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Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method

Year 2018, , 93 - 97, 30.06.2018

SELÇUK Kaplan

https://doi.org/10.28955/alinterizbd.402760
Cited By: 2

Abstract

Leptin is a hormone which produces 16 kDa protein predominantly expressed in adipose tissue. Leptin gene has pleiotropic effects on several functional traits such as milk yield, carcass and meat quality, growth, feed intake and fertility. Therefore, this study was performed to identify single nucleotide polymorphism of bubaline leptin gene in Anatolian buffaloes. In this study, 513 bp of leptin DNA fragment was amplified. A PCR-RFLP analysis was used to identify bubaline leptin gene T1131G polymorphism. DdeI restriction enzyme was used to genotype bubaline leptin gene T1131G polymorphism in Anatolian buffaloes. The analysis revealed TT, GT and GG genotypes in Anatolian buffaloes. Allele frequencies of T and G were found 0.478 and 0.521, respectively. This study is the first report of identification of bubaline leptin gene T1131G polymorphism by using PCR-RFLP method in Anatolian buffaloes.

Keywords

Leptin Anatolian Water Buffalo SNP PCR-RFLP

References

  • Adikari, M., 2006. Molecular characterization of leptin gene in murrah buffaloes. NDRI, Karnal.
  • Albarella, S., Ciotola, F., D’Anza, E., Coletta, A., Zicarelli, L., Peretti, V., 2017. Congenital Malformations in River Buffalo (Bubalus bubalis). Animals 7: 9.
  • Biolabs, N.E., Nebcutter V2. 0. Found at http://tools. neb. com/NEBcutter2/index. php.
  • Bondoc, O.L., 2013. Genetic Diversity and Relationship of Domestic Buffalo and Cattle Breeds and Crossbreeds (Subfamily Bovinae) in the Philippines Based on the Cytochrome C Oxidase I (COI) Gene Sequence. The Philippine Agricultural Scientist 96.
  • Borghese, A., Mazzi, M., 2005. Buffalo population and strategies in the world. Buffalo production and research 67: 1-39.
  • Buchanan, F., Van Kessel, A., Waldner, C., Christensen, D., Laarveld, B., Schmutz, S., 2003. Hot topic: an association between a leptin single nucleotide polymorphism and milk and protein yield. Journal of Dairy Science 86: 3164-3166.
  • Cho, S., Park, T.-S., Yoon, D.-H., Cheong, H.-S., Namgoong, S., Park, B.-L., Lee, H.-W., Han, C.-S., Kim, E.-M., Cheong, I.-C., 2008. Identification of genetic polymorphisms in FABP3 and FABP4 and putative association with back fat thickness in Korean native cattle. BMB reports 41: 29-34.
  • Clempson, A., Pollott, G., Brickell, J., Bourne, N., Munce, N., Wathes, D., 2011. Evidence that leptin genotype is associated with fertility, growth, and milk production in Holstein cows. Journal of dairy science 94: 3618-3628.
  • Cockrill, W.R., 1981. The water buffalo: a review. British Veterinary Journal 137: 8-16.
  • Datta, S., Adikari, A., Chauhan, A., Verma, A., Gupta, I., Chauhan, I., Azad, M.S., HM, Y., 2012. Nucleotide sequence variation in leptin gene of Murrah buffalo (Bubalus bubalis). Exper Anim Med Res 2: 130-136.
  • De la Rosa Reyna, X., Montoya, H., Castrellón, V., Rincón, A., Bracamonte, M., Vera, W., 2010. Polymorphisms in the IGF1 gene and their effect on growth traits in Mexican beef cattle. Genet Mol Res 9: 875-883.
  • Dekkers, J.C., 2002. Multifactorial genetics: The use of molecular genetics in the improvement of agricultural populations. Nature Reviews Genetics 3: 22.
  • Frühbeck, G., 2001. A heliocentric view of leptin. Proceedings of the Nutrition Society 60: 301-318.
  • Grisart, B., Coppieters, W., Farnir, F., Karim, L., Ford, C., Berzi, P., Cambisano, N., Mni, M., Reid, S., Simon, P., 2002. Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome research 12: 222-231.
  • Grobet, L., Martin, L.J.R., Poncelet, D., Pirottin, D., Brouwers, B., Riquet, J., Schoeberlein, A., Dunner, S., Ménissier, F., Massabanda, J., 1997. A deletion in the bovine myostatin gene causes the double–muscled phenotype in cattle. Nature genetics 17: 71.
  • Haegeman, A., Van Zeveren, A., Peelman, L., 2000. New mutation in exon 2 of the bovine leptin gene. Animal Genetics 31: 79-79.
  • Høj, S., Fredholm, M., Larsen, N., Nielsen, V., 1993. Growth hormone gene polymorphism associated with selection for milk fat production in lines of cattle. Animal Genetics 24: 91-96.
  • Jhala, N., Rank, D., Vataliya, P., Joshi, C., Bhong, C., Mehta, H., Patil, A., 2009. Cloning and sequencing of the leptin gene in Gir cattle and Mehsana buffalo. Buffalo Bulletin 28: 29-33.
  • Komisarek, J., Szyda, J., Michalak, A., Dorynek, Z., 2005. Impact of leptin gene polymorphisms on breeding value for milk production traits in cattle. Journal of Animal and Feed Sciences 14: 491.
  • Konfortov, B.A., Licence, V.E., Miller, J.R., 1999. Re-sequencing of DNA from a diverse panel of cattle reveals a high level of polymorphism in both intron and exon. Mammalian Genome 10: 1142-1145.
  • Kulig, H., Kmieć, M., 2009. Association between leptin gene polymorphisms and growth traits in Limousin cattle. Russian journal of genetics 45: 738-741.
  • Lagonigro, R., Wiener, P., Pilla, F., Woolliams, J., Williams, J., 2003. A new mutation in the coding region of the bovine leptin gene associated with feed intake. Animal Genetics 34: 371-374.
  • Liefers, S.C., Te Pas, M.F., Veerkamp, R.F., Chilliard, Y., Delavaud, C., Gerritsen, R., van der Lende, T., 2003. Association of leptin gene polymorphisms with serum leptin concentration in dairy cows. Mammalian Genome 14: 657-663.
  • Naqvi, A., 2007. Application of molecular genetic technologies in livestock production: potentials for developing countries. Advances in Biological Research 1: 72-84.
  • Orrù, L., Terzano, G., Napolitano, F., Savarese, M., De Matteis, G., Scatä, M., Catillo, G., Moioli, B., 2007. DNA polymorphisms in river buffalo leptin gene. Italian Journal of Animal Science 6: 342-344.
  • Scatà, M., Grandoni, F., Antonelli, S., De Matteis, G., Napolitano, F., 2012. Single nucleotide polymorphisms detected and in silico analysis of the 5’flanking sequence and exon 1 in the Bubalus bubalis leptin gene. Journal of Buffalo Science 1: 73-77.
  • Schenkel, F., Miller, S., Jiang, Z., Mandell, I., Ye, X., Li, H., Wilton, J., 2006. Association of a single nucleotide polymorphism in the calpastatin gene with carcass and meat quality traits of beef cattle 1. Journal of Animal Science 84: 291-299.
  • Schenkel, F., Miller, S., Ye, X., Moore, S., Nkrumah, J., Li, C., Yu, J., Mandell, I., Wilton, J., Williams, J., 2005. Association of single nucleotide polymorphisms in the leptin gene with carcass and meat quality traits of beef cattle 1. Journal of Animal Science 83: 2009-2020.
  • Scherf, B.D., 2000. World watch list for domestic animal diversity. Food and Agriculture Organization (FAO).
  • Soysal, M., Ozkan, E., Kok, S., Occidente, M., Tuna, Y., Gurcan, E., Matassino, D., 2007. Genetic characterization of indigenous anatolian water buffalo breed using microsatellite dna markers. Italian Journal of Animal Science 6: 409-412.
  • Tanpure, T., Dubey, P.K., Singh, K.P., Kathiravan, P., Mishra, B.P., Niranjan, S.K., Kataria, R.S., 2012. PCR-SSCP analysis of leptin gene and its association with milk production traits in river buffalo (Bubalus bubalis). Tropical animal health and production 44: 1587-1592.
  • Vallinoto, M., Schneider, M., Silva, A., Iannuzzi, L., Brenig, B., 2004. Molecular cloning and analysis of the swamp and river buffalo leptin gene. Animal genetics 35: 462-463.
  • Yeh, F., Yang, R., Boyle, T., 1999. Popgene version 1.32: Microsoft Windows-based freeware for population genetic analysis. University of Alberta, Edmonton.
  • Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L., Friedman, J.M., 1994. Positional cloning of the mouse obese gene and its human homologue. Nature 372: 425.

Year 2018, , 93 - 97, 30.06.2018

SELÇUK Kaplan

https://doi.org/10.28955/alinterizbd.402760
Cited By: 2

Abstract

PCR-RFLP Metodu Kullanılarak Manda Leptin Geni Polimorfizminin Anadolu Mandalarında Karakterizasyonu

Leptin yağ dokusunda ağırlıklı olarak ifade edilen 16 kDa protein üreten bir hormondur. Leptin geni, süt verimi, karkas ve et kalitesi, büyüme, yem tüketimi ve üreme gibi çeşitli fonksiyonel özellikler üzerine pleotropik etkilere sahiptir. Bu nedenle, bu araştırma Anadolu mandalarındaki manda leptin geninde tek nükleotid polimorfizmini belirlemek amacıyla yapılmıştır. Çalışmada, Leptin geninde 513 baz çiftlik bir bölge çoğaltılmıştır. 513 baz çiftlik fragment içerisinde T1131G polimorfizmini belirlemek üzere PCR-RFLP yöntemi kullanılmıştır. Anadolu mandalarında manda leptin geni T1131G polimorfizminin belirlenmesi için DdeI restriksiyon enzimi kullanılmıştır. Yapılan PCR-RFLP analizi sonucunda ele alınan Anadolu mandalarında TT, GT ve GG genotipleri belirlenmiş ve T ve G allel frekansları sırasıyla 0.478 ve 0.521 olarak bulunmuştur. Bu çalışma, Anadolu mandalarında PCR-RFLP yöntemi ile manda leptin geni T1131G polimorfizminin tanımlanmasına ilişkin ilk çalışma sonuçlarıdır.

Keywords

Leptin Anadolu su mandası SNP PCR-RFLP

References

  • Adikari, M., 2006. Molecular characterization of leptin gene in murrah buffaloes. NDRI, Karnal.
  • Albarella, S., Ciotola, F., D’Anza, E., Coletta, A., Zicarelli, L., Peretti, V., 2017. Congenital Malformations in River Buffalo (Bubalus bubalis). Animals 7: 9.
  • Biolabs, N.E., Nebcutter V2. 0. Found at http://tools. neb. com/NEBcutter2/index. php.
  • Bondoc, O.L., 2013. Genetic Diversity and Relationship of Domestic Buffalo and Cattle Breeds and Crossbreeds (Subfamily Bovinae) in the Philippines Based on the Cytochrome C Oxidase I (COI) Gene Sequence. The Philippine Agricultural Scientist 96.
  • Borghese, A., Mazzi, M., 2005. Buffalo population and strategies in the world. Buffalo production and research 67: 1-39.
  • Buchanan, F., Van Kessel, A., Waldner, C., Christensen, D., Laarveld, B., Schmutz, S., 2003. Hot topic: an association between a leptin single nucleotide polymorphism and milk and protein yield. Journal of Dairy Science 86: 3164-3166.
  • Cho, S., Park, T.-S., Yoon, D.-H., Cheong, H.-S., Namgoong, S., Park, B.-L., Lee, H.-W., Han, C.-S., Kim, E.-M., Cheong, I.-C., 2008. Identification of genetic polymorphisms in FABP3 and FABP4 and putative association with back fat thickness in Korean native cattle. BMB reports 41: 29-34.
  • Clempson, A., Pollott, G., Brickell, J., Bourne, N., Munce, N., Wathes, D., 2011. Evidence that leptin genotype is associated with fertility, growth, and milk production in Holstein cows. Journal of dairy science 94: 3618-3628.
  • Cockrill, W.R., 1981. The water buffalo: a review. British Veterinary Journal 137: 8-16.
  • Datta, S., Adikari, A., Chauhan, A., Verma, A., Gupta, I., Chauhan, I., Azad, M.S., HM, Y., 2012. Nucleotide sequence variation in leptin gene of Murrah buffalo (Bubalus bubalis). Exper Anim Med Res 2: 130-136.
  • De la Rosa Reyna, X., Montoya, H., Castrellón, V., Rincón, A., Bracamonte, M., Vera, W., 2010. Polymorphisms in the IGF1 gene and their effect on growth traits in Mexican beef cattle. Genet Mol Res 9: 875-883.
  • Dekkers, J.C., 2002. Multifactorial genetics: The use of molecular genetics in the improvement of agricultural populations. Nature Reviews Genetics 3: 22.
  • Frühbeck, G., 2001. A heliocentric view of leptin. Proceedings of the Nutrition Society 60: 301-318.
  • Grisart, B., Coppieters, W., Farnir, F., Karim, L., Ford, C., Berzi, P., Cambisano, N., Mni, M., Reid, S., Simon, P., 2002. Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome research 12: 222-231.
  • Grobet, L., Martin, L.J.R., Poncelet, D., Pirottin, D., Brouwers, B., Riquet, J., Schoeberlein, A., Dunner, S., Ménissier, F., Massabanda, J., 1997. A deletion in the bovine myostatin gene causes the double–muscled phenotype in cattle. Nature genetics 17: 71.
  • Haegeman, A., Van Zeveren, A., Peelman, L., 2000. New mutation in exon 2 of the bovine leptin gene. Animal Genetics 31: 79-79.
  • Høj, S., Fredholm, M., Larsen, N., Nielsen, V., 1993. Growth hormone gene polymorphism associated with selection for milk fat production in lines of cattle. Animal Genetics 24: 91-96.
  • Jhala, N., Rank, D., Vataliya, P., Joshi, C., Bhong, C., Mehta, H., Patil, A., 2009. Cloning and sequencing of the leptin gene in Gir cattle and Mehsana buffalo. Buffalo Bulletin 28: 29-33.
  • Komisarek, J., Szyda, J., Michalak, A., Dorynek, Z., 2005. Impact of leptin gene polymorphisms on breeding value for milk production traits in cattle. Journal of Animal and Feed Sciences 14: 491.
  • Konfortov, B.A., Licence, V.E., Miller, J.R., 1999. Re-sequencing of DNA from a diverse panel of cattle reveals a high level of polymorphism in both intron and exon. Mammalian Genome 10: 1142-1145.
  • Kulig, H., Kmieć, M., 2009. Association between leptin gene polymorphisms and growth traits in Limousin cattle. Russian journal of genetics 45: 738-741.
  • Lagonigro, R., Wiener, P., Pilla, F., Woolliams, J., Williams, J., 2003. A new mutation in the coding region of the bovine leptin gene associated with feed intake. Animal Genetics 34: 371-374.
  • Liefers, S.C., Te Pas, M.F., Veerkamp, R.F., Chilliard, Y., Delavaud, C., Gerritsen, R., van der Lende, T., 2003. Association of leptin gene polymorphisms with serum leptin concentration in dairy cows. Mammalian Genome 14: 657-663.
  • Naqvi, A., 2007. Application of molecular genetic technologies in livestock production: potentials for developing countries. Advances in Biological Research 1: 72-84.
  • Orrù, L., Terzano, G., Napolitano, F., Savarese, M., De Matteis, G., Scatä, M., Catillo, G., Moioli, B., 2007. DNA polymorphisms in river buffalo leptin gene. Italian Journal of Animal Science 6: 342-344.
  • Scatà, M., Grandoni, F., Antonelli, S., De Matteis, G., Napolitano, F., 2012. Single nucleotide polymorphisms detected and in silico analysis of the 5’flanking sequence and exon 1 in the Bubalus bubalis leptin gene. Journal of Buffalo Science 1: 73-77.
  • Schenkel, F., Miller, S., Jiang, Z., Mandell, I., Ye, X., Li, H., Wilton, J., 2006. Association of a single nucleotide polymorphism in the calpastatin gene with carcass and meat quality traits of beef cattle 1. Journal of Animal Science 84: 291-299.
  • Schenkel, F., Miller, S., Ye, X., Moore, S., Nkrumah, J., Li, C., Yu, J., Mandell, I., Wilton, J., Williams, J., 2005. Association of single nucleotide polymorphisms in the leptin gene with carcass and meat quality traits of beef cattle 1. Journal of Animal Science 83: 2009-2020.
  • Scherf, B.D., 2000. World watch list for domestic animal diversity. Food and Agriculture Organization (FAO).
  • Soysal, M., Ozkan, E., Kok, S., Occidente, M., Tuna, Y., Gurcan, E., Matassino, D., 2007. Genetic characterization of indigenous anatolian water buffalo breed using microsatellite dna markers. Italian Journal of Animal Science 6: 409-412.
  • Tanpure, T., Dubey, P.K., Singh, K.P., Kathiravan, P., Mishra, B.P., Niranjan, S.K., Kataria, R.S., 2012. PCR-SSCP analysis of leptin gene and its association with milk production traits in river buffalo (Bubalus bubalis). Tropical animal health and production 44: 1587-1592.
  • Vallinoto, M., Schneider, M., Silva, A., Iannuzzi, L., Brenig, B., 2004. Molecular cloning and analysis of the swamp and river buffalo leptin gene. Animal genetics 35: 462-463.
  • Yeh, F., Yang, R., Boyle, T., 1999. Popgene version 1.32: Microsoft Windows-based freeware for population genetic analysis. University of Alberta, Edmonton.
  • Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L., Friedman, J.M., 1994. Positional cloning of the mouse obese gene and its human homologue. Nature 372: 425.
There are 34 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

SELÇUK Kaplan

Publication Date June 30, 2018
Acceptance Date March 26, 2018
Published in Issue Year 2018

Cite

APA Kaplan, S. (2018). Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method. Alinteri Journal of Agriculture Science, 33(1), 93-97. https://doi.org/10.28955/alinterizbd.402760
AMA Kaplan S. Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method. Alinteri Journal of Agriculture Science. June 2018;33(1):93-97. doi:10.28955/alinterizbd.402760
Chicago Kaplan, SELÇUK. “Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method”. Alinteri Journal of Agriculture Science 33, no. 1 (June 2018): 93-97. https://doi.org/10.28955/alinterizbd.402760.
EndNote Kaplan S (June 1, 2018) Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method. Alinteri Journal of Agriculture Science 33 1 93–97.
IEEE S. Kaplan, “Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method”, Alinteri Journal of Agriculture Science, vol. 33, no. 1, pp. 93–97, 2018, doi: 10.28955/alinterizbd.402760.
ISNAD Kaplan, SELÇUK. “Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method”. Alinteri Journal of Agriculture Science 33/1 (June 2018), 93-97. https://doi.org/10.28955/alinterizbd.402760.
JAMA Kaplan S. Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method. Alinteri Journal of Agriculture Science. 2018;33:93–97.
MLA Kaplan, SELÇUK. “Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method”. Alinteri Journal of Agriculture Science, vol. 33, no. 1, 2018, pp. 93-97, doi:10.28955/alinterizbd.402760.
Vancouver Kaplan S. Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method. Alinteri Journal of Agriculture Science. 2018;33(1):93-7.

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