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Abstract

In the study, the immunostimulant effects of the methanolic extract of nettle (Urtica dioica) on non-specific immune response of gold fish (Carassius auratus) were investigated. Two different concentration of extracts (0.1, 0.5 g/kg of feed) were individually mixed with the basal diet and fed to gold fish for 30 days. At the end of the study, blood samples were collected from caudal vein of the fish to assess the immunomodulatory characteristics of nettle using superoxide anion production (NBT), lysozyme, myeloperoxidase and phagocytic activity. In the study all immune parameters were affected positively by dietary nettle extract intake compared to control (P<0.05). The highest level of the nonspecific immune responses were determined in the 0.5% nettle group (P<0.05). According to study results, it can be say that nettle extract is an effective non-specific immunostimulant for gold fish

Keywords

• Gold fish, Methanolic extracts, Nettle, Immunostimulant

Isırgan otunun (Urtica dioica) metanolik özütünün japon balıklarının (Carassius auratus) doğal olmayan bağışıklık yanıtı üzerine etkileri

Abstract

Bu çalışmada ısırgan otunun metanolik özütünün japon balıklarının bağışıklık sistemi üzerine etkileri incelenmiştir. Özütün iki farklı konsantrasyonu (0,1 ve 0,5 g/kg yem) japon balığı yemi içerisine katılarak balıklar bu yemle 30 gün boyunca beslenmişlerdir. Çalışma sonunda balıkların kaudal venasından kan örneği alınarak süperoksit anyon üretimi (NBT), lizozim, myeloperoksidaz ve fagozitik aktivite gibi parametreler bağışıklık sisteminde meydan gelen değişimleri belirlemek için kullanılmıştır. Çalışma sonunda tüm bağışıklık yanıtların her iki ısırgan otu grubunda da kontrol grubu ile karşılaştırıldığında kayda değer yüksek olduğu belirlenmiştir (P < 0.05). Isırgan otu grupları kendi içerisinde değerlendirildiğinde en yüksek bağışıklık yanıtlar % 0,5 ısırgan otu içeren grupta gözlenmiştir. Çalışma sonuçlarına göre ısırgan otu özütünün japon balıkları için etkili bir bağışıklık uyarıcı olduğu söylenebilir.

Keywords

• Japon balığı, Metanolik özüt, Isırgan out, Bağışıklık Uyarıcı

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