Comparison of Two Different Serological Methods in The Diagnosis of Rotavirus and Adenovirus in Stool Samples

Year 2024, Volume: 8 Issue: 3, 262 - 269, 30.09.2024

Oktay İlhan , Mehmet Tevfik Bayraktar , Bashar Ibrahim

https://doi.org/10.46237/amusbfd.1275682

Abstract

Objective: Rotaviruses are the most common cause of acute infectious diarrhea in children under 5 years of age worldwide. This study aims to compare the immunochromatographic and ELISA methods, determine the sensitivity and specificity of the methods used, and shed light on humanity to diagnose the disease correctly.
Method: Stool samples of 1000 patients were sent to Diyarbakır Pediatric Hospital for rapid antigen testing of Rotavirus. Single-step Rotavirus and Adenovirus Co-immunochromatographic and ELISA methods were used in the examination of the samples. Samples with positive rapid test results were collected in 1.5 ml microcentrifuge tubes, and positive antigen samples collected for the ELISA test were stored at -80°C until the time of study.
Results: Of 1000 stool samples analyzed, 40 (11.59%) of 345 girls and 60 (9.16%) of 655 boys had Rotavirus antigen in a total of 100 (10%) and 20 (2%) had Adenovirus antigen (P> 0.05). Viral antigen-positive cases were most frequently seen in winter months (December, January, February). There was no statistically significant difference between the data in terms of age and gender (P <0.05).
Conclusion: In this study, Rotavirus was detected in one-tenth of diarrhea cases in pediatric patients and it was revealed that it increased seasonally in winter months.

Keywords

Rotavirus, Gastroenteritis, ELISA, Immunochromatographic, Pediatrics

Dışkı Örneklerinde Rotavirüs ve Adinovirüs Tanısında İki Farklı Serolojik Yöntemin Karşılaştırılması

Abstract

Amaç: Tüm dünyada beş yaş altındaki çocuklardaki akut enfeksiyöz ishalin en sık nedeni rotavirüslerdir. Bu çalışma immünkromatografik ve Enzyme-Linked ImmunoSorbent Assay (ELİSA) yöntemlerinin karşılaştırılması, kullanılan yöntemlerin duyarlılık ve özgüllüklerinin belirlenmesi ve hastalığın doğru teşhis edilebilmesi için insanlığa ışık tutmayı amaçlamaktadır.
Metod: Toplam 1000 hastanın Rotavirüs hızlı antijen testi çalışılması amacıyla Diyarbakır Çocuk Hastalıkları Hastanesi’ne gönderilen dışkı örnekleri çalışıldı. Örneklerin incelenmesinde tek aşamalı Rotavirüs ve Adenovirüs Birlikte immünokromatografik ve ELISA yöntemleri kullanıldı. Hızlı test sonucu pozitif olan örnekler 1.5 ml’lik mikrosantrifüj tüplerine alındı, ELISA testi için toplanan pozitif antijen numuneleri -80°C’de çalışılacağı zamana kadar saklandı. Bulgular: Analiz edilen 1000 dışkı örneğinin 345 kız çocuğunun 40' ında (%11.59) ve 655 erkek çocuğunun 60'ında (%9.16) toplam 100'ünde (%10) Rotavirüs antijeni ve 20’sinde (%2) Adenovirüs antijeni saptanmıştır (P>0,05). Viral antijen pozitif olgular en sık kış ayları (Aralık, Ocak, Şubat)’nda görüldü. Verilerin yaş ve cinsiyet yönünden istatistiksel olarak anlamlı bir farkı bulunmamıştır (P <0,05). Sonuç: Bu çalışmada çocuk hastalarındaki ishal vakalarının onda birinde Rotavirus tespit edilmiş ve kış aylarında da mevsimsel artış gösterdiği ortaya konmuştur.

Keywords

Rotavirüs, Gastroenterit, ELİSA, Immünokromatografik, Pediatri

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