Amino-üre Grubu ile Fonksiyonelleştirilmiş Yeni Bir Nanotaşıyıcının Sentezi, Karakterizasyonu ve İlaç Salım Özelliklerinin İncelenmesi

Year 2024, , 1094 - 1101, 01.10.2024

Amelya Gök , Süleyman Akif Demirel , Emine Özkan , Osman Tayyar Arlı

https://doi.org/10.35414/akufemubid.1477648

Abstract

Kurkumin, anti-kanser özelliklerine sahip yaygın bir doğal üründür, ancak düşük çözünürlüğü, vücuttaki etki yerine ulaşma hızının yavaş olması ve kararsızlığı nedeniyle kanser araştırmalarındaki kullanımı sınırlıdır. Kurkuminin kanser tedavisindeki kullanımında karşılaşılan sorunların üstesinden gelmek ve tedavi süresince etkinliğini artırmak amacıyla ilaç taşıyıcı sistemlerin kullanılması çözüm olarak ortaya çıkmıştır. Yapılan bu çalışmada, ilk olarak silika taşıyıcılar arasında en yaygın olan MCM-41 malzemesi hazırlanmıştır. Daha sonra MCM-41 malzemesinin üre-amin grubu ile türevlendirilmesi sonrası MCM-41-AG malzemesi elde edilmiştir. Hazırlanan bu silika taşıyıcı sistemler kullanılarak kurkumin yükleme ve salım çalışmaları gerçekleştirilmiştir. Kurkumin yüklemesi, silika taşıyıcılar ile kurkumin çözeltisinin 24 saat karanlıkta karıştırılması ile gerçekleştirilmiştir. Bu işlem sonrası, kurkumin@MCM-41 ve kurkumin@MCM-41-AG olarak adlandırılan kurkumin yüklü malzemeler elde edilmiştir. Yapılan ölçümlerden kurkumin yükleme yüzdesi, MCM-41 için %14 ve türevlendirilmiş MCM-41-AG için %16,7 olarak hesaplanmıştır. Kurkumin@MCM-41 ve kurkumin@MCM-41-AG malzemelerinden pH 5.5 ve pH 7.4’de kurkumin salımı çalışılmıştır. Yapılan ölçümler sonrası, kurkumin@MCM-41’in her iki pH değerinde de taşıdığı kurkuminin %90’nını ilk 15 dakika içinde bıraktığı belirlenmiştir. Kurkumin@MCM-41-AG taşıdığı kurkuminin pH 5.5’de %31’ni 75 dakika içinde ve pH 7.4’de %39’nu 60 dakika içinde bıraktığı belirlenmiştir. Elde edilen bu sonuçlar, türevlendirilmiş silika taşıyıcı MCM-41-AG’in çıplak MCM-41’e kıyasla daha fazla kurkumin taşıyabildiği ve taşıdığı kurkumini daha az oranda ve daha uzun sürede kontrollü olarak bırakabildiğini göstermektedir.

Keywords

İlaç salım, Kanser, Kurkumin, Nanotaşıyıcı

Ethical Statement

Yazarlar tüm etik standartlara uyduklarını beyan ederler.

Thanks

AG, SAD ve EÖ, bu çalışma sırasında laboratuvar imkanlarını kullanmalarına izin veren Burdur Mehmet Akif Ersoy Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü’ne ve çalışmalar sırasında desteğini esirgemeyen Burdur Ayşe Sak Ortaokulu idarecilerine ve öğretmenlerine teşekkür eder.

Synthesis, Characterization and Investigation of Drug Release Properties of A New Nanocarrier Functionalized with Amino-urea Group

Abstract

Curcumin is a common natural product with anti-cancer properties, but its use in cancer research is limited due to its low solubility, slow rate of action at the site of action in the body, and instability. The use of drug delivery systems has emerged as a solution to overcome the problems encountered in the use of curcumin in cancer treatment and to increase its effectiveness during treatment. In this study, MCM-41 material, which is the most common among silica carriers, was first prepared. Then, MCM-41-AG material was obtained after derivatization of MCM-41 material with urea-amine group. Curcumin loading and release studies were carried out using these prepared silica carrier systems. Curcumin loading was carried out by mixing silica carriers and curcumin solution in the dark for 24 hours. After this process, curcumin-loaded materials called curcumin@MCM-41 and curcumin@MCM-41-AG were obtained. From the measurements, the curcumin loading percentage was calculated as 14% for MCM-41 and 16.7% for derivatized MCM-41-AG. Curcumin release from curcumin@MCM-41 and curcumin@MCM-41-AG materials was studied at pH 5.5 and pH 7.4. After the measurements, it was determined that 90% of the curcumin carried by curcumin@MCM-41 at both pH values was released within the first 15 minutes. It was determined that the curcumin carried by curcumin@MCM-41-AG released 31% within 75 minutes at pH 5.5 and 39% within 60 minutes at pH 7.4. These results show that the derivatized silica carrier MCM-41-AG can carry more curcumin compared to bare MCM-41 and release the curcumin it carries in a controlled manner at a lower rate and for a longer period of time.

Keywords

Cancer, Curcumin, Drug release, Nanocarrier

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