Entomopatojen nematod Heterorhabditis bacteriophora'nın (Rhabditida: Heterorhabditidae) kontrollü koşullar altında patates güvesi (Phthorimaea operculella (Zeller)) (Lepidoptera: Gelechiidae) üzerinde etkinliğinin belirlenmesi

Year 2021, Volume: 31 Issue: 2, 170 - 174, 15.12.2021

Mürşide Yağcı , Cenk Yücel F. Dolunay Erdoğuş F. Manolya Akbulut

https://doi.org/10.18615/anadolu.1030016

Cited By: 2

Abstract

Patates güvesi Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae), tüm dünyada patates üreten ülkelerde verim kayıplarına neden olan önemli bir patates zararlısıdır. Bu zararlı populasyonu azaltmak için birçok mücadele yöntemi mevcuttur. Bunların başında kimyasal mücadele yöntemleri gelmektedir. Örnek olarak, Entomopatojenik nematodlar (EPN'ler), patates güvesi larvalarını kontrol etmek için kimyasal insektisitlere alternatif ve çevre dostu bir mücadele yöntemi olarak kullanılabilir. Bu çalışmada, Türkiye'deki Heterorhabditis bacteriophora (TOK-20) izolatlarının patates güvesinin son dönem (dördüncü dönem) larvalarına karşı etkinliğinin laboratuvar koşullarında araştırılmasını amaçlanmıştır. Deneyler, steril toprak karışımı ile 150 mililitrelik plastik kaplarda yapılmıştır. Dört nematod konsantrasyonu (0, 250, 500 ve 1000 IJ) doğrudan toprağa uygulanmıştır. Patates güvesi farklı konsantrasyonlarda H. bacteriophora (TOK-20) izolatına duyarlı bulunmuştur. Tüm dozlar kontrolden (su) daha etkili çıkmıştır. En yüksek ölüm oranı en etkili doz olan 1000 IJs konsantrasyonda görülmüş olup, sıcaklıklar arasında herhangi bir istatistiki farklılık görülmemiştir. Bu sonuçlar, H. bacteriophora (TOK-20) izolatının çok etkili olduğunu ve biyolojik mücadele programlarında patates güvesine karşı mücadelede kullanılabileceğini göstermiştir.

Keywords

Entomopatojen nematodlar, Phthorimaea operculella, biyolojik mücadele, etkinlik, patates

Determination of efficiency of entomopathogenic nematode Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) on the potato tuber moth (Phthorimaea operculella (Zeller)) (Lepidoptera: Gelechiidae) under controlled conditions

Abstract

The potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae), is an important pest of potato that causes yield losses in potato producing countries all over the world. Many management methods are available for reducing this pest populations. Chemical control methods are at the top of these. However, Entomopathogenic nematode (EPNs), can be used as a potential alternative to chemical insecticides to control potato tuber moth larvae as an eco-friendly management method. We aimed in this study to examine the efficacy of Turkish isolates of Heterorhabditis bacteriophora (TOK-20) against the last instar larvae (fourth stage) of the potato tuber moth under laboratory conditions. Experiments were conducted in 150 milliliter plastic cups with sterile soil mixture. Four nematode concentrations (0, 250, 500 and 1000 IJs) were applied directly to the soil. Potato tuber moth was susceptible to different concentrations of the H. bacteriophora (TOK-20) isolate. All doses were more effective than the control (water). The most effective was the 1000 IJs concentratio, providing a high mortality rate of the last instar larvae of potato tuber moth. No statistically significant difference was observed among temperatures. These results indicated that this H. bacteriophora (TOK-20) isolate is very efficient and could be used against potato tuber moth in biological control programs.

Keywords

Entomopathogenic nematodes, Phthorimaea operculella, biological control, efficiency, potato

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