Farklı Ortam Koşullarının Micrococcus sp. Ekzopolisakkarit Üretimine Etkisi

Year 2019, Volume: 19 Issue: 1, 40 - 46, 28.05.2019

NUR Koçberber Kılıç , Gönül Dönmez

https://doi.org/10.35414/akufemubid.387249

Cited By: 1

Abstract

Endüstriyel atıksulardan izole edilen Micrococcus
sp. ile yapılan çalışmada, farklı pH değerlerinin, Remazol Blue
konsantrasyonlarının, sıcaklık derecelerinin ve inkübasyon sürelerinin
bakterinin ürettiği ekzopolisakkarit (EPS) miktarına etkisi araştırılmıştır.
Remazol Blue içeren ortamlarda pH 6-9 aralığında 30 °C de yapılan denemelerde, pH 6’da yüksek miktarda EPS
üretildiği belirlenmiştir. Bakteri 100-400 mg/l arasındaki başlangıç Remazol
Blue konsantrasyonlarında (pH 6, 30 °C) en iyi EPS üretimini, 200 mg/l Remazol Blue konsantrasyonunda 234.8
mg/l olarak yapmıştır. Artan sıcaklık derecelerinin Micrococcus sp.
tarafından üretilen EPS miktarlarına etkisinin araştırıldığı denemelerde ise en
yüksek EPS üretimine 40 °C’de
ulaşıldığı görülmüştür. İnkübasyon süresinin üretilen EPS miktarına etkisi
araştırıldığında, artan inkübasyon süresinin EPS üretimini azalttığı, en yüksek
EPS üretimine biyokütle üretiminin yeni başladığı 48 saatlik inkübasyon süresi
sonunda ulaşıldığı belirlenmiştir. Denemeler sonunda en yüksek EPS üretimi, pH
6’ da, 100 mg/l Remazol Blue içeren besiyerinde, 40 °C’de ve 48 saat inkübasyon süresi sonrasında 257.2 mg/l
olarak elde edilmiştir. 

Keywords

Micrococcus sp., Ekzopolisakkarit (EPS), Remazol Blue

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