Research Article
Zeytin Yaprağının (Olea Europaea L.) Diyabetik Sıçanlarda Bazı Biyokimyasal ve Hematolojik Parametreler Üzerine Etkisi
Abstract
Bu çalışma, değişik oranlardaki zeytin yaprağı ekstraktının (Olive Leaf
Extract-OLE) ve infüzyonunun streptozosin ile oluşturulmuş deneysel diyabetik
sıçanların bazı biyokimyasal ve hematolojik parametreleri üzerine olası
koruyucu etkisini araştırmak amacıyla planlanmıştır. Bu amaç doğrultusunda, 56
adet Wistar albino sıçan rastgele 7
gruba ayrıldı (n=8). 21
günlük deneme sonunda biyokimyasal ve hematolojik analizleri yapıldı. OLE
uygulanan grupların hepsinde RBC’ler istatiksel anlamda etkilenmezken, HBG,
HCT, MCV’nin pozitif yönde etkilendiği görüldü (p<0.05). Diyabet+OLE-50 ve
OLE-100 gruplarında WBC’ler, kontrol grubuna göre, anlamlı şekilde gerilerken,
OLE-25 grubunda değişim gözlenmedi. Diyabet+OLE-25 grubunun kolesterol
değerlerinde, kontrol grubuna göre, anlamlı bir düşüş gözlenirken,
diyabet+OLE-100 grubundaki artış istatiksel düzeyde önemliydi. Diyabet+OLE-50 grubundaki artış
anlamlı değildi. Diyabet+OLE-25, diyabet+OLE-50 ve diyabet+OLE-100
gruplarında, trigliserid anlamlı derecede düşmüştü (p<0.05). İnfüzyon
uygulanan diyabetli grupta RBC, HGB, HCT, MCV,
MPV, PDW ve EOS pozitif yönde anlamlı şekilde artmıştı (p<0.05). İnfüzyon
trigliserid, LDL, Cl ve Na’u negatif yönde, HDL, Mg ve P’u pozitif yönde
etkilediği belirlendi. OLE uygulanan gruplar içerisinde kolesterol ve
trigliserid üzerine en etkili dozun OLE-25 mg/kg olduğu gözlendi. İnfüzyonun
ise, HDL’yi pozitif yönde, LDL’yi de negatif yönde anlamlı şekilde etkilediği
görüldü. Aynı şekilde kolesteroldeki düşüşün önemsiz, trigliseritdeki düşüşün
ise önemli olduğu yönünde bulgular elde edildi.
Keywords
References
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- Andreadou, I., Iliodromitis, E.K., Mikros, E., Constantinou, M., Agalias, A., Magiatis, P., Skaltsounis, A.L., Kamber, E., Tsantili-Kakoulidou, A. and Kremastinos, D.T, 2006. The olive constituentol europein exhibits anti-ischemic, antioxidative, and hypolipidemic effects in anesthetized rabbits. Journal of Nutrition, 136, 2213-2219.
- Baynes, R.D., Lamparellı, R.D.V., Chetty, N., Atkınson, P., Bezwoda, W.R.A. ve Gear, J., 1988. Platelet parameters: Part II. Platelet volume-number relationships in various normal and disease states. SAMJ, 73, 39–43.
- Beyazit, Y., Sayilir, A., Torun, S., Suvak, B., Yeşil, Y. and Purnak, T., 2012. Mean platelet volume as an indicator of disease severity in patients with acute pancreatitis. Clin Res Hepatol Gastroenterol, 36, 162-8.
- Chandra, A., Mahdi, A.A., Ahmad, S. and Singh, R.K., 2007. Indian herbs result in hypoglycemic responses in streptozotocin induced diabetic rats. Nutr. Res, 27, 161-168.
- Choi, J.W., Pai, S.H., 2004. Influences of hypercholesterolemia on red cell indices and erythrocyte sedimentation rate in el-derly persons. Clin Chima Acta, 341, 117-121.
- Eidi, E., Eidi, M., Darzi, R., 2009. Antidiabetic effect of Olea europaea L. in normal and diabetic Rats. Phytotherapy Research, 23, 347-350.
- Gao, L.P., Li, Z., Guo, Z.Y., 2013. The effects of vitamin Con DDP-induced anemia in rats. Toxicol Mech Methods, 23, 383–388.
- Gayathrı, M., Kannabıran, K., 2009. The Effects of Oral Administration of an Aqueous Extract of Ficus bengalensis Stem Bark on Some Hematological and Biochemical Parameters in Rats with Streptozotocin-Induced Diabetes. Turk J Biol, 33, 9-13.
- Geyikoglu, F., Colak, S., Turkez, H., Bakır, M. and Koç, K., 2016. Oleuropein Ameliorates Cisplatin-induced HematologicalDamages Via Restraining Oxidative Stress and DNA Injury. Indian J Hematol Blood Transfus, DOI 10.1007/ s12288-016-0718-3
- Ginsberg, H.N., 1999. Identification and treatment of hypertri-glyceridemia as a risk factor for coronary heart disease. Curr Cardiol Rep, 1: 2337.
- Karthikesan, K., Pari, L. ve Menon, V.P., 2010. Combined treatment of tetrahydro curcumin and chlorogenic acid exert spotential antihyperglycemic effect on streptozotocin nicotinamide induced diabeticrats. Gen. Physiol. Biophys, 29, 23–30.
- Kayaalp, O., 2002. Rasyonel tedavi yönünden tıbbi farmakoloji. Onuncu baskı, Hacettepe-TAŞ, Ankara.
- Khayyal, M.T., El-Ghazaly, M.A., Abdallah, D.M., Nassar, N.N., Okpanyi, S.N. and Kreuter, M.H., 2002. Blood pressure lowering effect of an olive leaf extract (Olea europaea) in L-NAME induced hypertension in rats. Arzneimittelforschung, 52, 797–802.
- Klein, R., 1995. Hyperglycemia and microvascular and macrovascular disease in diabetes. Diabetes Care, 18, 258–268. Mahmoud, A.M., 2013. Hematological alterations in diabetic rats-role of adipocytokines and effect of citrus flavonoids. EXCLI Journal, 12, 647-57.
- Maimoona, A., Naeem, I., Saddiqe, Z. and Jameel, K., 2011. A review on biological, nutraceutical and clinical aspects of French maritimepine bark extract. J.Ethnopharmacol,133, 261-277.
- Meurs, I., Hoekstra, M., Van Wanrooij, E.J.A., Hildebrand, R.B, Kuiper, J., Kuipers, F., Hardeman, M.R., Van Berkel, T.J.C. and Van Eck, M., 2005. HDL cholesterol levels are an important factor for determining the lifespan of erythrocytes. Exp Hematol, 33, 1309-1319.
- Özbek, H., Cengiz, N., Him, A., Uğraş, S., Özgökçe, F. ve Erdoğan, E., 2006. Yüksek kolesterollü diyetle beslenen sıçanlarda Foeniculum vulgare P. mill. (rezene) tohumlarının kan kolesterol seviyesi üzerine etkis, Genel Tıp Derg,16, 177-180.
- Soler-Rivas, C., Espin, J.C. and Wichers, H.J., 2000. Oleuropein and related compounds. Journal of Agricultural and Food Chemistry, 80, 1013-1023.
- Somova, L.I., Shode, F.O., Mipando, M., 2004. Cardiotonic and antidysrhyth-mic effects of leanolic and ursolic acids, methyl maslinate and uvaol. Phytomedicine, 11, 121-129.
- Tancrède, G., Rousseau-Migneron, S. and Nadeau, A., 1983. Long-term changes in the diabetic state induced by different doses of streptozotocin in rats. British Journal of Experimental Pathology, 64, 117-123.
- Tedesco, I., Russo, M., Russo, P., Carraturo, A., Faruolo, C., Moio, L. and Palumbo, R., 2000. Antioxidant effect of red wine polyphenols on red blood cells. J Nutr Biochem, 11, 114–119.
- Thomas, R.M., 2002. Oxidative stress and lipid in diabetes. A role in endothelium vasodilator dysfunction. Vasc Med, 1, 195–204.
- Yılmaz, B., 2000. Fizyoloji. Canlılık olaylarıyla ilgili fiziksel ve kimyasal kurallar, beden sıvıları, kan, bağışıklık, alerji, lenf, kemik iliği ve kan dolaşımı, Ankara.
Year 2018,
Volume: 33 Issue: 1, 13 - 19, 30.06.2018
Abstract
References
- Al-Azzawie, H.F., Alhamdani, MS., 2006. Hypoglycemicand antioxidanteffect of oleuropein in alloxan-diabeticrabbits. Life Sci, 78, 1371-7.
- Andreadou, I., Iliodromitis, E.K., Mikros, E., Constantinou, M., Agalias, A., Magiatis, P., Skaltsounis, A.L., Kamber, E., Tsantili-Kakoulidou, A. and Kremastinos, D.T, 2006. The olive constituentol europein exhibits anti-ischemic, antioxidative, and hypolipidemic effects in anesthetized rabbits. Journal of Nutrition, 136, 2213-2219.
- Baynes, R.D., Lamparellı, R.D.V., Chetty, N., Atkınson, P., Bezwoda, W.R.A. ve Gear, J., 1988. Platelet parameters: Part II. Platelet volume-number relationships in various normal and disease states. SAMJ, 73, 39–43.
- Beyazit, Y., Sayilir, A., Torun, S., Suvak, B., Yeşil, Y. and Purnak, T., 2012. Mean platelet volume as an indicator of disease severity in patients with acute pancreatitis. Clin Res Hepatol Gastroenterol, 36, 162-8.
- Chandra, A., Mahdi, A.A., Ahmad, S. and Singh, R.K., 2007. Indian herbs result in hypoglycemic responses in streptozotocin induced diabetic rats. Nutr. Res, 27, 161-168.
- Choi, J.W., Pai, S.H., 2004. Influences of hypercholesterolemia on red cell indices and erythrocyte sedimentation rate in el-derly persons. Clin Chima Acta, 341, 117-121.
- Eidi, E., Eidi, M., Darzi, R., 2009. Antidiabetic effect of Olea europaea L. in normal and diabetic Rats. Phytotherapy Research, 23, 347-350.
- Gao, L.P., Li, Z., Guo, Z.Y., 2013. The effects of vitamin Con DDP-induced anemia in rats. Toxicol Mech Methods, 23, 383–388.
- Gayathrı, M., Kannabıran, K., 2009. The Effects of Oral Administration of an Aqueous Extract of Ficus bengalensis Stem Bark on Some Hematological and Biochemical Parameters in Rats with Streptozotocin-Induced Diabetes. Turk J Biol, 33, 9-13.
- Geyikoglu, F., Colak, S., Turkez, H., Bakır, M. and Koç, K., 2016. Oleuropein Ameliorates Cisplatin-induced HematologicalDamages Via Restraining Oxidative Stress and DNA Injury. Indian J Hematol Blood Transfus, DOI 10.1007/ s12288-016-0718-3
- Ginsberg, H.N., 1999. Identification and treatment of hypertri-glyceridemia as a risk factor for coronary heart disease. Curr Cardiol Rep, 1: 2337.
- Karthikesan, K., Pari, L. ve Menon, V.P., 2010. Combined treatment of tetrahydro curcumin and chlorogenic acid exert spotential antihyperglycemic effect on streptozotocin nicotinamide induced diabeticrats. Gen. Physiol. Biophys, 29, 23–30.
- Kayaalp, O., 2002. Rasyonel tedavi yönünden tıbbi farmakoloji. Onuncu baskı, Hacettepe-TAŞ, Ankara.
- Khayyal, M.T., El-Ghazaly, M.A., Abdallah, D.M., Nassar, N.N., Okpanyi, S.N. and Kreuter, M.H., 2002. Blood pressure lowering effect of an olive leaf extract (Olea europaea) in L-NAME induced hypertension in rats. Arzneimittelforschung, 52, 797–802.
- Klein, R., 1995. Hyperglycemia and microvascular and macrovascular disease in diabetes. Diabetes Care, 18, 258–268. Mahmoud, A.M., 2013. Hematological alterations in diabetic rats-role of adipocytokines and effect of citrus flavonoids. EXCLI Journal, 12, 647-57.
- Maimoona, A., Naeem, I., Saddiqe, Z. and Jameel, K., 2011. A review on biological, nutraceutical and clinical aspects of French maritimepine bark extract. J.Ethnopharmacol,133, 261-277.
- Meurs, I., Hoekstra, M., Van Wanrooij, E.J.A., Hildebrand, R.B, Kuiper, J., Kuipers, F., Hardeman, M.R., Van Berkel, T.J.C. and Van Eck, M., 2005. HDL cholesterol levels are an important factor for determining the lifespan of erythrocytes. Exp Hematol, 33, 1309-1319.
- Özbek, H., Cengiz, N., Him, A., Uğraş, S., Özgökçe, F. ve Erdoğan, E., 2006. Yüksek kolesterollü diyetle beslenen sıçanlarda Foeniculum vulgare P. mill. (rezene) tohumlarının kan kolesterol seviyesi üzerine etkis, Genel Tıp Derg,16, 177-180.
- Soler-Rivas, C., Espin, J.C. and Wichers, H.J., 2000. Oleuropein and related compounds. Journal of Agricultural and Food Chemistry, 80, 1013-1023.
- Somova, L.I., Shode, F.O., Mipando, M., 2004. Cardiotonic and antidysrhyth-mic effects of leanolic and ursolic acids, methyl maslinate and uvaol. Phytomedicine, 11, 121-129.
- Tancrède, G., Rousseau-Migneron, S. and Nadeau, A., 1983. Long-term changes in the diabetic state induced by different doses of streptozotocin in rats. British Journal of Experimental Pathology, 64, 117-123.
- Tedesco, I., Russo, M., Russo, P., Carraturo, A., Faruolo, C., Moio, L. and Palumbo, R., 2000. Antioxidant effect of red wine polyphenols on red blood cells. J Nutr Biochem, 11, 114–119.
- Thomas, R.M., 2002. Oxidative stress and lipid in diabetes. A role in endothelium vasodilator dysfunction. Vasc Med, 1, 195–204.
- Yılmaz, B., 2000. Fizyoloji. Canlılık olaylarıyla ilgili fiziksel ve kimyasal kurallar, beden sıvıları, kan, bağışıklık, alerji, lenf, kemik iliği ve kan dolaşımı, Ankara.
There are 24 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Publication Date | June 30, 2018 |
| Acceptance Date | January 1, 2018 |
| Published in Issue | Year 2018 Volume: 33 Issue: 1 |
