Comparison of 16S-ITS rDNA RFLP Profiles of Bacillus sp. Isolated from Milk and Different Water Sources (021004) (29-38)

Year 2014, Volume: 14 Issue: 2, 29 - 38, 01.08.2014

Şakire Hanoğlu S. Elif Korcan S. Feyza Erdoğmuş Muhsin Konuk

Abstract

Bu çalışmanın amacı farklı kaynaklardan izole edilen termofilik Bacillus sp.'ları 16S-ITS rDNA RFLP ile gruplandırmaktır. Çeşitli çevresel örneklerden yetmiş dört suş izole edilmiştir. Gram (+), endospor oluşturan, basil şeklindeki izolatlar Bacillus olarak identifiye edilmiştir Bu suşlar; lipaz, amilaz, proteaz, ksilinaz ve selulazdan oluşan ekstraselülar beş enzim aktivitesi açısından incelenmiştir. 16S-ITS rDNA RFLP profilleri TaqI ve HaeIII olmak üzere iki farklı restriksiyon enzimi kullanılarak çıkarılmıştır. İzolatlar TaqI ile sekiz, HaeIII ile yedi farklı restriksiyon grubu oluşturmuştur

Keywords

Bacillus sp.; Ekstraselüler enzim; 16S-ITS rDNA; RFLP

Farklı Su ve Süt Kaynaklarından İzole Edilen Bacillus sp.'ların 16S-ITS rDNA RFLP Profillerinin Karşılaştırılması

Abstract

The aim of this research was the isolation of thermophilic Bacillus sp. from different sources and grouping by 16S-ITS rDNA RFLP. Seventy-four thermophilic strains were isolated from various environmental samples. Since fifteen isolated strains were Gram (+), endospore forming rods, they were identified as Bacillus sp. These strains were screened for the existence of five extracellular enzyme activities. These were, lipases, amylases, proteases, xylanases, cellulases. 16S-ITS rDNA RFLP profiles were produced by using two restriction endonucleases Taq I and Hae III. The isolated strains were clustered into eigth groups by Taq I restriction profiles of 16S-ITS rDNA while seven groups were obtained by Hae III digestion profiles

Keywords

Bacillus sp.; Extracellular enzyme; 16S-ITS rDNA; RFLP

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