Investigation of Changes in Aquaporin 3 Protein in Van Fish (Alburnus tarichi) Gill, Kidney and Digestive Tract Tissues During Reproductive Migration

Year 2024, Volume: 24 Issue: 1, 8 - 13, 27.02.2024

Zehra Alkan , Ahmet Regaib Oğuz

https://doi.org/10.35414/akufemubid.1330373

Abstract

Lake Van is the largest lake in Turkey and also one of the few soda lakes in the world. Van fish (Alburnus tarichi) is the only vertebrate species that adapts to the physico-chemical (salinity ratio ‰22, pH 9.8) conditions of Lake Van. It is an anadromic fish and migrates from the lake to fresh waters between April and July. Fish that have completed their reproduction return to the lake environment. Some changes occur in tissues such as gills, kidneys and digestive tracts in order to adapt to fish that are exposed to different aquatic environments during migration. In this study, Aquaporin 3 transmembrane protein, which plays an important role in adaptation in the gill, kidney and digestive system of fish sampled from Lake Van and Karasu Stream, was examined immunohistochemically. As a result of the study, it was observed that the Aquaporin 3 transmembrane protein found in the gills was not marked in the fish sampled from the Karasu Stream. It was determined that Aquaporin 3 transmembrane protein in kidney tissue differed according to the sample area and its localization in the tissue. It was observed that Aquaporin 3 transmembrane protein found in the anterior and posterior parts of the intestine differed between the sampled localities and the labeling was in epithelial cells. It can be said that Aquaporin 3 transmembrane protein, which plays a role in water and glycerol transport, is effective in adaptation by showing changes in the osmoregulatory tissues of Van fish.

Keywords

Aquaporin, Digestive Tract, Gill, Kidney, Van Fish

Project Number

FDK-2021-9672

Van Balığı (Alburnus tarichi) Solungaç, Böbrek ve Sindirim Kanalı Dokularında Üreme Göçü Esnasında Akuaporin 3 Proteinindeki Değişimin İncelenmesi

Abstract

Van Gölü, Türkiye'nin en büyük gölü ve aynı zamanda dünyanın sayılı sodalı göllerinden biridir. Van balığı (Alburnus tarichi), Van Gölü'nün fiziko-kimyasal (tuzluluk oranı ‰22, pH 9.8) koşullarına uyum sağlayan tek omurgalı türüdür. Anadromik bir balık olup ve nisan-temmuz ayları arasında gölden tatlı sulara göç eder. Üremesini tamamlayan balıklar göl ortamına geri dönerler. Göç sırasında farklı su ortamlarına maruz kalan balıklarda adaptasyonu sağlamak için solungaç, böbrek, sindirim kanalı gibi dokularda bazı değişimler meydana gelir. Bu çalışmada Van Gölü ve Karasu Çayı'ndan örneklenen balıkların solungaç, böbrek ve sindirim sisteminde adaptasyonda önemli rol oynayan Akuaporin 3 transmembran proteini immünohistokimyasal olarak incelendi. Çalışma sonucunda solungaçlarda bulunan Akuaporin 3 transmembran proteininin Karasu Çayı'ndan örneklenen balıklarda işaretlenmediği görüldü. Böbrek dokusundaki Akuaporin 3 transmembran proteininin örnek alınan bölgeye ve dokudaki yerleşimine göre farklılık gösterdiği belirlendi. Bağırsağın ön ve arka kısımlarında bulunan Akuaporin 3 transmembran proteininin örneklenen lokaliteler arasında farklılık gösterdiği ve işaretlenmenin epitel hücrelerinde olduğu gözlendi. Su ve gliserol taşınmasında rol oynayan Akuaporin 3 transmembran proteinin Van balığı ozmoregülatör dokularında değişim göstererek adaptasyonda etkili olduğu söylenebilir.

Keywords

Akuaporin, Böbrek, Sindirim Kanlı, Solungaç, Van Balığı

Supporting Institution

VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ

Project Number

FDK-2021-9672

Thanks

Van YYÜ Bilimsel Araştırma Projeleri Koordinasyon Birimi

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