Levels of Aromatase and Estrogen Receptors mRNA in Early Larvae of Alburnus tarichi (Güldenstӓdt, 1814) Exposed to Di-(2-ethylhexyl)-phthalate

Abstract

Objective: The aim of this study is to determine the effect of phthalate on the early larval development of Alburnus tarichi.
Methods: The larva (2 days after hatching) of Alburnus tarichi were exposed to 0.1, 1, and 10 µg/L phthalate for 6 days (during yolk sac nutrition), and the levels of estrogen receptor alfa, beta-1 and beta-2, and aromatase B and A mRNA levels were measured.
Results: No difference was found in the estrogen receptor alpha, beta-1, beta-2, and aromatase-B mRNA levels with all 3 concentrations of phthalate. However, the aromatase-A mRNA level was significantly increased with 0.1 µg/L of phthalate, while no changes were observed with 1 and 10 µg/L of phthalate.
Conclusion: These results suggest that short-time expose to environmentally relative concentrations of phthalate do not significantly affect genes in steroid synthesis during the early larval development of Alburnus tarichi. However, long-term applied studies including external nutrition are need to determination the effect of phthalate on steroid gene expression.

Keywords

• Alburnus tarichi,di-(2-ethylhexyl)-phthalate,Estrogen Receptor,P450 Aromatase

Project Number

2013-HIZ-FEN003

Di-(2-ethylhexyl)-phthalate’e Maruz Kalan Alburnus tarichi (Güldenstӓdt, 1814) Erken Larvalarında Östrojen Reseptör ve P450 Aromataz mRNA Seviyeleri

Abstract

Amaç: Bu çalışmanın amacı Alburnus tarichi’nin erken larval gelişimi üzerine fitalatın etkisini belirlemektir.
Yöntem: Erken larval dönemde (yumurtadan çıktıktan 2 gün sonra) 0.1, 1, ve 10 µg/L fitalat’a, 6 gün, maruz bırakılarak larvalarda östrojen reseptörleri alfa, beta-1 ve beta-2 ve aromataz B ve A’nın mRNA seviyeleri ölçüldü.
Bulgular: Fitalat’ın her üç konsantrasyonunda östrojen reseptör alfa, beta1, beta2 ve aromataz B’nin mRNA seviyelerinde belirgin bir değişiklik olmadığı belirlendi. Bununla birlikte aromataz A mRNA seviyesi fitalat’ın 0.1 µg/L uygulamasında belirgin olarak artarken 1 ve 10 µg/L uygulamalarda değişiklik gözlenmedi.
Sonuç: Bu sonuçlar, fitalat’ın çevresel konsantrasyonlarının kısa sureli uygulamalarda, Alburnus tarichi erken larval gelişimi sırasında, steroid sentezindeki genleri önemli seviyede etkilemediğini göstermiştir. Bununla birlikte fitalat’ın gen ekspresyonu üzerindeki etkisini belirlemek için, dış beslenmeyi de içine alan uzun süreli uygulamalı çalışmalara ihtiyaç vardır.  

Keywords

• Alburnus tarichi,Fitalat,Östrojen Reseptör,Gen Ekspresyonu,P450 Aromataz

Supporting Institution

Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı

Project Number

2013-HIZ-FEN003

Thanks

Yüzüncü Yıl Üniversitesi BAPB, Kellie A. Cotter ve Rebecca Meyer

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