Eisenia hortensis Sölomositlerinde Bentazone ve Chloridazon Herbisitlerinin Genotoksisitesinin Değerlendirilmesi

Year 2020, Volume: 20 Issue: 6, 963 - 967, 31.12.2020

Sevgi Ulukütük İbrahim Hakkı Ciğerci

https://doi.org/10.35414/akufemubid.827228

Cited By: 1

Abstract

Bu çalışmada, Bentazone ve Chloridazon herbisitlerinin Eisenia hortensis türleri üzerindeki genotoksik etkileri araştırılmıştır. E. hortensis türleri Afyonkarahisar'daki doğal yaşam alanlarından toplanmıştır. Solucan sölomositlerinde genotoksisiteyi değerlendirmek için Comet ve Mikronükleus testi yapıldı. Bentazone herbisitlerin LD50 değeri Chloridazon için sırasıyla 236 ppm ve 76.6 ppm olarak kaydedildi. Daha sonra LD50/2, LD50 and 2XLD50 konsantrasyonlarında Bentazon ve Chloridazon herbisitleri E. hortensis'e 48 saat süreyle uygulandı. Her iki herbisitte de DNA'da konsantrasyona bağlı artış ve kromozomal hasar gözlendi (P <0.05). En yüksek DNA hasarı ve kromozomal sapmaları, diğer konsantrasyonlara ve kontrol grubuna kıyasla en yüksek dozlarda tespit edildi. Bentazone ve Chloridazon'un E. hortensis’ te DNA hasarı ve kromozomal sapmalara neden olduğu sonucuna varılmıştır.

Keywords

Bentazon, Chloridazon, Eisenia hortensis, Genotoksisite, Comet yöntemi, Mikronukleus

Supporting Institution

Afyon Kocatepe Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

15.FEN.BİL.44

Genotoxic Evaluation of Bentazone and Chloridazon Herbicides in Eisenia hortensis Coelomocytes

Abstract

In current study, genotoxic effects of Bentazone and Chloridazon herbicides on Eisenia hortensis species were investigated. The species of E. hortensis were collected from the natural habitats in Afyonkarahisar. Comet assay and Micronucleus test was conducted to evaluate the genotoxicity in earthworm coelomocytes. The LD50 value of Bentazone herbicides was noted as 236 ppm and 76.6 ppm for Chloridazon, respectively. Then LD50/2, LD50 and 2XLD50 concentrations of Bentazone and Chloridazon herbicides were applied to E. hortensis for 48 h. Concentration dependent increase in DNA and chromosomal damage was observed (P < 0.05) by both herbicides. Highest DNA damage and micronucleus formation were noticed at highest doses compared to other concentrations and control group. It was concluded that Bentazone and Chloridazon induced DNA damage and chromosomal aberrations failure in E. hortensis earthworms.

Keywords

Bentazone, Chloridazon, Chloridazon, Eisenia hortensis, Eisenia hortensis, Genotoxicity, Comet assay, Micronucleus

Project Number

15.FEN.BİL.44

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