Effects of Intermittent Fasting Combined With Young Blood Plasma Administration on Aged Gut Microbiota

Year 2025, Volume: 25 Issue: 1, 1 - 9

Hüseyin Allahverdi , Burcu Baba

Abstract

The aging process leads to dysbiosis of the gut microbiota, which is associated with various diseases. In this context, the effects of intermittent fasting and young blood plasma transfusion on the renewal of senescent cells hold significant importance in the literature. The current study aims to evaluate the cumulative effects of these two interventions on the gut microbiota within the context of aging. The research was conducted on 24-month-old male Sprague-Dawley rats, examining the effects of a 30-day intermittent fasting protocol coupled with young blood plasma transfusion on the microbiota. Four distinct groups were defined: control (Cnt, n=7), those only undergoing intermittent fasting (Fst, n=7), those receiving only young plasma transfusion (Pls, n=7), and those undergoing both intermittent fasting and young plasma transfusion (FstPls, n=7). In the intermittent fasting regimen, rats were restricted from accessing food for 18 hours a day, followed by a 6-hour feeding window. For groups receiving young plasma transfusion, each animal was administered 0.5 ml of plasma daily. Metagenomic analysis results have shown significant inter-group differences in Shannon and Simpson alpha diversity indexes and the Firmicutes/Bacteroidetes ratio. However, no significant increase in species diversity was observed. Notably, the decrease in the F/B ratio following young plasma transfusion indicates a positive restructuring of the microbiota composition. These results contribute significantly to understanding the potential effects of interventions on the health of the aging gut microbiota, suggesting that optimizing the timing of these two approaches could offer synergistic benefits.

Keywords

Microbiota, Intermittent Fasting, Young Plasma, Metagenome, Sprague-Dawley

Aralıklı Oruç ile Birlikte Genç Kan Plazma Uygulamasının Yaşlı Bağırsak Mikrobiyotası Üzerindeki Etkileri

Abstract

Yaşlanma süreci, bağırsak mikrobiyotasının disbiyozisine ve bu durumun çeşitli hastalıklarla ilişkilendirilmesine neden olmaktadır. Bu kontekste, aralıklı oruç ve genç kan plazması transfüzyonunun, senesans hücrelerinin yenilenmesi üzerindeki etkileri literatürde önem arz etmektedir. Mevcut çalışma, yaşlanma bağlamında bu iki müdahalenin bağırsak mikrobiyotası üzerindeki kumulatif etkilerini değerlendirmeyi amaçlamaktadır. Araştırma, 24 aylık erkek Sprague-Dawley ratlar üzerinde gerçekleştirilmiş olup, 30 günlük bir aralıklı oruç protokolü esnasında genç kan plazması transfüzyonunun mikrobiyota üzerindeki etkilerini incelemiştir. Dört farklı grup tanımlanmıştır: kontrol (Cnt, n=7), yalnızca aralıklı oruç tutanlar (Fst, n=7), sadece genç plazma transfüzyonu alanlar (Pls, n=7), ve hem aralıklı oruç tutan hem de genç plazma transfüzyonu alanlar (FstPls, n=7). Aralıklı oruç rejiminde, ratlara günde 18 saat boyunca yiyecek erişimi kısıtlanmış, ardından 6 saatlik bir beslenme penceresi tanımlanmıştır. Genç plazma transfüzyonu uygulanan gruplarda, her bir hayvana günlük 0,5 ml plazma verilmiştir. Metagenomik analiz sonuçları, Shannon ve Simpson alfa çeşitlilik indeksleri ile Firmikutes/Bakterioidetes oranında anlamlı gruplar arası farklılıklar ortaya koymuştur. Ancak, tür çeşitliliği açısından anlamlı bir artış gözlemlenmemiştir. Özellikle, genç plazma transfüzyonu sonrası F/B oranındaki düşüş, mikrobiyota yapısında olumlu bir yeniden düzenlenmeye işaret etmektedir. Bu sonuçlar, yaşlanan bağırsak mikrobiyotasının sağlığı üzerindeki müdahalelerin potansiyel etkilerini derinlemesine anlamak için önemli bir katkı sağlamakta olup, bu iki yaklaşımın zamanlamasının optimizasyonunun, sinerjik faydalar sunabileceğini öne sürmektedir

Keywords

Mikrobiyota, Aralıklı Oruç, Genç Plazması, Metagenom, Sprague-Dawley

Ethical Statement

Çalışmamız Saki Yenilli Deney Hayvanı Üretim ve Uygulama Laboratuvarından Etik Kurul onayı (onay numarası: 2022/10) ile gerçekleştirilmiştir.

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