Year 2018, Volume 33 , Issue 1, Pages 43 - 49 2018-06-30

Antimicrobial Resistance Profiles and Tetracycline Resistance Genes of Escherichia coli in Mediterranean Mussel and Sea Snails Collected from Black Sea, Turkey

Ertuğrul Terzi [1]


Doğu Karadeniz (Türkiye)’den Toplanan Kara Midye ve Deniz Salyangozlarındaki Escherichia coli’lerin Antimikrobiyal Direnç Profilleri ve Tetrasiklin Direnç Genleri

Sucul ortamlar sık sık hastalıklardan korunma ve besin katkı maddesi olarak kullanılan antimikrobiyalleri de içeren karasal kökenli kirleticilere maruz kalmakta ve etkilenmektedir. Antimikrobiyal direnç dünya çapında hem insan sağlığında hem de hayvan sağlığı açısından büyük bir problemdir. Bu çalışmada, Karadeniz kıyılarından mevsimlik olarak kara midye ve deniz salyangozu örnekleri toplanmıştır. Artvin, Rize, Trabzon ve Giresun kıyılarından toplanan kara midye ve deniz salyangozlarında toplam 54 adet Escherichia coli suşu izole edilmiştir. E. coli izolatlarında antimikrobiyal direnç ve tetrasiklin direnç genlerinin (tetA, tetB, tetC, tetD, ve tetE) varlığı araştırılmıştır. Antimikrobiyal hassasiyet testi izolatların %83,3’ünün sulfametaksazola karşı direnç olduğunu göstermiştir. Ampisilin ve aztreonama karşı direnç de sırasıyla %66,7 ve %37,0 olarak hesaplanmıştır. En düşük direncin florfenikole karşı olduğu tespit edilmiştir. İzolatların % 50’sinden fazlasında tetC geni bulunmuştur. tet genleri arasında, tetC en çok bulunan gen olurken bunu tetB, tetA, tetE ve tetD takip etmiştir. İzolatların %88’inde en az bir tane test edilen direnç geni olduğu tespit edilmiş ve %46’sı ise iki veya daha fazla tet genine sahip olduğu belirlenmiştir. Sucul ortamlardaki E. coli’lerde tet direnç genlerinin varlığı bu izolatların tet direnç genleri bakımından bir rezervuar olabileceğini göstermektedir. Bu izolatlar ayrıca patojenik ve patojenik olmayan bakteriler arasında da bu genlerin yayılmasında önemli bir rol oynayabilir. 

Aquatic environments are often affected by and exposed to anthropogenic pollutants including antimicrobials used as disease prevention and feed additives. Antimicrobial resistance is a major problem both in animal and in human health worldwide. In this study, Mediterranean mussel (Mytilus galloprovincialis) and Sea snail (Rapana venosa) samples were collected seasonally from the coastline of Black Sea. A total of 54 Escherichia coli were isolated from Mediterranean mussel and Sea snail collected from the coast of Artvin, Rize, Trabzon and Giresun, Turkey. Antimicrobial resistance and the presence of tetracycline (tet) resistance genes (tetA, tetB, tetC, tetD, and tetE) in E. coli isolates were investigated. Antimicrobial susceptibility test determined that 83.3 % of the isolates exhibited resistance to sulfamethoxazole. Resistance to ampicillin and aztreonam was as 66.7 % and 37.0 % among the tested antimicrobials, respectively. The lowest resistant antimicrobial was florfenicol (1.9 %). tetC resistance gene was detected in more than 50% of the isolates. Among the tet resistance genes, tetC was found in the most common gene followed by tetB, tetA, tetE, and tetD. At least one tet gene was detected in 88% of the isolates, and 46% of the isolates had two or more tet genes. The presence of tet resistance genes in E. coli in aquatic environments indicates that these isolates may be a reservoir of tet resistance genes. They may also exhibit an important role in the spread of genes among the pathogenic and non-pathogenic bacteria.

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Primary Language en
Subjects Science
Published Date 2018-1
Journal Section Research Articles
Authors

Orcid: 0000-0003-2811-6497
Author: Ertuğrul Terzi
Institution: Kastamonu University
Country: Turkey


Dates

Publication Date : June 30, 2018

Bibtex @research article { alinterizbd355019, journal = {Alinteri Journal of Agriculture Science}, issn = {2564-7814}, eissn = {2587-2249}, address = {}, publisher = {Adem Yavuz SÖNMEZ}, year = {2018}, volume = {33}, pages = {43 - 49}, doi = {10.28955/alinterizbd.355019}, title = {Antimicrobial Resistance Profiles and Tetracycline Resistance Genes of Escherichia coli in Mediterranean Mussel and Sea Snails Collected from Black Sea, Turkey}, key = {cite}, author = {Terzi, Ertuğrul} }
APA Terzi, E . (2018). Antimicrobial Resistance Profiles and Tetracycline Resistance Genes of Escherichia coli in Mediterranean Mussel and Sea Snails Collected from Black Sea, Turkey. Alinteri Journal of Agriculture Science , 33 (1) , 43-49 . DOI: 10.28955/alinterizbd.355019
MLA Terzi, E . "Antimicrobial Resistance Profiles and Tetracycline Resistance Genes of Escherichia coli in Mediterranean Mussel and Sea Snails Collected from Black Sea, Turkey". Alinteri Journal of Agriculture Science 33 (2018 ): 43-49 <https://dergipark.org.tr/en/pub/alinterizbd/issue/38063/355019>
Chicago Terzi, E . "Antimicrobial Resistance Profiles and Tetracycline Resistance Genes of Escherichia coli in Mediterranean Mussel and Sea Snails Collected from Black Sea, Turkey". Alinteri Journal of Agriculture Science 33 (2018 ): 43-49
RIS TY - JOUR T1 - Antimicrobial Resistance Profiles and Tetracycline Resistance Genes of Escherichia coli in Mediterranean Mussel and Sea Snails Collected from Black Sea, Turkey AU - Ertuğrul Terzi Y1 - 2018 PY - 2018 N1 - doi: 10.28955/alinterizbd.355019 DO - 10.28955/alinterizbd.355019 T2 - Alinteri Journal of Agriculture Science JF - Journal JO - JOR SP - 43 EP - 49 VL - 33 IS - 1 SN - 2564-7814-2587-2249 M3 - doi: 10.28955/alinterizbd.355019 UR - https://doi.org/10.28955/alinterizbd.355019 Y2 - 2018 ER -
EndNote %0 Alınteri Zirai Bilimler Dergisi Antimicrobial Resistance Profiles and Tetracycline Resistance Genes of Escherichia coli in Mediterranean Mussel and Sea Snails Collected from Black Sea, Turkey %A Ertuğrul Terzi %T Antimicrobial Resistance Profiles and Tetracycline Resistance Genes of Escherichia coli in Mediterranean Mussel and Sea Snails Collected from Black Sea, Turkey %D 2018 %J Alinteri Journal of Agriculture Science %P 2564-7814-2587-2249 %V 33 %N 1 %R doi: 10.28955/alinterizbd.355019 %U 10.28955/alinterizbd.355019
ISNAD Terzi, Ertuğrul . "Antimicrobial Resistance Profiles and Tetracycline Resistance Genes of Escherichia coli in Mediterranean Mussel and Sea Snails Collected from Black Sea, Turkey". Alinteri Journal of Agriculture Science 33 / 1 (June 2018): 43-49 . https://doi.org/10.28955/alinterizbd.355019
AMA Terzi E . Antimicrobial Resistance Profiles and Tetracycline Resistance Genes of Escherichia coli in Mediterranean Mussel and Sea Snails Collected from Black Sea, Turkey. Alinteri Journal of Agriculture Science. 2018; 33(1): 43-49.
Vancouver Terzi E . Antimicrobial Resistance Profiles and Tetracycline Resistance Genes of Escherichia coli in Mediterranean Mussel and Sea Snails Collected from Black Sea, Turkey. Alinteri Journal of Agriculture Science. 2018; 33(1): 49-43.