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Kükürt İçeren Ligand ve Bakır Kompleksine Ait Antioksidan ve Topoizomeraz I İnhibitör Aktivitelerin Karşılaştırılması

Year 2020, Volume: 20 Issue: 1, 30 - 34, 17.03.2020
https://doi.org/10.35414/akufemubid.629432

Abstract

Bu çalışmada antikanser
ve antioksidan ajan olarak kullanılabilecek dtb ligandı ve bu ligantın
[Cu(dtb)(phen)]2+ kompleksi ile çalışılmıştır. Antioksidan
aktivitelerinin belirlenmesi için DPPH radikali süpürme yöntemi kullanılmıştır.
Dtb ligandı düşük antioksidan aktivite gösterirken bu ligantın Cu(II)
kompleksinin iyi bilinen bir antioksidan olan C vitamini kadar yüksek
antioksidan aktivite gösterdiği görülmüştür. Topoizomeraz-I inhibisyonu
aktivitesi çalışmasında pBR322 plazmid DNA’sının relaksasyonu agaroz jel
elektroforez yöntemiyle takip edilmiştir. Antioksidan aktivitede olduğu gibi
topoizomeraz-I inhibisyonu aktivitesinde de dtb ligandı düşük etki gösterirken
bakır kompleksi µM gibi düşük derişimde bile inhibisyon gerçekleştirebilmiştir.
[Cu(dtb)(phen)]2+ kompleksinin antikanser ve antioksidan ajan olarak
kullanılabilme potansiyelinin yüksek olduğu görülmüştür. 

Supporting Institution

ZONGULDAK BÜLENT ECEVİT ÜNİVERSİTESİ

References

  • Bhaskara Reddy, M. V., Srinivasulu, D., Peddanna, K., Apparao, C. and Ramesh, P., 2015. Synthesis and Antioxidant Activity of New Thiazole Analogues Possessing Urea, Thiourea, and Selenourea Functionality. Synthetic Communications, 45, 2592-2600.
  • Blois, M. S., 1958. Antioxidant Determinations by the Use of a Stable Free Radical. Nature, 181, 1199-1200.
  • Chen, W., Sun, S., cao, W., Liang, Y. and Song, J., 2009. Antioxidant property of quercetin–Cr(III) complex: The role of Cr(III) ion. Journal of Molecular Structure, 918, 194-197.
  • Cornelli, U., 2009. Antioxidant use in nutraceuticals. Clinics in Dermatology, 27, 175-194.
  • Cuya, S. M., Bjornsti, M.-A. and van Waardenburg, R. C. A. M., 2017. DNA topoisomerase-targeting chemotherapeutics: what’s new? Cancer Chemotherapy and Pharmacology, 80, 1-14.
  • Fortune, J. M., Velea, L., Graves, D. E., Utsugi, T., Yamada, Y. and Osheroff, N., 1999. DNA Topoisomerases as Targets for the Anticancer Drug TAS-103:  DNA Interactions and Topoisomerase Catalytic Inhibition. Biochemistry, 38, 15580-15586.
  • Joseph, J. and Janaki, G. B., 2016. Copper complexes bearing 2-aminobenzothiazole derivatives as potential antioxidant: Synthesis, characterization. Journal of Photochemistry and Photobiology B: Biology, 162, 86-92.
  • Katekhaye, S. D. and Kale, M. S., 2012. Antioxidant and free radical scavenging activity of Pithecellobium dulce (Roxb.) Benth wood bark and leaves. Free Radicals and Antioxidants, 2, 47-57.
  • Martínez Medina, J. J., Naso, L. G., Pérez, A. L., Rizzi, A., Okulik, N. B., Valcarcel, M., Salado, C., Ferrer, E. G. and Williams, P. A. M., 2019. Synthesis, characterization, theoretical studies and biological (antioxidant, anticancer, toxicity and neuroprotective) determinations of a copper(II) complex with 5-hydroxytryptophan. Biomedicine & Pharmacotherapy, 111, 414-426.
  • Montaner, B., Castillo-Ávila, W., Martinell, M., Öllinger, R., Aymami, J., Giralt, E. and Pérez-Tomás, R., 2005. DNA Interaction and Dual Topoisomerase I and II Inhibition Properties of the Anti-Tumor Drug Prodigiosin. Toxicological Sciences, 85, 870-879.
  • Nami, S. A. A., Ullah, I., Alam, M., Lee, D.-U. and Sarikavakli, N., 2016. Synthesis, characterization, molecular docking and biological studies of self assembled transition metal dithiocarbamates of substituted pyrrole-2-carboxaldehyde. Journal of Photochemistry and Photobiology B: Biology, 160, 392-399.Sas, K., Robotka, H., Toldi, J. and Vécsei, L., 2007. Mitochondria, metabolic disturbances, oxidative stress and the kynurenine system, with focus on neurodegenerative disorders. Journal of the Neurological Sciences, 257, 221-239.
  • Soare, J. R., Dinis, T. C. P., Cunha, A. P. and Almeida, L., 1997. Antioxidant Activities of Some Extracts of Thymus zygis. Free Radical Research, 26, 469-478.
  • Srivastava, S., Blower, P. J., Aubdool, A. A., Hider, R. C., Mann, G. E. and Siow, R. C., 2016. Cardioprotective effects of Cu(II)ATSM in human vascular smooth muscle cells and cardiomyocytes mediated by Nrf2 and DJ-1. Scientific Reports, 6, 7.
  • Suzuki, K. and Uyeda, M., 2002. Inhibitory Properties of Antitumor Prostaglandins against Topoisomerases. Bioscience, Biotechnology, and Biochemistry, 66, 1706-1712.
  • Yildiz, U. and Coban, B., 2019. Chemical and photo-induced nuclease activity of a novel minor groove DNA binder Cu(II) complex. 2019, 84, 12.

Comparison of Antioxidant and Topoisomerase I Inhibitor Activities of Sulfur Containing Ligand and Copper Complex

Year 2020, Volume: 20 Issue: 1, 30 - 34, 17.03.2020
https://doi.org/10.35414/akufemubid.629432

Abstract

In this study, dtb ligand and its [Cu (dtb) (phen)]2+ complex which can be used as anticancer and antioxidant agent were studied. DPPH radical scavenging method was used to determine the antioxidant activities. The dtb ligand had low antioxidant activity, while the Cu (II) complex of this ligand had as high antioxidant activity as vitamin C, a well-known antioxidant. In the topoisomerase-I inhibition activity study, relaxation of pBR322 plasmid DNA was followed by agarose gel electrophoresis method. In addition to antioxidant activity, dtb ligand had a low effect on topoisomerase-I inhibition activity, while the copper complex was able to inhibit even at low concentrations such as 10 µM. The obtained results show [Cu (dtb) (phen)]2+ complex has a high potential to be used as an anticancer and antioxidant agent.

References

  • Bhaskara Reddy, M. V., Srinivasulu, D., Peddanna, K., Apparao, C. and Ramesh, P., 2015. Synthesis and Antioxidant Activity of New Thiazole Analogues Possessing Urea, Thiourea, and Selenourea Functionality. Synthetic Communications, 45, 2592-2600.
  • Blois, M. S., 1958. Antioxidant Determinations by the Use of a Stable Free Radical. Nature, 181, 1199-1200.
  • Chen, W., Sun, S., cao, W., Liang, Y. and Song, J., 2009. Antioxidant property of quercetin–Cr(III) complex: The role of Cr(III) ion. Journal of Molecular Structure, 918, 194-197.
  • Cornelli, U., 2009. Antioxidant use in nutraceuticals. Clinics in Dermatology, 27, 175-194.
  • Cuya, S. M., Bjornsti, M.-A. and van Waardenburg, R. C. A. M., 2017. DNA topoisomerase-targeting chemotherapeutics: what’s new? Cancer Chemotherapy and Pharmacology, 80, 1-14.
  • Fortune, J. M., Velea, L., Graves, D. E., Utsugi, T., Yamada, Y. and Osheroff, N., 1999. DNA Topoisomerases as Targets for the Anticancer Drug TAS-103:  DNA Interactions and Topoisomerase Catalytic Inhibition. Biochemistry, 38, 15580-15586.
  • Joseph, J. and Janaki, G. B., 2016. Copper complexes bearing 2-aminobenzothiazole derivatives as potential antioxidant: Synthesis, characterization. Journal of Photochemistry and Photobiology B: Biology, 162, 86-92.
  • Katekhaye, S. D. and Kale, M. S., 2012. Antioxidant and free radical scavenging activity of Pithecellobium dulce (Roxb.) Benth wood bark and leaves. Free Radicals and Antioxidants, 2, 47-57.
  • Martínez Medina, J. J., Naso, L. G., Pérez, A. L., Rizzi, A., Okulik, N. B., Valcarcel, M., Salado, C., Ferrer, E. G. and Williams, P. A. M., 2019. Synthesis, characterization, theoretical studies and biological (antioxidant, anticancer, toxicity and neuroprotective) determinations of a copper(II) complex with 5-hydroxytryptophan. Biomedicine & Pharmacotherapy, 111, 414-426.
  • Montaner, B., Castillo-Ávila, W., Martinell, M., Öllinger, R., Aymami, J., Giralt, E. and Pérez-Tomás, R., 2005. DNA Interaction and Dual Topoisomerase I and II Inhibition Properties of the Anti-Tumor Drug Prodigiosin. Toxicological Sciences, 85, 870-879.
  • Nami, S. A. A., Ullah, I., Alam, M., Lee, D.-U. and Sarikavakli, N., 2016. Synthesis, characterization, molecular docking and biological studies of self assembled transition metal dithiocarbamates of substituted pyrrole-2-carboxaldehyde. Journal of Photochemistry and Photobiology B: Biology, 160, 392-399.Sas, K., Robotka, H., Toldi, J. and Vécsei, L., 2007. Mitochondria, metabolic disturbances, oxidative stress and the kynurenine system, with focus on neurodegenerative disorders. Journal of the Neurological Sciences, 257, 221-239.
  • Soare, J. R., Dinis, T. C. P., Cunha, A. P. and Almeida, L., 1997. Antioxidant Activities of Some Extracts of Thymus zygis. Free Radical Research, 26, 469-478.
  • Srivastava, S., Blower, P. J., Aubdool, A. A., Hider, R. C., Mann, G. E. and Siow, R. C., 2016. Cardioprotective effects of Cu(II)ATSM in human vascular smooth muscle cells and cardiomyocytes mediated by Nrf2 and DJ-1. Scientific Reports, 6, 7.
  • Suzuki, K. and Uyeda, M., 2002. Inhibitory Properties of Antitumor Prostaglandins against Topoisomerases. Bioscience, Biotechnology, and Biochemistry, 66, 1706-1712.
  • Yildiz, U. and Coban, B., 2019. Chemical and photo-induced nuclease activity of a novel minor groove DNA binder Cu(II) complex. 2019, 84, 12.
There are 15 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Articles
Authors

Ufuk Yıldız 0000-0002-0419-0011

Publication Date March 17, 2020
Submission Date October 4, 2019
Published in Issue Year 2020 Volume: 20 Issue: 1

Cite

APA Yıldız, U. (2020). Kükürt İçeren Ligand ve Bakır Kompleksine Ait Antioksidan ve Topoizomeraz I İnhibitör Aktivitelerin Karşılaştırılması. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi, 20(1), 30-34. https://doi.org/10.35414/akufemubid.629432
AMA Yıldız U. Kükürt İçeren Ligand ve Bakır Kompleksine Ait Antioksidan ve Topoizomeraz I İnhibitör Aktivitelerin Karşılaştırılması. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi. March 2020;20(1):30-34. doi:10.35414/akufemubid.629432
Chicago Yıldız, Ufuk. “Kükürt İçeren Ligand Ve Bakır Kompleksine Ait Antioksidan Ve Topoizomeraz I İnhibitör Aktivitelerin Karşılaştırılması”. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi 20, no. 1 (March 2020): 30-34. https://doi.org/10.35414/akufemubid.629432.
EndNote Yıldız U (March 1, 2020) Kükürt İçeren Ligand ve Bakır Kompleksine Ait Antioksidan ve Topoizomeraz I İnhibitör Aktivitelerin Karşılaştırılması. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi 20 1 30–34.
IEEE U. Yıldız, “Kükürt İçeren Ligand ve Bakır Kompleksine Ait Antioksidan ve Topoizomeraz I İnhibitör Aktivitelerin Karşılaştırılması”, Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi, vol. 20, no. 1, pp. 30–34, 2020, doi: 10.35414/akufemubid.629432.
ISNAD Yıldız, Ufuk. “Kükürt İçeren Ligand Ve Bakır Kompleksine Ait Antioksidan Ve Topoizomeraz I İnhibitör Aktivitelerin Karşılaştırılması”. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi 20/1 (March 2020), 30-34. https://doi.org/10.35414/akufemubid.629432.
JAMA Yıldız U. Kükürt İçeren Ligand ve Bakır Kompleksine Ait Antioksidan ve Topoizomeraz I İnhibitör Aktivitelerin Karşılaştırılması. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi. 2020;20:30–34.
MLA Yıldız, Ufuk. “Kükürt İçeren Ligand Ve Bakır Kompleksine Ait Antioksidan Ve Topoizomeraz I İnhibitör Aktivitelerin Karşılaştırılması”. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi, vol. 20, no. 1, 2020, pp. 30-34, doi:10.35414/akufemubid.629432.
Vancouver Yıldız U. Kükürt İçeren Ligand ve Bakır Kompleksine Ait Antioksidan ve Topoizomeraz I İnhibitör Aktivitelerin Karşılaştırılması. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi. 2020;20(1):30-4.