Potential Mutagenicity of Udimo 75 WG Herbicide in Salmonella typhimurium with Ames Test

Year 2021, , 1016 - 1021, 31.10.2021

Dilek Akyıl

https://doi.org/10.35414/akufemubid.891586

Abstract

In addition to the benefits of pesticides frequently used in the agricultural sector, there are also many negative effects on the environment and living organisms. We aimed this study was to investigate mutagenic activity of Udimo 75 WG herbicide that commonly used in the agricultural sector by Ames/Salmonella microsome short-time test system. Experiments were applied in the absence (-S9) and presence (+S9) of enzymes in the metabolic activation by using TA98 and TA100 strains of Salmonella typhimurium. At first, the cytotoxic doses of the test substances were detected and then, 5 non-cytotoxic dose for each test substances were selected. For the mutagenicity of Udimo 75 WG 500, 250, 125, 62.5 and 31.25 μg/plate doses were studied. The obtained data were statistically analysed by Dunnett's t-test and the results were evaluated by compare with spontaneous control plates. According to the results of this study, Udimo 75 WG herbicide showed mutagenic activity both in the absence and presence of S9 fraction at the dose of 500 μg/plate concentration in both strains. Therefore, this herbicide may be in the genotoxic risk group for humans and other living things.

Keywords

Ames test, Mutagenicity, Sulfonylurea herbicides, Udimo 75 WG.

Udimo 75 WG Herbisitinin Ames Testi ile Salmonella typhimurium'da Potansiyel Mutajenitesi

Abstract

Tarım sektöründe sıklıkla kullanılan pestisitlerin faydalarının yanı sıra çevreye ve yaşayan canlılara olumsuz birçok etkisi de vardır. Bu çalışmada, tarım sektöründe yaygın olarak kullanılan bir herbisit olan Udimo 75 WG’nin Ames/Salmonella mikrozom kısa süreli test sistemi ile mutajenik aktivitesinin araştırılması amaçlanmıştır. Deneyler Salmonella typhimurium TA98 ve TA100 suşları kullanılarak metabolik aktivasyon yokluğunda (-S9) ve varlığında (+S9) gerçekleştirilmiştir. İlk olarak test maddelerinin sitotoksik dozları tespit edilmiş ve ardından her test maddesi için 5 sitotoksik olmayan doz seçilmiştir. Çalışmada Udimo 75 WG 500, 250, 125, 62.5 ve 31.25 μg/plak dozları çalışılmıştır. Elde edilen veriler, Dunnett t-testi ile istatistiksel olarak analiz edilmiş ve sonuçlar, spontan kontrol plakları ile karşılaştırılarak değerlendirilmiştir. Bu çalışmanın sonuçlarına göre, Udimo 75 WG herbisiti, her iki suşta da 500 μg/plak konsantrasyonunda S9 fraksiyonunun hem yokluğunda hem de varlığında mutajenik aktivite göstermiştir. Dolayısıyla bu herbisit, insanlar için genotoksik bir risk oluşturabilir.

Keywords

Ames testi, Mutajenite, Sülfonilüre herbisitleri, Udimo 75 WG

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