Pyridaben aktif maddesinin Ames Salmonella/Mikrozom Metodu ile Mutajenitesinin Değerlendirilmesi

Year 2025, Volume: 25 Issue: 4, 704 - 711, 04.08.2025

Dilek Akyıl

https://doi.org/10.35414/akufemubid.1592743

Abstract

Bu çalışma tarım alanında akarisit ve insektisit olarak kullanılan ve %20 pyridaben içeren Puzzle 20 WP’nin Ames/Salmonella mikrozom testi ile mutajenitesinin değerlendirilmesi olup çalışma S9 fraksiyon varlığı ve yokluğunda olmak üzere iki aşamalı olarak planlanmıştır. Deneyde Salmonella typhimurium’a ait en yaygın çalışılan iki suş olan TA100 ve TA98 mutant suşları kullanılmış olup uygulama öncesinde test maddelerinin sitotoksik dozları belirlenmiş ve deney için sitotoksik olmayan 5 farklı doz uygulaması yapılmıştır. Mutajenik aktiviteyi belirlemek amacıyla Puzzle 20 WP’nin 800, 400, 200, 100, 50 ve 25 μg/plak dozları çalışılmış olup bulgular Dunnett-t testi kullanılarak istatistiksel açıdan değerlendirilmiş ve veriler negatif kontrol değerleri ile kıyaslanarak değerlendirme yapılmıştır. Test bulgularına göre; Puzzle 20 WP’nin yalnızca 800 μg/plak dozunun hem S9 fraksiyonu varlığında hem de aynı dozun S9 fraksiyonu yokluğunda her iki suşta da mutajenik aktivite gösterdiği tespit edilmiştir

Keywords

Ames test, Akarisit, İnsektisit, Mutajenite

Assessment of Mutagenicity of Pyridaben by Ames Salmonella/Microsome Method

Abstract

This study is to evaluate the mutagenicity of Puzzle 20 WP (20% pyridaben), which is used as an insecticide and acaricide in agriculture, by Ames/Salmonella microsome test and the study was planned in two stages as in the presence and absence of S9 fraction. In the study, the two most widely studied strains of Salmonella typhimurium, TA98 and TA100 mutant strains, were used and the cytotoxic doses of the test substances were determined before the application and 5 different non-cytotoxic doses were applied for the experiment. In order to determine the mutagenic activity, Puzzle 20 WP doses of 800, 400, 200, 100, 50 and 25 μg/plate were applied and the data were evaluated statistically using Dunnett’s-t test and the data were compared with the negative control values. According to the test findings; it was determined that only 800 μg/plate dose of Puzzle 20 WP showed mutagenic activity in both strains both in the presence of S9 fraction and in the absence of S9 fraction at the same dose.

Keywords

Ames test;, Acaricide, İnsecticide, Mutagenicity

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