on bioactive compounds and antioxidant capacity from the root of Salacia chinensis L. Journal of Food Quality, 2017, 1-8. https://doi.org/10.1155/2017/9305047
• Oğuztürk, G.E., Turna, T., Yüksek, T. and Uğur Kaval, U., 2021. Bazı Bitki Ekstraktlarının Antiviral Etkileri Üzerine Bir Derleme. Bayburt Üniversitesi Fen Bilimleri Dergisi, 4(2), 156-163.
• Orak, H.H., Sevik Bahrisefit I. and Sabudak, T., 2019. Antioxidant Activity of Extracts of Soursop (Annona muricata L.) Leaves, Fruit Pulps, Peels, and Seeds. Polish Journal of Food and Nutrition Sciences, 69(4), 359-366. https://doi.org/10.31883/pjfns/112654
• Rao, S.V., Tulasi, D.P., Pavithra, K., Rameen, T.S. and Balasubramanian, S., 2018. In silico studies on dengue and MERS coronavirus proteins with selected Coriandrum sativum L. herb constituents. World Journal of Pharmaceutical Sciences, 7(12), 970-989. https://doi.org/10.20959/wjpps201812-12821
• Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K.M. and Latha L.Y., 2011. Extraction, Isolation and Characterization of Bioactive Compounds from Plants’ Extracts. African Journal of Traditional, Complementary and Alternative Medicines, 8(1), 1-10. https://doi.org/10.4314/ajtcam.v8i1.60483
• Satıl, F., Azcan, N. and Baser, K.H.C., 2003. Fatty acid composition of Pistachio nuts in Turkey. Chemistry of Natural Compounds, 39, 322-324. https://doi.org/10.1023/B:CONC.0000003408.63300.b5
• Şahin, B., 2013. Farklı ekim zamanlarında yetiştirilen bazi tibbi bitkilerin verim ve kalite özelliklerinin belirlenmesi. Yüksek Lisans Tezi, Fen Bilimleri Enstitüsü, Selçuk Üniversitesi, Konya. 143.
• Usai, M., Marchetti, M., Melis, R.A.M. and Porqueddu, C., 2021. Volatolomics of Sarinian and Spanish Bituminaria: Characterization of different accessions using chemometrics. Molecules, 26, 1-28. https://doi.org/10.3390/molecules26175247
• Vanitha, V., Vijayakumar, S., Nilavukkarasi, M., Punitha, V.N., Vidhya, E. and Praseerha, P.K., 2020. Heneicosane-A novel microbicidal bioactive alkane identified from Plumbago zeylanica L. Industrial Crops and Products, 154, 112748. https://doi.org/10.1016/j.indcrop.2020.112748
• Ventura, M.R., Mendez, P., Flores, M.P., Rodriguez, R. and Castanon, J.I.R., 2000. Energy and protein content of tedera. CIHEAM Options Mediterraneennes, 45, 219-221.
• Yan, Y., Li, X., Zhang, C., Lv, L., Gao, B. and Li, M., 2021. Research Progress on Antibacterial Activities and Mechanisms of Natural Alkaloids: A Review. Antibiotics, 10(3), 1-30. https://doi.org/10.3390/antibiotics10030318 Yeşilbağ, D., 2007. Fitobiyotikler. Journal of Research in Veterinary Medicine, 26, 33-39.
• Yunusoğlu, O., Berköz, M. and Yardım, Y., 2020. Çoklu antibiyotik direnci gösteren bakterilere karşı geliştirilen yeni antibiyotikler; dalbavansin, telavansin ve oritavansin. Van Sağlık Bilimleri Dergisi, 13(2), 45-54.