Yara Örtü Materyallerinde Aktif Ajan Olarak Kullanılmak Üzere Jelatin Kaplı Gümüş Nanoparçacıkların Sentezlenmesi ve Karakterizasyonu

Year 2021, Volume: 21 Issue: 5, 1247 - 1258, 31.10.2021

Zehra Gün Gök

https://doi.org/10.35414/akufemubid.889646

Cited By: 1

Abstract

Bu çalışmada, yara örtü materyallerinde antimikrobiyal ajan olarak kullanılabilecek jelatin kaplı gümüş nanopartiküller (J-AgNPs) sentezlemek için jelatin proteini hem indirgeme hem de kaplama ajanı olarak kullanılmıştır. J-AgNPs sentezi için, %1’lik jelatin çözeltisi ile belirli derişimde AgNO3 çözeltisi karıştırılmış ve karışım 2 saat boyunca 100 °C’de inkübe edilmiştir. Jelatin çözeltisi eklenen gümüş nitrat çözeltilerinde gözlemlenen sarı renge-kahve renge dönüşüm, Ag(I) iyonunun Ag(0)’a indirgenmiş olduğunu göstermektedir. Bu renk değişimi olduktan sonra, karışım 8 saat boyunca oda sıcaklığında karıştırılmaya devam edilmiştir. J-AgNPs komplekslerinin 300 ile 600 nm arasındaki absorbans spektrası UV-Vis spektrofotometre ile ölçülerek gözlemlenen yüzey plazmon pikleri ile AgNPs oluşumu tespit edilmiştir. J-AgNPs’lerin sudaki kararlılıkları ve boyutları Zetasizer ile incelenmiştir. Sentezlenen bütün J-AgNPs gruplarının zeta potansiyeli pozitif çıkmış ve zeta-boyutlarının 116,3-170 nm aralığında olduğu bulunmuştur. Fourier dönüşümlü kızılötesi spektroskopisi (FTIR) analizleri ile J-AgNPs’lerin kimyasal yapısı incelenmiş ve AgNPs’lerin çevresinde jelatin bulunduğu gösterilmiştir. Yapılan taramalı elektron mikroskobu (SEM) incelemeleri ile sentezlenen J-AgNPs’lerin morfolojilerinin küresel formda olduğu anlaşılmış ve SEM görüntüleri üzerinden yapılan enerji dağılım spektrometresi (EDS) analizleri ile J-AgNPs’lerin yapısında karbon, azot ve gümüş varlığı kanıtlanmıştır. Sentezlenen J-AgNPs’lerin kristal yapısı ve termal özellikleri sırasıyla X-Işını difraktometresi (XRD) ve termogravimetrik analiz (TGA) yöntemleri ile incelenmiştir. XRD analizi ile jelatinin amorf yapısı ve J-AgNPs’lerde metalik gümüş varlığı gösterilmiştir. TGA analiz sonuçlarına göre, J-AgNPs’lerin termal olarak saf jelatine göre daha kararlı olduğu ve 900 °C’nin sonunda arta kalan miktarının gümüş varlığı ile arttığı görülmüştür. İleri çalışmalarla, J-AgNPs’leirn antimikrobiyal ve sitotoksik etkileri incelenecek ve yara örtü materyallerinde antimikrobiyal ajan olarak kullanılma kapasitesine sahip J-AgNPs grupları belirlenecektir.

Keywords

Jelatin, Gümüş nanopartikül, Yeşil sentez, Karakterizasyon, Antibakteriyel ajan

Supporting Institution

Kırıkkale Üniversitesi

Project Number

YOK

Thanks

Bu çalışma Kırıkkale Üniversitesi bünyesinde bulunan laboratuvarlarda gerçekleştirilmiştir. Desteklerinden dolayı Kırıkkale Üniversitesi teşekkür ederim.

Synthesis and Characterization of Gelatin Coated Silver Nanoparticles for Use as Active Agent in Wound Dressing Materials

Abstract

In this study, gelatin protein was used both as a reducing agent and as a coating agent to synthesize gelatin coated silver nanoparticles (J-AgNPs) that can be used as an antimicrobial agent in dressing materials. For the synthesis of J-AgNPs, 1% gelatin solution and AgNO3 solution of certain concentration were mixed and the mixture was incubated at 100 °C for 2 hours. The yellow color-brown transformation observed in silver nitrate solutions added with gelatin solution indicates that the Ag (I) ion has been reduced to Ag (0). After this color change occurred, the mixture was continued to stir for 8 hours at room temperature. By measuring the absorbance spectra of J-AgNPs complexes between 300 and 600 nm with UV-Vis spectrophotometer, AgNPs formation were detected with the observation of surface plasmon peaks. Stability and dimensions of J-AgNPs in water were examined with Zetasizer. The zeta potential of all synthesized J-AgNPs groups were found to be positive and zeta-dimensions were found to be in the range 116.3-170 nm. The chemical structure of J-AgNPs was examined by fouirer transform infrared spectrophotometer (FTIR) analysis and it was shown that there was gelatin around AgNPs. It was understood that the morphologies of J-AgNPs synthesized by scanning electron microscopy (SEM) examinations were in spherical form, and the presence of carbon, nitrogen and silver in the structure of J-AgNPs was proved by energy dispersion spectrometry (EDS) analyzes made on SEM images. Crystal structure and thermal properties of synthesized J-AgNPs were examined by X-Ray diffractometer (XRD) and thermogravimetric analysis (TGA) methods, respectively. The amorphous structure of gelatin and the presence of metallic silver in J-AgNPs were shown by XRD analysis. According to the TGA analysis results, it was observed that J-AgNPs were thermally more stable than pure gelatin and the remaining amount at the end of 900 °C increased with the presence of silver. With further studies, the antimicrobial and cytotoxic effects of J-AgNPs will be examined and the J-AgNPs groups capable of being used as antimicrobial agents in wound dressing materials will be determined.

Keywords

Gelatin, Silver nanoparticles, Green synthesis, Characterization, Antibacterial agent

Project Number

YOK

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