Meme Kanseri Hücre Dizisi Üzerinde (MCF-7) Oğulotu (Melissa Officinalis) Bitki Ekstresinin Antiproliferatif, Apoptotik ve Antioksidan Etkilerinin Araştırılması

Year 2023, , 136 - 144, 31.01.2023

Serdal Öğüt , Ömer Erdoğan , Aslıhan Büyüköztürk Karul

https://doi.org/10.46237/amusbfd.1194803

Abstract

Amaç: Meme kanseri, dünyada ve ülkemizde kadınlarda en sık teşhis edilen kanser türlerinden biridir. Dünya geneline bakıldığında ölüm sebepleri arasında kadınlarda ikinci sırada yer aldığı bilinmektedir. Bu çalışmanın amacı, Melissa officinalis ekstresinin meme kanseri hücre dizisinde (MCF-7) antiproliferatif, apoptotik ve antioksidan etkilerinin belirlenmesidir.
Yöntem: Araştırmada oğulotu bitki ekstresinin farklı konsantrasyonları (1 µg/mL, 10 µg/mL, 100 µg/mL ve 1000 µg/mL) MCF-7 hücre dizisi üzerine uygulanmıştır. Sitotoksik aktiviteleri 24 saatte MTT yöntemi, apoptotik aktiviteleri ise muse anneksin V yöntemi kullanılarak belirlenmiştir. Biyokimyasal parametrelerin belirlenmesinde 1 µg/mL, 10 µg/mL, 100 µg/mL ve 1000 µg/mL dozlarda hücrelere uygulama yapılmış ve sonra hücre lizatları elde edilmiştir. Hazırlanan numuneler ile ilk olarak hücrenin protein düzeyi hesaplanmıştır. Ardından TOS, TAS, OSİ, SOD, GPx aktivite tayini, MDA ve NO analizleri yapılmıştır.
Bulgular: Sonuçlar değerlendirildiğinde, araştırmamızda doz ve zamana bağlı olarak Melissa officinalis ekstresinin MCF-7 hücre proliferasyonunu azalttığı belirlenmiştir. Aynı zamanda elde ettiğimiz apoptoz artışı da bu proliferasyon inhibisyonunu açıklamaktadır. Araştırma çerçevesinde ortaya çıkan antioksidan sonuçları da proliferasyonun inhibisyonunu ve aynı zamanda apoptozun artışını desteklemektedir.
Sonuç: İstatistiksel olarak tüm veriler yorumlandığında Melissa officinalis ekstresinin, MCF-7 hücrelerinde moleküler antikanserojenik mekanizmaları, tekli ya da kemoterapötik ajanlarla kombine çalışılarak, meme kanseri tedavisi için yeni kemoterapötik ve kemopreventif ajanların gelişimine önemli katkı sağlayacağı mevcut araştırma ile belirlenmiştir.

Keywords

Antioksidan parametreler, Apoptoz, MCF-7, Melissa offinalis

Supporting Institution

Adnan Menderes Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

TPF-19040

Investigation of Antiproliferative, Apoptotic and Antioxidant Effects of Lemon Balm (Melissa Officinalis) Plant Extract on Breast Cancer Cell Line (MCF-7)

Abstract

Objective: Breast cancer is one of the most frequently diagnosed cancer types in women in the world and in our country. It is known that it is the second leading cause of death in women worldwide. The aim of this study is to determine the antiproliferative, apoptotic and antioxidant effects of Melissa officinalis extract on breast cancer cell line (MCF-7).
Methods: In the study, different concentrations of Melissa officinalis extract (1 µg/mL, 10 µg/mL, 100 µg/mL and 1000 µg/mL) were applied to the MCF-7 cell line. Cytotoxic activities were determined using the MTT method at 24 hours, and apoptotic activities were determined using the muse annexin V method. To determine the biochemical parameters, 1 µg/mL, 10 µg/mL, 100 µg/mL and 1000 µg/mL doses were applied to the cells and then cell lysates were obtained. Firstly, the protein level of the cell was calculated with the prepared samples. Then, TOS, TAS, OSI, SOD, GPx activity determination, MDA and NO analyzes were investigated.
Results: When the results were evaluated, it was determined in our study that Melissa officinalis extract decreased MCF-7 cell proliferation depending on dose and time. At the same time, the increase in apoptosis we obtained explains this proliferation inhibition. The antioxidant results that emerged within the framework of the research also support the inhibition of proliferation and also the increase of apoptosis.
Conclusion: When all the statistical data are interpreted, it has been determined by the current research that the molecular anticarcinogenic mechanisms of Melissa officinalis extract in MCF-7 cells will contribute to the development of new chemotherapeutic and chemopreventive agents for breast cancer treatment by working alone or in combination with chemotherapeutic agents.

Keywords

Antioxidant parameters, Apoptosis, MCF-7, Melissa offinalis

Project Number

TPF-19040

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