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NaCl ve PEG’e Bağlı Osmotik Stresin Yabani Hardal (Sinapis arvensis L.)’ın Çimlenme ve Fide Gelişimi Özellikleri Üzerine Etkisi

Year 2018, Volume: 28 Issue: 1, 62 - 68, 15.05.2018

Abstract

ÖZ: Bu çalışma farklı NaCl ve PEG uygulamalarının yabani
hardalın (Sinapis
arvensis
L.) çimlenme ve erken vejetatif
gelişme üzerine etkisini belirlemek amacıyla 2016 yılında yürütüldü. Çalışmada
kullanılan yabani hardal tohumları Konya’da yabani koşullarda yetişen
bitkilerden alınmıştır. Denemede yedi farklı polietilen glikol
(PEG-6000) solüsyonu (0, -2, -4, -6, -8, -10 ve -12 bar) ve dokuz farklı NaCI
solüsyonu (0, 2, 5, 10, 15, 20, 25, 30 ve 35 dSm-1) kullanılmıştır.
Farklı PEG ve NaCl uygulamalarında kök uzunluğu (cm), sürgün uzunluğu (cm),
fide yaş ağırlığı (mg), fide kuru ağırlığı (mg), final çimlenme oranı (%),
çimlenme indeksi (%) ve fide canlılık indeksi ölçümleri yapılmıştır. Çalışma
sonucunda, final çimlenme oranının ve fide büyüme parametrelerinin artan PEG
konsantrasyonlarına (-4 / -10 bar) bağlı olarak azaldığı; final çimlenme
oranının 10 dSm-1 (-4,4 bar)’den itibaren azalmaya başladığı ve fide
büyüme parametrelerinin 5 dSm-1 (-2,2 bar)’den itibaren azalmaya
başladığı belirlenmiştir. Ayrıca, NaCl ve PEG uygulamalarının çimlenme ve fide
gelişimini düşürdüğü; fakat PEG’e kıyasla NaCl’ün etkisinin çimlenmede daha az
olduğu; çimlenmenin -12 bar PEG ve 35 dSm-1 (-15,6 bar) NaCl
dozlarında tamamen durduğu tespit edilmiştir.


References

  • Anonymous. 1996. International Rules for Seed Testing, Rules (ISTA) 1996. Seed Sci. Technol. 24 Suppl.
  • Ashraf, M., and T. McNeilly. 2004. Salinity tolerance in Brassica oilseeds. Crit. Rev. Plant Sci. 23: 157-174.
  • Asmare, H. A. 2013. Impact of salinity on tolerance, vigor, and seedling relative water content of haricot bean (Phaseolus vulgaris L.) cultivars. Journal of Plant Sci. 1 (3): 22-27.
  • Ayaz, F. A., A. Kadıoğlu, and R. T. Utgut. 2000. Water stress effects on the content of low molecular weight carbohydrates and phenolic acids in cienanthe serosa. Canadian J. Plant Sci. 80: 373-378.
  • Baranova, E. N., and A. A. Gulevich, and V. Y. Polyakov. 2006. Effects of NaCl, Na2SO4, and mannitol on storage lipid mobilization in the cotyledons and roots of purple alfalfa seedlings. Russian Journal of Plant Physiology 53: 779-784.
  • Bing, D. J., R. K. Downey, and G. F. W. Rakow. 1996. Assessment of transgene escape from Brassica rapa (B. Campestris L.) into Brassica nigra of Sinapis arvensis. Plant Breeding 115: 1-4.
  • Blackshaw, R., E. Johnson, Y. Gan, W. May, D. McAndrew, V. Barthet, T. McDonald, and D. Wispinski. 2011. Alternative oilseed crops for biodiesel feedstock on the Canadian prairies. Canadian Journal of Plant Sci. 9 (5): 889-896.
  • Böhm, W. 1979. Methods of Studying Root Systems, Springer-Verlag, Berlin.
  • Bybordi, A., and J. Tabatabaei. 2009. Effect of salinity stress on germination and seedling properties in Canola cultivars (Brassica napus L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca 37 (1): 71-76.
  • Eryilmaz, T., and H. Ogut. 2011. The effect of the different mustard oil biodiesel blending ratios on diesel engines performance. Energy Education Science and Technology Part A-Energy Science and Research 28(1):169-180.
  • Fallah-Toosi, A., and B. B. Baki. 2013. Effect of NaCl on Germination and Early Seedling Growth of Brassica juncea var. Ensabi. International Journal of Agronomy and Plant Production 4 (11): 3004-3011.
  • Francois, L. E. 1996. Salinity effects on four sunflower hybrids. Agronomy Journal 88 (2): 215-219.
  • Greenway, H., and R. Munns. 1980. Mechanisms of Salt Tolerance in Nonhalophytes. Annu. Rev. Plant Phy. 31: 149-190.
  • Gulzar, S., and M. A. Khan. 2001. Germination of a holophytic grass Aehtopus lagopoides. J. Ann. Bot. 87: 3119-3329.
  • Heshmat, O., H. A. Saeed, and K. Fardin. 2011. The improvememt of seed germination traits in canola (Brassica napus L.) as affected by saline and drought stress Journal of Agricultural Technology 7 (3): 611-622.
  • Huang, J., and R. E. Redmann. 1995. Salt Tolerance of Hordeum and Brassica Species During Germination and Early Seedling Growth. Can. J. Plant Sci. 75: 815-819.
  • Jamil, M., D. B., Lee, K. Y., Yung, M. Ashraf, S. C. Lee, and E. S. Rha. 2006. Effect of salt (NaCl) stress on germination and early seedling growth of four vegetables species. Journal of Central European Agriculture 7 (2): 273-281.
  • Kandil, A.A., A.E. Sharief, W.A.E. Abido, and M.M.O. Ibrahim. 2012. Response of some canola cultivars (Brassica napus L.) to salinity stress and its effect on germination and seedling properties. J. Crop Sci. 3:95-103.
  • Kayacetin, F., H. Ogut, H. Oguz, I. Subasi, and H. Deveci. 2016. Determination of the Effect of Row Spacing, and Fall and Spring Sowing on Composition of Fatty Acid and Biodiesel Fuel Characteristics of Mustard (Sinapis arvensis L.). Ciência e Técnica Vitivinícola Journal (ISSN: 0254-0223) 21 (11): 54-69.
  • Khajeh-Hosseini, M., A. A., Powell, and I. J. Bingham. 2003. The interaction between salinity stress and seed vigor during germination of soybean seeds. Seed Sci. Technol. 31: 715-725.
  • Khan, M. A., and S. Gulzar. 2003. Germination responses of Sporobolus ioclados: A saline desert grass. J. Arid Environ. 55: 453-464.
  • Luan, Z., M. Xiao, D. Zhou, H. Zhang, Y. Tian, Y. Wu, B. Guan, and Y. Song. 2014. Effects of Salinity, Temperature, and Polyethylene Glycol on the Seed Germination of Sunflower (Helianthus annuus L.). Hindawi Publishing Corporation Scientific World Journal, Article ID 170418. Vol: 2014, 9 pages. http://dx.doi.org/10.1155/2014/170418.
  • Macar, K. T. 2008. Effects of Water Deficit Induced by PEG and NaCl on Chickpea (Cicer arietinum L.) Cultivars and Lines at Early Seedling Stages. G. U. Journal of Science 22 (1): 5-14.
  • Mohammadkhani, N., and R. Heidari. 2008. Effects of Drought Stress on Soluble Proteins in two Maize Varities. Turk J. Biol. 32: 23-30. Murillo-Amador, B., R. Lopez-Aguilar, C. Kaya, J. Larrinaga-Mayoral, and H. A. Flores. 2002. Comparative effect of NaCl and PEG on germination emergence and seedling growth of cowpea. Journal of Agronomy and Crop Sciences 188: 235-47.
  • Odabas, M. S., K. E. Temizel, O. Caliskan, N. Senyer, G. Kayhan, and E. Ergun. 2014. Determination of reflectance values of hypericum’s leaves under stress conditions using adaptive network based fuzzy inference system. Neural Network World 24 (1): 79-87.
  • Okcu, G., M. D. Kaya, and M. Atak. 2005. Effects of Salt and Drought Stresses on Germination and Seedling Growth of Pea (Pisum sativum L.). Turk J Agric For. 29: 237-242.
  • Prakash, S., Y. Takahata, P. B. Kirti, and V. L. Chopra. 1999. Biology of Brassica Coenospecies. pp. 59-106. In: Gomez-Campo C., (ed.) Cytogenetics. Elsevier Science, Amsterdam.
  • Rollins, R. C. 1981. Weeds of the Cruciferae (Brassicaceae) in North America. J. Arnold. Arbor. 62: 517-540.
  • Saeedipour, S. 2009. Appraisal of Some Physiological Selection Criteria for Evaluation of Salt Tolerance in Canola (Brassica napus L.). International Journal of Applied Agricultural Research 4 (2): 179-192.
  • Singh, M. 2011. Varietal differences of salinity tolerance at germination stage in mustard genotypes, Prog. Agric. 11 (2): 468-471.
  • Srivastava, S., B. Fristensky, and N. N. V Kav. 2004. Constitutive expression pf Pr10 protein enhances the germination of Brassica napus under saline condition. Plant Cell Physiol. 45: 1320-1324.
  • Taherkhani, T., N. Rahmani, and A. Pazoki. 2013. The effect of Hydro-priming on Germination of Mustard Seeds under Draught Stress Conditions. Life Science Journal, Vol:10, 3pages. http://www.lifesciencesite. com/lsj/life1003s/058_16381life1003s_392_395.pdf.
  • Temizel, K. E., M. S. Odabas, N. Senyer, G. Kayhan, S. Bajwa, O. Caliskan, and E. Ergun. 2014. Comparision of some models for estimation of reflectance of hypericum leaves under stress conditions. Central European Journal of Biology 9 (12): 1226-1234.
  • Tilaki, G. A. D., B. Behtari, and B. Behtari. 2009. Effect of Salt and Water Stress on the Germination of Alfaalfa (Medicago Sativa L.) Seed 2: 158-164.
  • Toosi, A. F., B. B. Bakar, and M. Azizi. 2014. Effect of Drought Stress by Using PEG 6000 on Germination and Early Seedling Growth of Brassica juncea Var. Ensabi, Scientific Papers. Series A. Agronomi Vol: 52: 360-363.
  • Warwick, S. I., and L. D. Black. 1991. Molecular systematics of Brassica and allied genera (Subtribe Brassicinae Brassicae)-chloroplast genome and cytodeme congruence. Theor. Appl. Genet. 82: 81-92.
  • Werner, J. E., and R. R. Finkelstein. 1995. Abidopsismutants with reducedc response to NaCl and osmotic stress. Physiologia Plantarum 93: 659-666.

Effect of NaCl and PEG-Induced Osmotic Stress on Germination and Seedling Growth Properties in Wild Mustard (Sinapis arvensis L.)

Year 2018, Volume: 28 Issue: 1, 62 - 68, 15.05.2018

Abstract

ABSTRACT: This study was conducted to evaluate the effect of
NaCl and peg-induced osmotic stress on the germination
and early seedling growth of wild mustard (Sinapis
arvensis
L.) in 2016. The seeds of mustard used in this study were
obtained from plants growing under wild conditions in the Konya, Turkey.
Trials were carried out to study the effects of osmotic potential of
polyethylene glycol (PEG-6000) solutions at seven levels (0, -2, -4, -6, -8,
-10 and -12 bars) and salt concentrations at eight levels (0, 2, 5, 10, 15, 20,
25, 30 and 35 dSm-1) on seedling growth of wild mustard. Under PEG
and salt stress the radicula length (cm), plumula length (cm), seedling
fresh weight (mg), seedling dry weight (mg), final germination percentage (%),
germination index (%) and seedling vigor index (%) were measured in the study.
Final germination percentage and seedling growth parameters decreased by
concentration of -4 to -10 bar for PEG stress; final germination percentage
decreased by 10 dSm-1 (-4.4 bar) and seedling growth parameters
decreased by 5 dSm-1 (-2.2 bar) for NaCl stress. Both NaCl and PEG
treatments decreased final germination percentage and seedling growth
properties in wild mustard, but the effects of NaCl compared to PEG were less
on germination. Seed germination was completely inhibited at -12 bar for PEG
stress, 35 dSm-1 (-15.6 bar) for NaCl stress.


References

  • Anonymous. 1996. International Rules for Seed Testing, Rules (ISTA) 1996. Seed Sci. Technol. 24 Suppl.
  • Ashraf, M., and T. McNeilly. 2004. Salinity tolerance in Brassica oilseeds. Crit. Rev. Plant Sci. 23: 157-174.
  • Asmare, H. A. 2013. Impact of salinity on tolerance, vigor, and seedling relative water content of haricot bean (Phaseolus vulgaris L.) cultivars. Journal of Plant Sci. 1 (3): 22-27.
  • Ayaz, F. A., A. Kadıoğlu, and R. T. Utgut. 2000. Water stress effects on the content of low molecular weight carbohydrates and phenolic acids in cienanthe serosa. Canadian J. Plant Sci. 80: 373-378.
  • Baranova, E. N., and A. A. Gulevich, and V. Y. Polyakov. 2006. Effects of NaCl, Na2SO4, and mannitol on storage lipid mobilization in the cotyledons and roots of purple alfalfa seedlings. Russian Journal of Plant Physiology 53: 779-784.
  • Bing, D. J., R. K. Downey, and G. F. W. Rakow. 1996. Assessment of transgene escape from Brassica rapa (B. Campestris L.) into Brassica nigra of Sinapis arvensis. Plant Breeding 115: 1-4.
  • Blackshaw, R., E. Johnson, Y. Gan, W. May, D. McAndrew, V. Barthet, T. McDonald, and D. Wispinski. 2011. Alternative oilseed crops for biodiesel feedstock on the Canadian prairies. Canadian Journal of Plant Sci. 9 (5): 889-896.
  • Böhm, W. 1979. Methods of Studying Root Systems, Springer-Verlag, Berlin.
  • Bybordi, A., and J. Tabatabaei. 2009. Effect of salinity stress on germination and seedling properties in Canola cultivars (Brassica napus L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca 37 (1): 71-76.
  • Eryilmaz, T., and H. Ogut. 2011. The effect of the different mustard oil biodiesel blending ratios on diesel engines performance. Energy Education Science and Technology Part A-Energy Science and Research 28(1):169-180.
  • Fallah-Toosi, A., and B. B. Baki. 2013. Effect of NaCl on Germination and Early Seedling Growth of Brassica juncea var. Ensabi. International Journal of Agronomy and Plant Production 4 (11): 3004-3011.
  • Francois, L. E. 1996. Salinity effects on four sunflower hybrids. Agronomy Journal 88 (2): 215-219.
  • Greenway, H., and R. Munns. 1980. Mechanisms of Salt Tolerance in Nonhalophytes. Annu. Rev. Plant Phy. 31: 149-190.
  • Gulzar, S., and M. A. Khan. 2001. Germination of a holophytic grass Aehtopus lagopoides. J. Ann. Bot. 87: 3119-3329.
  • Heshmat, O., H. A. Saeed, and K. Fardin. 2011. The improvememt of seed germination traits in canola (Brassica napus L.) as affected by saline and drought stress Journal of Agricultural Technology 7 (3): 611-622.
  • Huang, J., and R. E. Redmann. 1995. Salt Tolerance of Hordeum and Brassica Species During Germination and Early Seedling Growth. Can. J. Plant Sci. 75: 815-819.
  • Jamil, M., D. B., Lee, K. Y., Yung, M. Ashraf, S. C. Lee, and E. S. Rha. 2006. Effect of salt (NaCl) stress on germination and early seedling growth of four vegetables species. Journal of Central European Agriculture 7 (2): 273-281.
  • Kandil, A.A., A.E. Sharief, W.A.E. Abido, and M.M.O. Ibrahim. 2012. Response of some canola cultivars (Brassica napus L.) to salinity stress and its effect on germination and seedling properties. J. Crop Sci. 3:95-103.
  • Kayacetin, F., H. Ogut, H. Oguz, I. Subasi, and H. Deveci. 2016. Determination of the Effect of Row Spacing, and Fall and Spring Sowing on Composition of Fatty Acid and Biodiesel Fuel Characteristics of Mustard (Sinapis arvensis L.). Ciência e Técnica Vitivinícola Journal (ISSN: 0254-0223) 21 (11): 54-69.
  • Khajeh-Hosseini, M., A. A., Powell, and I. J. Bingham. 2003. The interaction between salinity stress and seed vigor during germination of soybean seeds. Seed Sci. Technol. 31: 715-725.
  • Khan, M. A., and S. Gulzar. 2003. Germination responses of Sporobolus ioclados: A saline desert grass. J. Arid Environ. 55: 453-464.
  • Luan, Z., M. Xiao, D. Zhou, H. Zhang, Y. Tian, Y. Wu, B. Guan, and Y. Song. 2014. Effects of Salinity, Temperature, and Polyethylene Glycol on the Seed Germination of Sunflower (Helianthus annuus L.). Hindawi Publishing Corporation Scientific World Journal, Article ID 170418. Vol: 2014, 9 pages. http://dx.doi.org/10.1155/2014/170418.
  • Macar, K. T. 2008. Effects of Water Deficit Induced by PEG and NaCl on Chickpea (Cicer arietinum L.) Cultivars and Lines at Early Seedling Stages. G. U. Journal of Science 22 (1): 5-14.
  • Mohammadkhani, N., and R. Heidari. 2008. Effects of Drought Stress on Soluble Proteins in two Maize Varities. Turk J. Biol. 32: 23-30. Murillo-Amador, B., R. Lopez-Aguilar, C. Kaya, J. Larrinaga-Mayoral, and H. A. Flores. 2002. Comparative effect of NaCl and PEG on germination emergence and seedling growth of cowpea. Journal of Agronomy and Crop Sciences 188: 235-47.
  • Odabas, M. S., K. E. Temizel, O. Caliskan, N. Senyer, G. Kayhan, and E. Ergun. 2014. Determination of reflectance values of hypericum’s leaves under stress conditions using adaptive network based fuzzy inference system. Neural Network World 24 (1): 79-87.
  • Okcu, G., M. D. Kaya, and M. Atak. 2005. Effects of Salt and Drought Stresses on Germination and Seedling Growth of Pea (Pisum sativum L.). Turk J Agric For. 29: 237-242.
  • Prakash, S., Y. Takahata, P. B. Kirti, and V. L. Chopra. 1999. Biology of Brassica Coenospecies. pp. 59-106. In: Gomez-Campo C., (ed.) Cytogenetics. Elsevier Science, Amsterdam.
  • Rollins, R. C. 1981. Weeds of the Cruciferae (Brassicaceae) in North America. J. Arnold. Arbor. 62: 517-540.
  • Saeedipour, S. 2009. Appraisal of Some Physiological Selection Criteria for Evaluation of Salt Tolerance in Canola (Brassica napus L.). International Journal of Applied Agricultural Research 4 (2): 179-192.
  • Singh, M. 2011. Varietal differences of salinity tolerance at germination stage in mustard genotypes, Prog. Agric. 11 (2): 468-471.
  • Srivastava, S., B. Fristensky, and N. N. V Kav. 2004. Constitutive expression pf Pr10 protein enhances the germination of Brassica napus under saline condition. Plant Cell Physiol. 45: 1320-1324.
  • Taherkhani, T., N. Rahmani, and A. Pazoki. 2013. The effect of Hydro-priming on Germination of Mustard Seeds under Draught Stress Conditions. Life Science Journal, Vol:10, 3pages. http://www.lifesciencesite. com/lsj/life1003s/058_16381life1003s_392_395.pdf.
  • Temizel, K. E., M. S. Odabas, N. Senyer, G. Kayhan, S. Bajwa, O. Caliskan, and E. Ergun. 2014. Comparision of some models for estimation of reflectance of hypericum leaves under stress conditions. Central European Journal of Biology 9 (12): 1226-1234.
  • Tilaki, G. A. D., B. Behtari, and B. Behtari. 2009. Effect of Salt and Water Stress on the Germination of Alfaalfa (Medicago Sativa L.) Seed 2: 158-164.
  • Toosi, A. F., B. B. Bakar, and M. Azizi. 2014. Effect of Drought Stress by Using PEG 6000 on Germination and Early Seedling Growth of Brassica juncea Var. Ensabi, Scientific Papers. Series A. Agronomi Vol: 52: 360-363.
  • Warwick, S. I., and L. D. Black. 1991. Molecular systematics of Brassica and allied genera (Subtribe Brassicinae Brassicae)-chloroplast genome and cytodeme congruence. Theor. Appl. Genet. 82: 81-92.
  • Werner, J. E., and R. R. Finkelstein. 1995. Abidopsismutants with reducedc response to NaCl and osmotic stress. Physiologia Plantarum 93: 659-666.
There are 37 citations in total.

Details

Primary Language English
Journal Section Makaleler
Authors

Fatma Kayaçetin

Banu Efeoğlu This is me

Behrouz Alızadeh This is me

Publication Date May 15, 2018
Submission Date July 7, 2017
Published in Issue Year 2018 Volume: 28 Issue: 1

Cite

APA Kayaçetin, F., Efeoğlu, B., & Alızadeh, B. (2018). Effect of NaCl and PEG-Induced Osmotic Stress on Germination and Seedling Growth Properties in Wild Mustard (Sinapis arvensis L.). ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi, 28(1), 62-68.
AMA Kayaçetin F, Efeoğlu B, Alızadeh B. Effect of NaCl and PEG-Induced Osmotic Stress on Germination and Seedling Growth Properties in Wild Mustard (Sinapis arvensis L.). ANADOLU. May 2018;28(1):62-68.
Chicago Kayaçetin, Fatma, Banu Efeoğlu, and Behrouz Alızadeh. “Effect of NaCl and PEG-Induced Osmotic Stress on Germination and Seedling Growth Properties in Wild Mustard (Sinapis Arvensis L.)”. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi 28, no. 1 (May 2018): 62-68.
EndNote Kayaçetin F, Efeoğlu B, Alızadeh B (May 1, 2018) Effect of NaCl and PEG-Induced Osmotic Stress on Germination and Seedling Growth Properties in Wild Mustard (Sinapis arvensis L.). ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi 28 1 62–68.
IEEE F. Kayaçetin, B. Efeoğlu, and B. Alızadeh, “Effect of NaCl and PEG-Induced Osmotic Stress on Germination and Seedling Growth Properties in Wild Mustard (Sinapis arvensis L.)”, ANADOLU, vol. 28, no. 1, pp. 62–68, 2018.
ISNAD Kayaçetin, Fatma et al. “Effect of NaCl and PEG-Induced Osmotic Stress on Germination and Seedling Growth Properties in Wild Mustard (Sinapis Arvensis L.)”. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi 28/1 (May 2018), 62-68.
JAMA Kayaçetin F, Efeoğlu B, Alızadeh B. Effect of NaCl and PEG-Induced Osmotic Stress on Germination and Seedling Growth Properties in Wild Mustard (Sinapis arvensis L.). ANADOLU. 2018;28:62–68.
MLA Kayaçetin, Fatma et al. “Effect of NaCl and PEG-Induced Osmotic Stress on Germination and Seedling Growth Properties in Wild Mustard (Sinapis Arvensis L.)”. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi, vol. 28, no. 1, 2018, pp. 62-68.
Vancouver Kayaçetin F, Efeoğlu B, Alızadeh B. Effect of NaCl and PEG-Induced Osmotic Stress on Germination and Seedling Growth Properties in Wild Mustard (Sinapis arvensis L.). ANADOLU. 2018;28(1):62-8.
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