Rumex Crispus L.’dan Sekonder Metabolitlerin Kromatografik Yöntemlerle İzolasyonu ve Tayini

Year 2024, , 1305 - 1312, 02.12.2024

Elif Günay , Dilek Mutlu , Deniz Küpçük , Gizem Yıldırım Baştemur , Sabriye Perçin Özkorucuklu

https://doi.org/10.35414/akufemubid.1483597

Abstract

İnsanlar binlerce yıldır bitkilerin sağlık açısından faydalı özelliklerinden yararlanmaktadır. Günümüzde, tıp biliminin yanı sıra başta kozmetik endüstrisinde olmak üzere birçok sektörde bitkiler kullanılmaktadır ve bitkilerin fitokimyasal içeriklerinin belirlenmesine ilişkin çalışmalar da hızla artmaktadır. Halk arasında çeşitli amaçlarla kullanılan Rumex crispus L. bitkisi özellikle fenolik bileşikler ve antrakinonlar olmak üzere zengin sekonder metabolit içeriğinden dolayı dikkat çekmektedir. Bu çalışmada Türkiye’de yetişen Rumex crispus L. bitkisinden katma değere sahip fenolik bileşik ve antrakinonların izolasyonu pratik ve ekonomik bir metot olan gravity kolon kromatografisi ile gerçekleştirilmiştir. Ekstrakt apolar çözücü sistemden polar çözücü sistemine doğru fraksiyonlara ayrılmış ve bu fraksiyonlardaki fenolik bileşik ve antrakinon miktarları ters faz sıvı kromatografi (RPLC) yöntemi ile belirlenmiştir. Protokateşik asit, gentisik asit, kafeik asit, p-kumarik asit, polydatin, kumarin, resveratrol ve ellajik asit izolasyonu için etil asetat-metanol (6:4) çözücü sisteminin uygun olduğuna karar verilmiştir. Sonuç olarak, bitkilerdeki sekonder metabolitlerin araştırılmasında maliyeti düşük olan gravity kolon kromatografisi kullanılarak fenolik bileşiklerin ve antrakinonların saflaştırılabileceği ortaya konulmuştur.

Keywords

Rumex crispus L., Gravity kolon kromatografi, RPLC, Fenolik bileşik, Antrakinon.

Ethical Statement

Yazarlar tüm etik standartlara uyduklarını beyan ederler.

Supporting Institution

İstanbul Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

FLO-2023-40354

Thanks

Bu çalışma İstanbul Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir (Proje numarası: FLO-2023-40354). İstanbul Üniversitesi Fen Fakültesi Botanik Anabilim Dalından Doç. Dr. İbrahim Sırrı Yüzbaşıoğlu’na bitki tür tayinindeki katkılarından dolayı teşekkür ederiz.

Isolation and Determination of Secondary Metabolites from Rumex Crispus L. by Chromatographic Methods

Abstract

People have been benefiting from the health benefits of plants for thousands of years. Nowadays, plants are used in many sectors, especially in the cosmetic industry, as well as medical science, and studies on the determination of the phytochemical contents of plants are rapidly increasing. Rumex crispus L., a plant commonly used for various purposes by the public, attracts attention due to its rich secondary metabolite content, especially phenolic compounds and anthraquinones. In this study, the isolation of valuable phenolic compounds and anthraquinones from the Rumex crispus L. plant grown in Turkey was carried out by gravity column chromatography, which is a practical and economical method. The extract was divided into fractions from the nonpolar solvent system to the polar solvent system, and the amounts of phenolic compounds and anthraquinones in these fractions were determined by the reverse phase liquid chromatography (RPLC) method. It was decided that the ethyl acetate-methanol (6:4) solvent system was suitable for the isolation of protocatechuic acid, gentisic acid, caffeic acid, p-coumaric acid, polydatin, coumarin, resveratrol and ellagic acid. Consequently, it has been revealed that phenolic compounds and anthraquinones can be purified by using low-cost gravity column chromatography in the investigation of secondary metabolites in plants.

Keywords

Rumex crispus L., Gravity column chromatography, RPLC, Phenolic compound, Anthraquinone.

Project Number

FLO-2023-40354

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