Yara Örtüsü Uygulamaları için Antimikrobiyal Peptid Konjuge PEGDA Tabanlı Hidrojeller

Year 2025, Volume: 25 Issue: 6, 1511 - 1520

Buse Sezer , Eda Bilgiç , Günnur Pulat

Abstract

Günümüzde, kronik yaraların bakımı önemli bir zorluk oluşturmaktadır. Kronik yaraların tedavisinde hidrojel bazlı yara örtüleri etkili bir yaklaşım olarak kabul edilmektedir. Poli(etilen glikol) diakrilat (PEGDA) hidrojeller, ayarlanabilir mekanik özellikler ve biyouyumluluk gibi özellikleri nedeniyle avantajlı bir hidrojel olarak kabul görmektedir. Ancak PEGDA hidrojelleri antimikrobiyal özelliklerden yoksundur. Bu nedenle, PEGDA hidrojellere antimikrobiyal özellikler kazanadırılması amacıyla yeni stratejilerin geliştirilmesi kronik enfekte yaralarla ilişkili sorunların üstesinden gelmek için kritik bir öneme sahiptir. Bu çalışmada, EDC/NHS kimyası kullanılarak antimikrobiyal peptit KSL-W'yi (KKVVFWVKFK) PEGDA hidrojeline konjuge ederek antimikrobiyal bir yara örtüsü geliştirilmiştir. Antimikrobiyal aktivite, planktonik formlarında MRSA ve çoklu ilaca dirençli (MDR) P. Aeruginosa üzerinde değerlendirilmiştir. Ayrıca, geliştirilen hidrojellerin sitotoksisitesi ve yara iyileşmesi üzerindeki etkinliği L929 fare fibroblast hücreleri kullanılarak değerlendirilmiştir. Geliştirilen KSL-W peptit konjuge PEGDA hidrojelleri, MRSA ve MDR P. aeruginosa'ya karşı sırasıyla 2,44 ve 1,15 log'luk inhibisyon ile güçlü antimikrobiyal aktivite göstermiştir. Ayrıca, geliştirilen hidrojeller sağlıklı memeli hücrelerine karşı istenmeyen toksik etki göstermemiş olup yara iyileşmesini desteklemiştir. Bulgularımız, biyomedikal uygulamalarda daha etkili fonksiyonel yara örtüleri geliştirmesi amacıyla antimikrobiyal hidrojellerin tasarımı açısından yol gösterici niteliktedir.

Keywords

Biyomalzemeler , yara örtüleri , hidrojeller , poli(etilen glikol) diakrilat , antimikrobiyal peptid

Antimicrobial Peptide Conjugated PEGDA Based Hydrogel for Wound Dressing Applications

Abstract

Currently, management of chronic wounds pose a significant challenge. In the treatment of chronic wounds, hydrogel based wound dressings considered as an effective approach. Poly(ethylene glycol) diacrylate (PEGDA) hydrogel is an advantageous hydrogel due to its properties such as tunable mechanical properties and biocompatibility. However, PEGDA hydrogels lack antimicrobial properties. Therefore, the development of new antibacterial strategies combined with PEGDA hydrogel can be effective in overcoming the problems associated with chronic infected wounds. Herein, we developed an antimicrobial wound dressing by conjugating antimicrobial peptide KSL-W (KKVVFWVKFK) to PEGDA hydrogel via EDC/NHS chemistry. The antimicrobial activity was evaluated against MRSA and multi-drug resistant (MDR) P. aeruginosa in their planktonic form. Furthermore, cytotoxicity and wound healing were assessed using L929 mouse fibroblast cells. Developed KSL-W peptide conjugated PEGDA hydrogels showed strong antimicrobial activity against MRSA and MDR P. aeruginosa with a bacterial reduction of 2.44 and 1.15 log, respectively. Furthermore, developed hydrogels showed no undesired toxic effect against healthy mammalian cells and supported wound healing. Our findings provide insight into the development of antimicrobial hydrogels for biomedical applications to develop more effective antimicrobial wound dressing solutions.

Keywords

Biomaterials , Wound healing dressings , Hydrogels , Poly(ethylene glycol) diacrylate , Antimicrobial peptide

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