Optimization of Bioprotein Production by Pleurotus ostreatus in Submerged Culture Conditions

Year 2013, Volume: 13 Issue: 1, 35 - 42, 01.04.2013

Cem Özkan Mustafa Yamaç Zeki Yıldız

https://doi.org/10.5578/fmbd.5905

Cited By: 1

Abstract

In this study, the effects of environmental factors on mycelium development of Pleurotus ostreatus was investigated. Besides, optimum nutritional factors for bioprotein production by the isolate were selected by Plackett-Burman design. Temperature of 30 °C, pH 8 and 4% inoculant size were selected among 7 incubation temperature (15-45 °C), 9 pH values (4.0-8.0), and 9 inoculant size (1-16%) for maximum mycelial growth of the isolate. In the case of Pleurotus ostreatus, dog food, lactose, corn steep liquor, gibberellic acid, CaCl2 and KH2POsignificantly influenced the bioprotein production. © Afyon Kocatepe Üniversitesi

Keywords

-

Pleurotus ostreatus Makrofungusu ile Derin Kültür Koşullarında Biyoprotein Üretiminin Optimizasyonu

Abstract

Bu çalışmada, Pleurotus ostreatus makrofungus izolatının misel gelişimi üzerine etki eden çevresel faktörler belirlenmiştir. Ayrıca izolatın biyoprotein üretimi için en uygun besinsel bileşenler PlackettBurman yöntemi ile seçilmiştir. Araştırma sonucunda 7 farklı inkübasyon sıcaklığı (15–45 °C), 9 farklı pH derecesi (4.0-8.0) ve 9 farklı inokülant miktarı (1-16 %) arasından en iyi misel gelişimi gözlenen sıcaklık 30 °C, pH 8 ve inokülant miktarı % 4 olarak belirlenmiştir Köpek yemi, laktoz, corn steep liquor, giberellik asit, CaCl2 ve KH2PO4 içerikli besiyerinin Pleurotus ostreatus izolatının biyoprotein üretimini önemli oranda arttırdığı belirlenmiştir

Keywords

Biyoprotein, Derin kültür, Pleurotus ostreatus, PlackettBurman

References

• Adebayo-Tayo B.C., Jonathan S.G., Popoola O.O. and Egbomuche R.C. 2011. Optimization of growth conditions for mycelial yield and exopolysaccharride production by Pleurotus ostreatus cultivated in Nigeria. African Journal of Microbiology Research. 5, 2130-2138.
• Anonymous, 2011. World population to 2300, United Nations, Department of Economic and Social Affairs, Population Division Reports, 240 pp.
• Breitenbach, J. and Kranzlin, F., 1986. Fungi of Switzerland, Vol. II. Non Gilled Fungi, Verlag Mykologia, 412 pp.
• Fasiola T.R., Gbolagade J.S. and Fasidi I.O., 2007. Nutritional requirements of Volvariella speciosa (Fr. Ex. Fr.) Singer, a Nigerian edible mushroom. Food Chemistry, 100, 904–908.
• Gern, R.M.M., Wisbeck, E., Rampinelli, J.R., Ninow, J.L. and Furlan, S.A., 2008. Alternative medium for production of Pleurotus ostreatus biomass and potential antitumor polysaccharides. Bioresource Technology, 99, 76–82.
• Gbolagade J.S., Fasidi I.O., Ajayi E.J. and Sobowale A.A., 2006a. Effect of physico-chemical factors and semi- synthetic media on vegetative growth of Lentinus subnudus (Berk.), an edible mushroom from Nigeria. Food Chemistry, 99, 742–747.
• Gbolagade, J., Sobowal, A. and Adejoy, D., 2006b. Optimization of submerged culture conditions for biomass production in Pleurotus florida (Mont.) Singer, a Nigerian edible fungus. African Journal of Biotechnology, 5, 1464-1469.
• Gregori, A., Svagelj, M., and Pohleven, J., 2007. Cultivation Techniques and Medicinal Properties of Pleurotus spp. Food Technology and Biotechnology, 45, 238–249.
• Hanai H., Ishida S., Saito C., Maita T., Kusano M., Tamogami S. and Noma M., 2005. Stimulation of mycelia growth in several mushroom species by rice husks. Bioscience Biotechnology and Biochemistry, 69, 123-127.
• Jonathan S.G. and Fasidi I.O., 2001. Studies on phytohormones, vitamins and mineral element requirements of Lentinus subnudus (Berk) and Schizophyllum commune (Fr. Ex. Fr) from Nigeria. Food Chemistry, 75, 303-307.
• Jonathan S.G. and Fasidi I.O., 2003. Studies on Psathyerella atroumbonata (Pegler), a Nigerian edible fungus. Food Chemistry, 81, 481-484.
• Jonathan S.G. Fasidi I.O. and Ajayi E.J., 2004. Physico- Chemical studies on Volvariella esculenta (Mass) Singer, a Nigerian edible fungus. Food Chemistry, 85, 339-342.
• Jonathan S.G., Bawo D.D.S., Adejoye D.O. and Briyai O.F., 2009. Studies on Biomass Production in Auricularia polytricha Collected from Wilberforce Island, Bayelsa State, Nigeria. American Journal of Applied Sciences, 6, 182-186.
• Kawagishi H., Hamajima K., Takanami R., Nakamura T., Sato Y., Akiyama Y., Sano M. and Tanaka O., 2004. Growth promotion of mycelia of the Matsutake mushroom Tricholoma matsutake by D-isoleucine, Bioscience Biotechnology and Biochemistry, 68, 2405-2407.
• Kurbanoglu E.B. Algur O.F. and Zulkadir A., 2004. Submerged production of edible mushroom Agaricus bisporus mycelium in ram horn hydrolysate, Industrial Crops and Products, 19, 225-230.
• Le J., Hu S. and Xu M. 2007. Optimization of submerged culture conditions fort he production of mycelial biomass and exopolysaccharide by Pleurotus nebrodensis. Annals of Microbiology. 57, 389-393.
• Lee B.C., Bae J.T., Pyo H.B., Choe T.B., Kim S.W., Hwang H.J. and Yun J.W. 2004. Submerged culture conditions for the production of mycelial biomass and exopolysaccharides by the edible Basidiomycete Grifola frondosa. Enzyme and Microbial Technology, 35, 369-376.
• Moser, M., 1978. Keys to Agarics and Boleti, Roger Philipps, Stuttgard, 535 pp.
• Mukhopadhyay R., Chatterjee S., Chatterjee B.P. and Guha A.K., 2005. Enhancement of biomass production of edible mushroom Pleurotus sajor-caju grown in whey by plant growth hormones. Process Biochemistry, 40, 1241–1244.
• Örücü E. ve Kanıbir H., 2005. Yumurta Pazarındaki Sorunlara Pazarlama Anlayışı Perspektifinden Bir Bakış ve Çözüm Önerisi, Balıkesir Üniversitesi SBE Dergisi, 8, 145-164.
• Özkan, C., Bahadır, A., Karaduman, A.B., Özbulut, N., Yamaç, M., 2011. Polyporus squamosus (Huds.) Fr. OBCC 5003 suşu ile biyomas üretimi üzerine çalışmalar, Journal of Fungus (Mantar Dergisi), 2(1- 2), 37-47.
• Özkan, C. ve Yamaç, M., 2012. Bazı Yenebilir Makrofungus İzolatlarının Biyoprotein Üretimi Üzerine Sıcaklığın Etkisi, IX. Türkiye Yemeklik Mantar Kongresi, 18-20 Ekim 2012, Denizli.
• Papaspyridi L.M., Katapodis P., Gonou-Zagou Z., Kapsanaki-Gotsi E. and Christakopoulos P. 2010. Optimization of biomass production with enhanced glucan and dietary fibres content by Pleurotus ostreatus ATHUM 4438 under submerged culture. Biochemical Engineering Journal, 50, 131-138.
• Plackett, R.L. and Burman, J.P. 1946. The design of optimum multifactorial experiments. Biometrika, 33, 305-325.
• Postemsky P., Gonzalez Matute R., Figlas D., and Curvetto N., 2006. Optimizing Grifola sordulenta and Grifola gargal growth in agar and liquid nutrient media. Mycologica Aplicata Internazionale, 18, 7-12.
• Wu J.Z., Cheung P.C.K., Wong K.H. and Huang N.L., 2003. Studies on submerged fermentation of Pleurotus tuber-regium (Fr.) Singer—Part 1: physical and chemical factors affecting the rate ofmycelial growth and bioconversion efficiency. Food Chemistry, 81, 389-393.