UV-C Stresinin 6-Benzilaminopürin Varlığında Çoğaltılmış Amsonia orientalis’in In vitro Sürgün Gelişimi ve Antioksidan Enzimleri Üzerine Etkileri

Year 2018, Volume: 18 Issue: 1, 44 - 52, 30.04.2018

Yonca Yüzügüllü Karakuş , Halil İ. Toygar Arda Acemi Yonca Avcı Duman Fazıl Özen

Abstract

Bu çalışmada abiyotik faktörlerden biri olan UV-C stresinin 6-benzilaminopürin varlığında çoğaltılmış Amsonia orientalis’in in vitro sürgünleri üzerindeki etkileri, sürgünlerin morfometrik özellikleri, H2O2 (hidrojen peroksit) ve MDA (malondialdehit) seviyeleri ile antioksidan enzimleri analiz edilerek irdelenmiştir. Test edilen 3,47 (15 dk), 6,94 (30 dk) ve 13,87 (60 dk) kJ m-2’lik UV-C radyasyon dozlarının hepsi sürgün uzunluğu ile sürgün sayısında kontrole (UV-C uygulanmamış bitki) oranla düşüşe sebep olmuştur. Radyasyon kaynaklı oluşan bu stres 6-benzilaminopürin varlığında sürgün gelişimini yavaşlatsa da tamamen durdurmamıştır. UV-C radyasyon uygulamaları antioksidan enzimler üzerine farklı etkiler göstermiştir. SOD (süperoksit dismutaz) aktivitesi artan radyasyon dozuna bağlı olarak kontrole göre önemli ölçüde azalırken, POD (peroksidaz) aktivitesinde artış gözlenmiştir. CAT (katalaz) aktivitesinin ise radyasyondan önemli ölçüde etkilenmediği görülmüştür. Artan UV-C dozlarında H2O2 ve MDA seviyelerinin benzer olarak arttığı bulunmuştur. Elektroforetik analizler sonucunda SOD enzimi için üç farklı izozim (Cu/Zn-SOD, Fe-SOD ve Mn-SOD) tespit edilmiş olup, artan UV-C stresinin bitkinin Cu/Zn-SOD aktivitesini tamamen inhibe ettiği gözlenmiştir. Sonuçlar bitkinin UV stresine karşı gösterdiği savunma mekanizmasında peroksidazların incelenen diğer antioksidan enzimlere göre öncelikli rol aldığına işaret etmektedir.

Keywords

Amsonia orientalis, katalaz, peroksidaz, süperoksit dismutaz, UV stresi

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