Investigation of DNA Methylation Level in Wheat Genome Exposed to Vanadium by Using CRED-RA Technique

Year 2021, Volume: 31 Issue: 1, 36 - 46, 15.06.2021

Özlem Bakır , Güleray Ağar

https://doi.org/10.18615/anadolu.949925

Cited By: 1

Abstract

Vanadium (V) has become a serious pollutant due to its widespread use in industry. In this study, DNA methylation changes in Triticum aestivum L. 'Çetinel 2000' seeds were determined by CRED-RA (Coupled Restriction Enzyme Digestion-Random Amplification) analysis. DNA damage and genomic instability were determined by Simple Repeat Inter-Sequence Polymorphism (ISSR) analysis. Vanadium solutions (4.4 mM, 6.6 mM, 8.8 mM) and boric acid (BA) solutions (4 mM and 8 mM) were used. According to CRED-RA analysis, it was observed that V caused more changes in the DNA methylation level in wheat genome compared to BA application. All doses of V caused DNA damage and decreased genomic template stability (GTS). It was determined that the genomic stability was maintained in BA doses applied with V. Genomic stability was maintained at BA doses administered with V. As a result, BA may be an alternative to reduce genotoxic damage to V stress in plants.

Keywords

Boric acid, wheat, CRED-RA, DNA methylation, Triticum aestivum L., vanadium

Vanadyuma Maruz Kalan Buğday Genomunda DNA Metilasyon Düzeyinin CRED-RA Tekniği Kullanılarak Araştırılması

Abstract

Vanadyum (V), endüstride yaygın kullanımı nedeniyle ciddi bir kirletici haline gelmiştir. Bu çalışmada, Triticum
aestivum L. ‘Çetinel 2000’ tohumlarındaki DNA metilasyon değişiklikleri CRED-RA (Çift Restriksiyon Enzimi Kesimi ve
Rastgele Çoğaltım) analizleri ile belirlenmiştir. DNA hasarları ve genomik kararsızlık Basit Tekrarlı Diziler Arası Polimorfizm (ISSR) analizi ile belirlenmiştir. Vanadyum çözeltileri (4,4 mM, 6,6 mM, 8,8 mM) ve borik asit (BA) çözeltileri (4 mM ve 8 mM) kullanılmıştır. CRED-RA analizine göre, V uygulamasının buğday genomunda DNA metilasyon seviyesinde BA uygulamasına göre daha fazla değişikliğe neden olduğu görülmüştür. Tüm V dozları DNA hasarına sebep olarak genomik kararlılığın (GTS) azalmasına neden olmuştur. V ile birlikte uygulanan BA dozlarında genomik kararlılık korunmuştur. Sonuç olarak, BA'nın V stres koşulları altında bitkilerde genetik hasarı azaltmak için alternatif bir kimyasal olabileceği düşünülmektedir.

Keywords

Borik asit, buğday, CRED-RA, DNA metilasyonu, Triticum aestivum L., vanadyum

References

• Aihemaiti, A., Y. Gao, Y. Meng, X. Chen, J. Liu, H. Xiang, and J. Jiang. 2020. Review of plant-vanadium physiological interactions, bioaccumulation, and bioremediation of vanadium-contaminated sites. Science of The Total Environment 712:135637.
• Arslan, E. 2020. putrescine in herbicide stress protection: modulate the genomic ınstability and DNA methylation changes in wheat. Avrupa Bilim ve Teknoloji Dergisi (19): 442-448.
• Bakir, O., G. Agar. 2020. Ameliorating effect of boric acid against vanadium toxicity in wheat (Triticum aestivum L.). Arabian Journal for Science and Engineering. https://doi.org/10.1007/s13369-019-04109-w.
• Bal, W., A. M. Protas, and K. S. Kasprzak. 2011. Genotoxicity of metal ions: chemical insights. Metal Ions Life Science 8:319–373.
• Brown, P. H., N. Bellaloui, M.A. Wimmer, E.S. Bassil, J. Ruiz, H. Hu, V. Römheld. 2002. Boron in plant biology. Plant biology 4(2): 205-223.
• Cai, Q., L. C. Guy, and G. A. Moore. 1996. Detection of cytosine methylation and mapping of a gene influencing cytosine methylation in the genome of Citrus. Genome 39: 235-242.
• Cansaran, D., O. Atakol, and S. Aras. 2011. Assessment of air pollution genotoxicity by RAPD in Evernia prunastri L. Ach. from around iron-steel factory in Karabük, Turkey. Journal of Environmental Sciences 23 (7): 1171-117.
• Crans, D., S. Amin, and A. Keramidas. 1998. Chemistry of relevance to vanadium in the environment. Chemistry and Biochemistry 30:73–96.
• Cui, C., Y. Li, Y. Liu, X. Li, S. Luo, Z. Zhang, and P. Tian. 2017. Determination of genetic diversity among Saccharina germplasm using ISSR and RAPD markers. Comptes Rendus Biologies 340 (2):76-86.
• Çatav, Ş.S., T. O. Genç, M. K. Oktay, and K. Küçükakyüz. 2018. Efect of boron toxicity on oxidative stress and genotoxicity in wheat (Triticum aestivum L.). Bull. Environ. Contam. Toxicol. 100 (4): 502-508.
• Ellen, T.P., T. Kluz, M. E. Harder, J. Xiong, and M. Costa. 2009. Heterochromatinization as a potential mechanism of nickel-induced carcinogenesis. Biochemistry 48: 4626-4632.
• Evangelou, A. M. 2002. Vanadium in cancer treatment. Critical Reviews in Oncology/Hematology 42(3):249-265.
• Ge, X.C., H. Zong, S. Zhan, J. C. Chen, C. R. Sun, and K. M. Cao. 2002. Cloning and expression analysis of a human putative tumor suppressor gene homologue from rice. Acta Botanica Sinica 44 (5): 562-566.
• Gill, S.S., and N. Tuteja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol and Bioch 48 (12): 909-930.
• Imtiaz, M., M. S. Rizwan, S. Xiong, H. Li, M. Ashraf, S. M. Shahzad, and S. Tu. 2015. Vanadium, recent advancements and research prospects: a review. Environment International 80: 79-88.
• Kar, B.B., P. Datta, and V. N. Misra. 2004. Spent catalyst: secondary source for molybdenum recovery. Hydrometallurgy 72 (1-2): 87-92.
• Kekec, G., M. S. Sakcali, and I. Uzonur. 2010. Assessment of genotoxic effects of boron on wheat (Triticum aestivum L.) and bean (Phaseolus vılgaris L.) by using RAPD anallysis. Bull. Environ. Contam. Toxicol 84(6):759- 764.
• Leljak-Levanić, D., N. Bauer, S. Mihaljević, and S. Jelaska. 2004. Changes in DNA methylation during somatic embryogenesis in Cucurbita pepo L. Plant Cell Reports 23 (3):120-127.
• Li, G., and C. F. Quiros. 2001. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics 103(2-3):455- 461.
• Li, X., X. Yu, N. Wang, Q. Feng, Z. Dong, L. Liu, and B. Liu. 2007. Genetic and epigenetic instabilities induced by tissue culture in wild barley (Hordeum brevisubulatum (Trin.) Link). Plant Cell, Tissue and Organ Culture 90 (2): 153-168.
• Nawaz, M.A., Y. Jiao, C. Chen, F. Shireen, Z. Zheng, M. Imtiaz, and Y. Huang. 2018. Melatonin pretreatment improves vanadium stress tolerance of watermelon seedlings by reducing vanadium concentration in the leaves and regulating melatonin biosynthesis and antioxidant-related gene expression. J. Plant Physiology 220: 115–127.
• Reddy, M. P., N. Sarla, and E.A. Siddiq. 2002. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128 (1): 9-17.
• Rerkasem, B., and S. Jamyod. 1997. Genotypic Variation In Plant Respance to Low Boron and Implications For Plant Breeding. Kluwer Acamedic Publisters. Printed Nederland.
• Sharma, S. S., and K. J. Dietz. 2009. The relationship between metal toxicity and cellular redox imbalance. Trends in Plant Science 14(1):43-50.
• Sorrentino, M.C., F. Capozzi, S. Giordano, and V. Spagnuolo. 2017. Genotoxic effect of Pb and Cd on in vitro cultures of Sphagnum palustre: An evaluation by ISSR markers. Chemosphere 181: 208-215.
• Stankiewicz, P.J., A.S. Tracey, and D. C. Crans. 1995. Inhibition of phosphatemetabolizing enzymes by oxovanadium (V) complexes. Metal Ions in Biological Systems 31:287-287.
• Taspinar, M.S., M. Aydin, B. Sigmaz, S. Yagci, E. Arslan, and G. Agar. 2018. Aluminum-induced changes on DNA damage, DNA methylation and LTR retrotransposon polymorphism in maize. Arabian Journal for Science and Engineering 43 (1): 123-131.
• Turkez, H., F. Geyikoglu, A. Tatar, M. S. Keles, and İ. Kaplan. 2012. The effects of some boron compounds against heavy metal toxicity in human blood. Experimental and Toxicologic Pathology 64(1-2):93-101.
• Yang, L.T., Y. B. Lu, Y. Zhang, P. Guo, and L. S. Chen. 2016. Proteomic profile of Citrus grandis roots under long-term boron-deficiency revealed by iTRAQ. Trees 30 (4):1057-1071.
• Wu, G., H. Zhou, P. Zhang, X. Wang, W. Li, W. Zhang, X. Liu, H. W. Liu, N. P. Keller, Z. An, and W. B. Yin. 2016. Polyketide production of pestaloficiols and macrodiolide ficiolides revealed by manipulations of epigenetic regulators in an endophytic fungus. Org Lett. 18:1832–1835.