Abstract
In this study Cytotoxic effects of radioiodine (I131) which is a radionuclide used for the diagnosis and treatment of thyroid cancers and hyperthyroidism on mitotic chromosomes of root tips meristematic cells of fava bean [Vicia faba L. (Fabaceae)] were investigated. Seeds treated with radioiodine (I131) for 24, 48, 96 hours and 16 days were germinated. The root tips were taken and prepared for microscopic studies. Radioiodine (I131) caused chromosomal abnormalities as chromosome adherence, chromosome breaks, ring chromosome, chromatin bridge in V. faba L. root tip cells. The most common type of observed abnormalities was fish bone chromosome adherence, ring and C chromosome and chromosome dispersion. As a result of this study, it was determined that radioiodine (I131) had a mitodepressive effect on mitosis and harmful effects on V. faba L. root tip cells. The data obtained were evaluated numerically and statistical analysis was performed.
References
- Arambasic, M. B., S. Bjelic, and G. V. S. Simic. 1995. Acute toxicity of heavy metals (Copper, Lead, Zinc), phenol and sodium on Allium cepa L., Lepidium sativum L., Daphnia magna St. comparative ınvestigations and the practical applications. Water Research 29 (2): 497-503.
- Darlington, C. D., and L. F. La Cour. 1976. The Handling of Chromosomes. Allen and Unwin, London.
- Haugen, B. R., E. K. Alexander, K. C. Bible, G. M. Doherty, S. J. Mandel, Y. E. Nikiforov, F. Pacini, G. W. Randolph, A. M. Sawka, M. Schlumberger, K. G. Schuff, S. I. Sherman, J. A. Sosa, D. L. Steward, R. M. Tuttle, and L. Wartofsky. 2016. American Thyroid Association management guidelines for adult patients with thyroid nodules and differentiated thyroid cancer: The American Thyroid Association Guidelines Task Force on Thyroid Nodules and Differentiated Thyroid Cancer. Thyroid 26 (1): 1-133.
- İnceer, H., S. Ayaz, O. Beyazoğlu, and E. Şentürk. 2003. Cytogenetic effects of copper chloride on the root tip cells of Helianthus annuus L. Turkish Journal of Biology 27 (1): 43-46.
- Kasianenko, A. A., and G. A. Kulieva. 2002. Ecological consequences of depleted uranium use in ammunition. Bulletin of Russian Peoples’ Friendship University. Series Ecology and Life Safety-Moscow, Publishing House of RPFU 6: 90-99.
- Kıran, Y., and A. Sahin. 2005. The effects of the lead on the seed germination, root growth and root tip cell mitotic divisons of Lens culinaris Medik. Gazi Univ. Journal of Science 18 (1): 17-25.
- Leonard, A, P. Jacqued, and R. Lauverys. 1983. Mutagenicity and teratogenicity of mercury compounds. Mutat Res. 114 (1): 1-8. Özdemir, C., F. S. Erees, and S. Çam. 2008. Cytogenetic effects of uranium on root tip cells of Vicia faba L. Botanica Lithuanica 14 (3): 155-158.
- Parween, T., S. Jan, Mahmooduzzafar, M. P. Sharma, A. Mujib, and T. Fatma. 2011. Genotoxic impact of cadmium on root meristem of Vicia faba L. Russian Agricultural Sciences 37 (2): 115-119. https://doi.org/ 10.3103/S1068367411020248.
- Rivkees, S. A., C. Sklar, and M. Freemark. 1998. The Management of graves' disease in children, with special emphasis on radioiodine treatment. Journal of Clinical Endocrinology and Metabolism. 83 (11): 3767-3776.
- Simon, S., A. Bouville, and C. Land. 2006. Fallout from nuclear weapons tests and cancer risks. American Scientist 94 (1): 48-57. https://doi.org/10.1511/2006. 57.48.
- Rao, S. M. 2006. Radioisotopes of hydrological interest. In: Practical isotope hydrology. New Delhi: New India Publishing Agency 12-13.
- Valery, M. D. 2006. Biogenic iodine and iodine-containing metabolites. NPC Natural Product Communications 1 (2): 139-175.
- Yi, S., R. L. Wang, T. Feng, Y-C. Xiang, and H. Zhou. 2007. Effects of uranium tailings lixivium on early growth of Vicia faba. Journal of Hunan University of Science and Technology (Natural Science Edition) 4: 113-116.
Effects of Radioiodine (I131) on Mitotic Chromosomes of Root Meristem Cells of Vicia faba L. and Numerical Evaluation
Abstract
Bu çalışmada tiroid kanserlerinin ve hipertiroidizmin tanı ve tedavisinde kullanılan bir radyonüklid olan radyoiodinin (I131), baklanın [Vicia faba L. (Fabaceae)] kök uçlarındaki meristematik hücrelerinin mitotik kromozomları üzerindeki sitotoksik etkileri incelendi. Tohumlar radyoiodin ile 24, 48, 96 saat ve 16 gün sürelerle muamele edildi ve çimlendirildi. Çimlenen kök uçları alındı ve mikroskobik çalışmalar için hazırlandı. Radyoiodine (I131) V. faba L. kök ucu hücrelerinde kromozom yapışması, kromozom kopması, halka kromozom yapışması, köprü kromozom gibi kromozom anormalliklerine neden oldu. En çok görülen anormallikler balık kılçığı kromozom yapışması, halka ve C kromozom, kromozom dağılmasıdır. Bu çalışmanın sonucu olarak, radyoiyodinin mitoz üzerinde mitodepresif bir etkisi olduğu ve V. faba L. kök ucu hücreleri üzerinde zararlı etkileri olduğu tespit edildi. Elde edilen veriler sayısal olarak değerlendirilip istatistiksel analizleri yapıldı.
References
- Arambasic, M. B., S. Bjelic, and G. V. S. Simic. 1995. Acute toxicity of heavy metals (Copper, Lead, Zinc), phenol and sodium on Allium cepa L., Lepidium sativum L., Daphnia magna St. comparative ınvestigations and the practical applications. Water Research 29 (2): 497-503.
- Darlington, C. D., and L. F. La Cour. 1976. The Handling of Chromosomes. Allen and Unwin, London.
- Haugen, B. R., E. K. Alexander, K. C. Bible, G. M. Doherty, S. J. Mandel, Y. E. Nikiforov, F. Pacini, G. W. Randolph, A. M. Sawka, M. Schlumberger, K. G. Schuff, S. I. Sherman, J. A. Sosa, D. L. Steward, R. M. Tuttle, and L. Wartofsky. 2016. American Thyroid Association management guidelines for adult patients with thyroid nodules and differentiated thyroid cancer: The American Thyroid Association Guidelines Task Force on Thyroid Nodules and Differentiated Thyroid Cancer. Thyroid 26 (1): 1-133.
- İnceer, H., S. Ayaz, O. Beyazoğlu, and E. Şentürk. 2003. Cytogenetic effects of copper chloride on the root tip cells of Helianthus annuus L. Turkish Journal of Biology 27 (1): 43-46.
- Kasianenko, A. A., and G. A. Kulieva. 2002. Ecological consequences of depleted uranium use in ammunition. Bulletin of Russian Peoples’ Friendship University. Series Ecology and Life Safety-Moscow, Publishing House of RPFU 6: 90-99.
- Kıran, Y., and A. Sahin. 2005. The effects of the lead on the seed germination, root growth and root tip cell mitotic divisons of Lens culinaris Medik. Gazi Univ. Journal of Science 18 (1): 17-25.
- Leonard, A, P. Jacqued, and R. Lauverys. 1983. Mutagenicity and teratogenicity of mercury compounds. Mutat Res. 114 (1): 1-8. Özdemir, C., F. S. Erees, and S. Çam. 2008. Cytogenetic effects of uranium on root tip cells of Vicia faba L. Botanica Lithuanica 14 (3): 155-158.
- Parween, T., S. Jan, Mahmooduzzafar, M. P. Sharma, A. Mujib, and T. Fatma. 2011. Genotoxic impact of cadmium on root meristem of Vicia faba L. Russian Agricultural Sciences 37 (2): 115-119. https://doi.org/ 10.3103/S1068367411020248.
- Rivkees, S. A., C. Sklar, and M. Freemark. 1998. The Management of graves' disease in children, with special emphasis on radioiodine treatment. Journal of Clinical Endocrinology and Metabolism. 83 (11): 3767-3776.
- Simon, S., A. Bouville, and C. Land. 2006. Fallout from nuclear weapons tests and cancer risks. American Scientist 94 (1): 48-57. https://doi.org/10.1511/2006. 57.48.
- Rao, S. M. 2006. Radioisotopes of hydrological interest. In: Practical isotope hydrology. New Delhi: New India Publishing Agency 12-13.
- Valery, M. D. 2006. Biogenic iodine and iodine-containing metabolites. NPC Natural Product Communications 1 (2): 139-175.
- Yi, S., R. L. Wang, T. Feng, Y-C. Xiang, and H. Zhou. 2007. Effects of uranium tailings lixivium on early growth of Vicia faba. Journal of Hunan University of Science and Technology (Natural Science Edition) 4: 113-116.
Details
| Primary Language | English |
|---|---|
| Subjects | Agricultural, Veterinary and Food Sciences |
| Journal Section | Makaleler |
| Authors | |
| Publication Date | May 8, 2020 |
| Submission Date | December 12, 1902 |
| Published in Issue | Year 2020 Volume: 30 Issue: 1 |
