Determination of Some Biological Control Agents Against Alternaria Fruit Rot in Quince

Year 2019, , 25 - 31, 30.06.2019

Nasibe Tekiner Recep Kotan Elif Tozlu Fatih Dadaşoğlu

https://doi.org/10.28955/alinterizbd.578541

Cited By: 6

Abstract

The use of bioagents has become important as an alternative to
fungicides to prevent postharvest losses in recent years. In this context, it
is aimed to investigate effective of some bacterial and fungal biocontrol
agents for control against Alternari
alternata, which has a wide range of hosts, leading to losses pre and
postharvest. In this aim, dual culture of nine bacterial bio-agents isolate [Bacillus megaterium (TV 3D), Bacillus
subtilis (TV
6F, TV 17C, CP 1), Bacillus cereus
(TV 85D), Paenibacillus polymxa (TV
12E), Pantoea agglomerans (RK 79, RK
92), Pseudomonas fluorescens (MF 3)]
and 3 fungal bioagents [Trichoderma
harzianum (ET 4, ET 14, NT 1)] were tested for antagonistic properties
against Alternari alternata under in vitro conditions. It has been determined
that all bio-agents have an inhibitory effect on
the growth of pathogen fungus under in
vitro conditions. RK 79 (79.76%) was the most effective isolate in bio-agent bacteria isolates. All of the bio-agent fungal isolates showed a high hyperparasitic
effect and the most effective isolate was ET 4 (67.74%). Consequently,
promising results were obtained from these bio-agent bacteria
and fungi.  It is important to carry out
studies in vivo bioassays in order to
control postharvest decay with bacterial and fungal bio-agents
which are determined to be effective. 

Keywords

Biological control, bio-agent bacteria, bio-agent fungi, Bacilus, Trichoderma

References

• Abbo, A. S., Idris, M. O. and Hammad A. M. 2014. The antifungal effects of four tomato rhizosphere Bacillus spp. against Alternaria alternata. International Journal of Science and Research (IJSR), 3(7): 1324-1328.
• Acarsoy, N. and Mısırlı, A. 2010. Kayısıda monilyaya dayanıklılık ıslahı. Ege Üniversity Journal of Agriculture, 47 (3):1018-8851.
• Agrios, G. N., 1997. Plant Pathology. Academic Press, New York, USA.
• Ahmed I. S., 2017. Biological control of potato brown leaf spot disease caused by Alternaria alternata using Brevibacillus formosus strain DSM 9885 and Brevibacillus brevis strain NBRC 15304. Journal of Plant Pathology and Microbiology, 8(6):1-8.
• Arzanlou, M., Khodaei, S., Narmani, A., Ahari, A. and Motallebi A. 2014. Inhibitory effect of Trichoderma isolates on growth of Alternaria alternata, the causal agent of leaf spot disease on sunflower, under laboratory conditions. Archives of Phytopathology and Plant Protection, 47(13):1592-1599.
• Beer, S., Rundle, J.R. and Norelli, J.L. 1984. Recent progress in the development of biological control of fire blight—a review. Acta Horticulture, 151:195–201.
• Begum, M. F., Rahman, M. A. and. Alam, M. F. 2010. Biological control of Alternaria fruit rot of chili by Trichoderma species under field conditions. Mycobiology, 38(2): 113-117.
• Büyükyılmaz, M. 1999. Ayva çeşit seçimi. Atatürk Bahçe Kültürleri Merkez Araştırma Enstitüsü, Proje Kod No: TAGEM /96/06/003, Yayın No:125.
• Chernin, L., Ismailov, Z., Haran, S. and Chet, I. 1995. Chitinolytic Enterobacter agglomerans antagonistic to fungal plant pathogens. Application Environmental Microbiology, 61(5): 1720-1726.
• Chet, I. 1987. Trichoderma application, mode of action, and potential as a biocontrol agent of soilborne plant pathogenic fungi. In: Chet I, Editor, Innovative Approaches to Plant Disease Control, Wiley, NewYork, 137-160.
• Droby, S. 2006. Improving quality and safety of fresh fruit and vegetables after harvest by the use of biocontrol agents and natural materials. Acta Horticulturae, 709: 45–51.
• Ekinci M., Turan M., Yıldırım E., Güneş A., Kotan R. and Dursun A. 2014. Effect of plant growth promoting rhizobacteria on growth, nutrient, organic acid, amino acid and hormone content of cauliflower (Brassica oleracea L. var. botrytis) transplants. Acta Scientiarum Polonorum, 13(6): 71–85.
• Ekinci, M., Yıldırım, E. and Kotan, R. 2015. Effects of different plant growth promoting rhizobacteria on growth and quality of broccoli (Brassica oleraceae L. var. italica) seedling. Akdeniz University Journal of Agriculture, 28(2): 53-59.
• El-Ghaouth, A., Wilson, C.L. and Wisniewski, M.E. 2004. Biologically based alternatives to synthetic fungicides for the post harvest diseases of fruit and vegetables. In: Naqvi, S.A.M.H. (Ed.), Diseases of Fruit and Vegetables, Kluwer Academic Publishers, 2: 511–535.
• Erman, M., Kotan, R., Çakmakçı, R., Çığ, F., Karagöz, K. and Sezen, M. 2010. Effect of nitrogen fixing and phosphate-solubilizing rhizobacteria isolated from Van Lake Basin on the growth and quality properties in wheat and sugar beet. Turkey IV. Organic Farming Symposium, 28 June - 1 July 2010, Erzurum, Turkey, pp: 325-329.
• Francés, J., Bonaterra, A., Moreno, M.C., Cabrefiga, J., Badosa, E. and Montesinos, E. 2006. Pathogen aggressiveness and post harvest biocontrol efficiency in Pantoea agglomerans. Postharvest Biology and Technology, 39, 299–307.
• Harman, G. 1993. Chitinolytic Enzymes of Trichoderma harzianum: Purification of chitobiosidase and endochitinase. Phytopathology, 83(3):313.
• Ghosh, R., Barman, S., Khatun, J. and Mandal, N. C. 2016. Biological control of Alternaria alternata causing leaf spot disease of Aloe vera using two strains of rhizobacteria. Biological control, 97: 7-108
• Goldman, M. H. and Goldman, G.H. 1998. Trichoderma harzianum transformant has high extracellular alkaling proteinase expression during specific mycoparasitic interaction. Genetics and Molecular Biology, 21:15-18.
• Güneş, A., Karagöz, K., Turan, M., Kotan, R., Yıldırım, E., Çakmakçı, R. and Şahin F. 2015. Fertilizer effiency of some plant growth promoting rhizobacteria for plant growth. Research Journal of Soil Biology, 7(2): 28-45.
• Jayapradha, C. and Yesu, R.Y.I. 2016. A review of eco-friendly management of Alternaria species. Indian Association of Hill Farming, 29:1-14.
• Karakurt, H., Kotan, R., Aslantaş, R., Dadaşoğlu, F., Karagöz, K., Şahin, F. 2010. Bitki büyümesini teşvik eden bazı bakteri strainlerinin ‘şekerpare’ kayısı çöğürlerinin bitki gelişimi üzerine etkileri. Journal of Agricultural Faculty of Atatürk University, 41(1): 7-12.
• Kearns, L.P. and Hale, C.N., 1995. Incidence of bacteria inhibitory to Erwinia amylovora from blossoms in New Zealand apple orchards. Plant Pathology, 44: 918–924
• Kotan, R., Sahin, F., Demirci, E., Özbek, A., Eken, C. and Miller, S.A. 1999. Evaluation of antagonistic bacteria for biological control of Fusarium dry rot of potato. Phytopathology, 89(6): 41.
• Kotan, R., Sahin, F., Demirci, E. and Eken, C. 2002. Studies on the biological control of potato dry rot disease caused by Fusarium solani with application of some bacterial strains. In: 5th Biological Control Congres, 4-7 September 2002. Erzurum, Turkey, pp. 381–390.
• Kotan, R., Şahin F., Demirci E., and Eken C. 2009. Biological control of the potato dry rot caused by Fusarium species using PGPR strains. Biological Control, 50:194–198.
• Mari, M., Iori, R., Leoni, O. and Marchi, A. 1993. In vitro activitiy of glucosinolate-derived isothiocyanates against postharvest fruit pathogens. Annals of Applied Biology, 123: 155–164.
• Mari, M., Guizzardi, M. and Pratella, G. C. 1996. Biological control of gray mould in pears by antagonistic bacteria. Biological Control, 7: 30–7.
• Mari, M. and Guizzardi, M. 1998. The post harvest phase: emerging technologies for the control of fungal diseases. Phytoparasitica, 26(1): 59–66.
• Mari, M., Bertolini, P. and Pratella, G. 2003. Non-conventional methods for the control of post harvest pear diseases. Journal of Applied Microbiology, 94(5): 761–766.
• Moller, E. M., Bahnweg, G., Sandermann, H. and Geiger, H. H. 1992. A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies and infected plant tissues. Nucleic Acids Research, 20(22): 6115–6116.
• Montesinos, E., Bonaterra, A., Ophir, Y. and Beer, S. 1996. Antagonism of selected bacterial strains to Stemphylium vesicarium and biological control of brown spot of pear under controlled environment conditions. Phytopathology, 86:856–863.
• Monte, E. 2001. Understanding Trichoderma: between biotechnology and microbial ecology. International Journal of Microbiology, 4:1-4.
• Pane, C. and Zaccardelli, M. 2015. Evaluation of Bacillus strains isolated from Solanaceous phylloplane for biocontrol of Alternaria early blight of tomato. Biological Control, 84: 11-18.
• Pandey, A., 2010. Antagonism of two Trichoderma species against Alternaria alternata on Capsicum frutescens. Journal of Experimental Sciences, 1(5):18-19.
• Panwar, V., Gangwar, R. K., S. Javeria, S. and Yadav, R.S. 2013. Antifungal efficacy of fungicides and bio-control agents against leaf spot pathogens, Alternaria alternata. Current Discovery, 2 (2): 128-133.
• Pastor, N., Carlier, E., Andrés, J., Rosas, S. B., Rovera, M. 2012. Characterization of rhizosphere bacteria for control of phytopathogenic fungi of tomato. Journal of Environmental Management, 95: 332-337.
• Roco, A. and Perez L. M. 2001In vitro biocontrol activity of Trichoderma harzianum on Alternaria alternata in the presence of growth regulators. Electronic Journal of Biotechnology, 4(2): 68–73.
• Sadfi, N., Chérif, M., Hajlaoui, M.R. and Boudabbous, A. 2002. Biological control of the potato tubers dry rot caused by Fusarium roseum var. sambucinum under greenhouse, field and storage conditions using Bacillus spp. isolates. Journal of Phytopathology, 150 (11,12): 640–648.
• Schisler, D.A., Slininger, P.J. and Bothast, R.J. 1997. Effects of antagonists cell concentration and two-strain mixtures on biological control of Fusarium dry rot of potatoes. Phytopathology, 87: 177–183.
• Sempere, F. and Santamarina, M. P. 2007. In vitro biocontrol analysis of Alternaria alternata (Fr.) Keissler under different environmental conditions. Mycopathologia, 163:183–190.
• Singh, D. and Sharma, R.R. 2007. Post harvest diseases of fruit and vegetables and their management. In: Prasad, D. (Ed.), Sustainable Pest Management. Daya Publishing House, New Delhi, India.
• Skidmore, A. M. and Dickinson, C. M. 1976. Colony interactions and hyphal interference between Septoria nodorum and Phylloplane fungi. Transactions of the British Mycological Society, 66: 57–64.
• Şirikçi, B.S. and Gül, M. 2017. Türkiye ve Dünyada Ayva Piyasası. Türk Tarım – Gıda Bilim ve Teknoloji Dergisi, 5(6): 600-606.
• Tekiner, N., Tozlu, E., Kotan, R. and Dadasoğlu, F. 2018. Biological control of Botrytis cinerea and Alternaria alternata with bioagent bacteria and fungi under in vitro conditions. Conference: 2nd International professional and technical sciences congress (UMTEB) May 10-13, 2018 Batumi / Georgia.
• Tozlu, E. 2016. Bazı bakteriyel biyokontrol ajanlar ile havuç acı çürüklük hastalığı (Geotrichum candidum Link)’nın Biyolojik Mücadelesi. Atatürk University Journal of the Agricultural Faculty, 47 (1): 1-9.
• Tozlu, E., Tekiner, N. and Kotan, R. 2018a. Screening of Trichoderma harzianum Rifai (1969) isolates of domestic plant origin against different fungal plant pathogens for use as biopesticide. Fresenius Environmental Bulletin, 27(6): 4232-4238.
• Tozlu, E., Tekiner, N., Kotan, R. and Örtücü, S. 2018b. Investigation on the biological control of Alternaria alternata. Indian Journal of Agricultural Sciences, 88 (8): 1241-1247.
• Tozlu E., Tekiner N., Tozlu G., Kotan R., Çalmaşur, Ö., Göktürk, T. and Dadaşoğlu, F. 2018c. Icerya purchasi Maskell, 1878 (Hemiptera: Margarodidae)'nin Entomopatojen Fungus ve Bakterilerle Biyolojik Mücadelesinin Araştırılması. Conference: III. Türkiye Orman Entomolojisi ve Patolojisi Sempozyumu, 10-12 Mayıs 2018, Artvin, Türkiye.
• Troncoso-Rojas, R. and Tiznado-Hernández, M. E. 2014, Alternaria alternata (Black Rot, Black Spot). Science Direct, Post harvest Decay book, 147-187.
• Usall, J., Teixido, N., Nunes, C. and Vinas, I. 2001. Biological control of postharvest pear diseases using a bacterium, Pantoea agglomerans CPA-2.), International Journal of Food Microbiology, 70: 53–61.
• Ulhoa C.J. and Peberdy J.F. 1991. Regulation of chitinase synthesis in Trichoderma harzianum. Journal of General Microbiology, 137(9): 2163-2169.
• Voland, R.P., Johnson, T.E. and Mcmanus, P.S., 1999. Inhibition of Monilinia oxycocci by bacteria isolated from a cranberry marsh. Biocontrol, 44: 473–485.
• Wan, Y. and Tian, S. 2015. Integrated control of post harvest diseases of pear fruits using antagonistic yeasts in combination with ammonium molybdate. Journal of the Science of Food and Agriculture, 85(15): 2605–2610.
• Wilson, C. L. and Pusey, P.L. 1985. Potential for biological control of post harvest plant diseases. Plant Disease, 69(5): 375–378.
• Wright, S. A., Zumoff, C.H., Schneider, L. and Beer, S. V. 2001. Pantoea agglomerans strain Eh318 produces two antibiotics that inhibit Erwinia amylovora in vitro. Applied and Environmental Microbiology, 67: 284-292.
• Zhang, X., Zhang, Y., Zhang, Z., Zhang, S, Han, J. and Liu, H. 2014. Identification of Pantoea agglomerans XM2 with biocontrol activity against postharvest pear black spot. Wei Sheng Wu Xue Bao, 54(6): 648–655.