Bazı Solanum Türlerinin Clavibacter michiganensis subsp. michiganensis’e Dirençlilik Seviyelerinin Belirlenmesi

Year 2022, Volume: 32 Issue: 1, 115 - 123, 24.06.2022

Utku Şanver , Asena Akköse Baytar Hatice Özaktan Anne Frary Sami Doğanlar

https://doi.org/10.18615/anadolu.1130838

Cited By: 1

Abstract

Clavibacter michiganensis subsp. michiganensis (Cmm), domateste bakteriyel solgunluk ve kansere neden olan yıkıcı bir hastalık ajanıdır. Domateste Cmm’e karşı verim kayıplarını önlemek için kesin bir çözüm yoktur. Ayrıca, günümüz piyasasında Cmm’e karşı dayanıklı, başarılı bir ticari domates çeşidi bulunmamaktadır. Bu nedenle, bu çalışmada bazı domates çeşitlerinin Cmm'ye karşı toleranslık düzeylerini belirlenmesi hedeflenmiştir. Bu önemli hastalığa karşı yeni direnç kaynaklarını belirlemek amacıyla Peru, Ekvador ve Meksika'dan, dört domates türünü (Solanum arcanum, S. habrochaites, S. pennellii ve S. peruvianum) temsil eden yedi domates çeşidi son derece virülent Cmm-244 ve Cmm-9'a karşı kök daldırma yöntemi kullanılarak test edilmiştir. Çalışmamızın sonucunda iki çeşit, S. habrochaites LA1777 ve S. arcanum LA2157, sırasıyla orta ve yüksek toleranslı bulunmuştur ve Türkiye'de domates yetiştiriciliği için önemli tolerans kaynağı olarak kullanılabileceklerdir. Bu malzemeler ayrıca sahip oldukları Cmm tolerans mekanizmalarının ortaya çıkarılması amacı için daha kapsamlı bir şekilde ileriki çalışmalarda araştırılabilir.

Keywords

Solanum spp., yabani türler, bakteriyel kanser ve solgunluk, çeşit dayanıklılığı

Determination of Resistance Levels to Clavibacter michiganensis subsp. michiganensis in Some Solanum Species

Abstract

Clavibacter michiganensis subsp. michiganensis (Cmm), is a devastating bacterial disease agent causing bacterial wilt and canker in tomatoes. There is no definitive solution to prevent yield losses by Cmm in tomatoes. Moreover, there is currently no commercially successful Cmm resistant tomato cultivar on the market. Therefore, we aimed to determine the tolerance level of some tomato accessions to Cmm in the present study. For this purpose, we screened seven tomato accessions representing four species (Solanum arcanum, S. habrochaites, S. pennellii, and S. peruvianum) from Peru, Ecuador, and Mexico against the highly virulent isolates Cmm-244 and Cmm-9. A root immersion method was used to identify new sources of resistance to this important disease. Two accessions, S. habrochaites LA1777, and S. arcanum LA2157 were found to be moderate and highly tolerant, respectively, and could serve as tolerance resources for tomato breeding in Türkiye. These materials can also be investigated more extensively to determine their intrinsic Cmm tolerance mechanism.

Keywords

Solanum spp., wild species, bacterial canker, cultivar resistance

References

• Anonymous. 2021. http://www.fao.org/faostat/en/#data/QC Accessed on 25 June 2021.
• Astua-Monge, G., G. V. Minsavage, R. E. Stall, C. E. Vallejos, M. J. Davis, and J. B. Jones. 2000. Xv4-vrxv4: A New gene-for-gene interaction identified between Xanthomonas campestrispv. vesicatoria race T3 and the wild tomato relative Lycopersicon pennellii. Mol. Plant-Microbe Interact. 13: 1346–1355
• Bai, J., Q. Wei, J. Shu, Z. Gan, B. Li, D. Yan, Z. Huang, Y. Guo, X. Wang, L. Zhang, Y. Cui, X. Lu, J. Lu, C. Pan, J. Hu, Y. Du, L. Liu, and J. Li. 2020. Exploration of resistance to Phelipanche aegyptiaca in tomato. Pest Manag. Sci. 76: 806-3821.
• Balci, E. 2005. Genetic Characterization of Cucumber Mosaic Virus Resistance in Tomato and Pepper. IZTECH MSc Thesis, İzmir
• Baldin, E.L.L., J. D. Vendramin, and A. L. Lourenção. 2005. Resistance of tomato genotypes to the whitefly Bemisia tabaci (Gennadius) biotype B (Hemiptera: Aleyrodidae). Neotrop. Entomol. 34: 435-441.
• Basim, E., H. Basim, E. R. Dickstein and J. B. Jones. 2004. Bacterial canker caused by Clavibacter michiganensis subsp. michiganensis on greenhouse-grown tomato in the Western Mediterranean Region of Turkey. Plant Disease, 88(9): 1048-1048.
• Belgüzar, S., Y. Yanar and Y. Aysan. 2016. Intensity of bacterial wilt disease of tomato in Tokat and identification of disease agent (Clavibacter michiganensis subsp. michiganensis). JAFAG 33: 34-40.
• Bitew, K. M. 2018. Significant role of wild genotypes of tomato trichomes for Tuta absoluta resistance. J. Plant Genet. Breed. 2: 104
• Borba, M. E. A., G. M. Maciel, G. R. Marquez, Jr E. F. Fraga, and G.G.S. Nogueira. 2017. Genetic diversity and selection in tomato genotypes under water stress induced by mannitol. Biosci. J. 33: 592-600.
• Chang, R. J., S. M. Ries, and J. K. Pataky. 1991. Dissemination of Clavibacter michiganensis subsp. michiganensis by practices used to produce tomato transplants. Phytopathol. 81: 1276-1281.
• Chaerani, R., M. J. M. Smulders, C. G. Van Der Linden, B. Vosman, P. Stam, and R. E. Voorrips. 2007. QTL identification for early blight resistance (Alternaria solani) in a Solanum lycopersicum × S. arcanum cross. Theor. Appl. Genet. 114: 439-450.
• Crino, P., P. Veroness, C. Stamigna, D. Chiaretti, A. Lai, M. E. Bitti, F. and Saccardo. 1995. Breeding for resistance to bacterial canker in italian tomatoes for fresh market. Acta Hortic. (ISHS) 412: 539-545.
• El-Halmouch, Y., H. Benharrat, and P. Thalouarn. 2006. Effect of root exudates from different tomato genotypes on broomrape (O. aegyptiaca) seed germination and tubercle development. Crop Prot. 25: 501–507.
• El-Sappah, A.H., M. M. Islam,H. H. El-awady, S. Yan, S. Qi, J. Liu, G. Cheng, and Y. Liang. 2019. Tomato natural resistance genes in controlling the root-knot nematode. Genes 10: 925.
• Fatmi, M., and N.W. Schaad. 2002. Survival of Clavibacter michiganensis ssp. michiganensis in infected tomato stems under natural field conditions in California, Ohio and Morocco. Plant Pathol. 51: 149-154.
• Francis, D. M., E. Kabelka, J. Bell, B. Franchino, and D. St Clair. 2001. Resistance to bacterial canker in tomato (Lycopersicon Hirsutum LA407) and its progeny derived from crosses to L. esculentum. Plant Dis. 85: 1171-1176.
• Frary, A., D. Göl, D. Keleş, B. Ökmen, H. Pınar, H. Ö. Şığva, A. Yemenicioğlu, and S. Doğanlar. 2010. Salt tolerance in Solanum pennellii: antioxidant response and related QTL. BMC Plant Biol. 10: 1-16.
• Gartemann, K.H., O. Kirchner, J. Engemann, I. Gräfen, R. Eichenlaub, and A. Burger. 2003. Clavibacter michiganensis subsp. michiganensis: first steps in the understanding of virulence of a gram-positive phytopathogenic bacterium. J. Biotechnol. 106: 179–191.
• Gordillo, Jr L. F. 2009. Identification and Manipulation of Resistance to Tomato Spotted Wilt Virus Derived from Solanum peruvianum. Theses and Dissertations, 2180. Life Sciences; Plant and Wildlife Sciences, Brigham Young University, Provo. https://scholarsarchive.byu.edu/etd/2180
• Hassan, A.A., D. L. Strider, and T. L. Konsler. 1968. Application of cotyledonary symptoms in screening for resistance of tomato to bacterial canker and host range studied. Phytopathol. 58: 233-239.
• Jablonska, B., J. S. Ammiraju, K. K. Bhattarai, S. Mantelin, O. M. de Ilarduya, P. A. Roberts, and I. Kaloshian. 2007. The Mi-9 gene from Solanum arcanum conferring heat-stable resistance to root-knot nematodes is a homolog of Mi-1. Plant Physiol. 143: 1044-1054.
• Kabas A., Boyaci H.F., Horuz S., Aysan Y., Ilbi H. 2018. Investigation on identification of new resistant resources to bacterial canker and wilt disease. Fresenius Environ. Bull. 27: 8498–8504.
• Kabelka, E., B. Franchino, and D. M. Francis. 2002. Two loci from Lycopersicon hirsutum LA407 confer resistance to strains of Clavibacter michiganensis subsp. michiganensis. Phytopathol. 92: 504–510.
• Kahveci, E ve A. Gürcan. 1993. Antalya İlindeki domates bakteriyel hastalık etmenlerinin tespiti. Bitki Koruma Bülteni. 33:147–151.
• Karaca, İ. ve H. Saygılı. 1982. Batı Anadolunun bazı illerinde domates ve biberde görülen bakteriyel hastalıkların oranı, etmenleri ve konukçu çeşitlerinin duyarlılığı üzerine araştırmalar. III. Türkiye Fitopatoloji Kongresi, 12-15 Ekim, Adana. s. 182–192.
• King, E.O., M. K. Ward, and D. Raney. 1954. Two simple media for the demonstration of Pyocyanin and Fluorescin, J. Lab. Clin. Med. 44: 301-307.
• Klement, Z., K. Rudolph, and D. C. Sands. 1990. Methods in Phytobacteriology. Akademiai Kiado, Budapest, 568.
• Lelis, F.V., R. Czajkowski, R. Souza, D. Ribeiro, and J. Wolf. 2014. Studies on the colonization of axenically grown tomato plants by a GFP tagged strain of C. michiganensis subsp. michiganensis. Eur. J. Plant Pathol. 139: 53-66.
• Li, J., L. Liu, Y. Bai, R. Finkers, F. Wang, Y. Du, Y. Yang, B. Xie, R. G. F. Visser and A. W. van Heusden. 2011. Identification and mapping of quantitative resistance to late blight (Phytophthora infestans) in Solanum habrochaites LA1777. Euphytica 179: 427-438.
• Li, J., L. Liu, Y. Bai, P. Zhang, R. Finkers, Y. Du, R. G. F. Visser, and A. W. van Heusden. 2010. Seedling salt tolerance in tomato. Euphytica, 178: 403–414.
• Maluf, W.R., M. Toma-Braghini, and R. D. Corte. 1991. Progress in breeding tomatoes for resistance to tomato spotted wilt. Rev. Bras. Genét. 14: 509–525.
• Momotaz, A., J. W. Scott, and D. J. Schuster. 2007. Solanum habrochaites accession LA1777 recombinant inbred lines are not resistant to tomato yellow leaf curl virus or tomato mottle virus. HortScience 42: 1149-1152.
• Momotaz, A., J. W. Scott, and D. J. Schuster. 2010. Identification of quantitative trait loci conferring resistance to Bemisia tabaci in an F2 population of Solanum lycopersicum× Solanum habrochaites Accession LA1777. J. Am. Soc. Hortic. Sci. 135: 134-142.
• Oriani, M.A.G., and J.D. Vendramim. 2010. Influence of trichomes on attractiveness and ovipositional preference of Bemisia tabaci (Genn.) B biotype (Hemiptera: Aleyrodidae) on tomato genotypes. Neotrop. Entomol. 39: 1002-1007
• Öktem, Y.E. ve K. Benlioğlu. 1993. Orta Anadolu Bölgesi’nde domates ekim alanlarında bakteriyel hastalıklar üzerine ön çalışmalar. Bitki Koruma Bülteni. 33: 1–6.
• Özyılmaz, Ü. 2001. Aydın İlinde sera domateslerinde toprak kaynaklı bakteriyel hastalıkların saptanması. Yüksek Lisans Tezi. ADÜ. F. B. E. Bitki Koruma Anabilim Dalı, Aydın.
• Özyılmaz, Ü., ve K. Benlioğlu. 2015. Domates bakteriyel kanser hastalığı (Clavibacter michiganensis subsp. michiganensis (smith) davis et al.)’na karşı antagonist bakteriler ile biyolojik mücadele. Doğu Karadeniz 2. Organik Tarım Kongresi. 6-9 Ekim 2015. Rize. s. 96-106.
• Peritore-Galve, F.C., C. Miller, and C. D. Smart. 2019. Characterizing colonization patterns of clavibacter michiganensis during infection of tolerant wild solanum species. Phytopathol. 10: 574-581.
• Peritore-Galve, F. C., M. A. Tancos, and C. D. Smart. 2021. Bacterial canker of tomato: revisiting a global and economically damaging seedborne pathogen. Plant Dis. PDIS08201732FE. doi:10.1094/pdis-08-20-1732-fe
• Pine, T.S., R. G. Grogan, and W. B. Hewıtt. 1954. Pathological anatomy of bacterial canker of young tomato plants. Phytopathol. 45: 267-271.
• Poysa, V. 1993. Evaluation of tomato breeding lines resistant to bacterial canker. Can. J. Plant Pathol. 15: 301–304.
• Razdan, M. K., and A. K. Mattoo. (Eds.) 2007. Genetic Improvement of Solanaceous Crops: Tomato, 2, Science Publishers, Inc., Enfield, NH.
• RStudio Team 2020. RStudio: Integrated Development for R. RStudio, PBC, Boston, MA URL http://www.rstudio.com/.
• Sandbrink, J. M., J. W. van Ooijen, C. C. Purimahua, M. Vrielink, R. Verkerk, P. Zabel, and P. Lindhout. 1995. Localization of genes for bacterial canker resistance in Lycopersicon peruvianum using RFLPs. Theor. Appl. Genet. 90: 444–450.
• Scott, J.W. and Jones, J.P. 1989. Monogenic resistance in tomato to Fusarium oxysporum f. sp. lycopersici race 3. Euphytica 40: 49-53.
• Sen, Y., Z. Feng, H. Vandenbroucke, J. van der Wolf, R.G.F. Visser, and A.W. van Heusden. 2012. Screening for new sources of resistance to Clavibacter michiganensis subsp. michiganensis (Cmm) in tomato. Euphytica 190: 309–317.
• Seifi, A., I. Kaloshian, J. Vossen, D. Che, K. K. Bhattarai, J. Fan, Z. Naher, A. Goverse, W. F. Tjallingii, P. Lindhout, R. G. F. Visser, and Y. Bai. 2011. Linked, if not the same, mi-1 homologues confer resistance to tomato powdery mildew and root-knot nematodes. Mol. Plant-Microbe Interact. 24: 441-450.
• Sotirova, V., N. Bogatsevska, and L. Stamova. 1994.Sources of resistance to bacterial diseases in tomato wild species. Acta Hortic. 376: 353-359.
• Thapa, S.P., E. M. Miyao, R. M. Davis, and G. Coaker. 2015. Identification of QTLs controlling resistance to Pseudomonas syringae pv. tomato race 1 strains from the wild tomato, Solanum habrochaites LA1777. Theor. Appl. Genet. 128: 681-692.
• Toprak, F.C., E. Barutcu, A. Frary, and S. Doganlar. 2009. Identification of Potato Y Potyvirus (PVY^ O) resistance in wild and cultivated tomatoes. TJAF 33 (1): 11-17.
• Townsend, G.R., and J. W. Heuberger. 1943. Methods for estimating losses caused by diseases in fungicide experiments. Plant Dis. Rep. 27: 340-343.
• Thyr, B. D. 1976. Inheritance of Resistance to Corynebacterium michiganense in Tomato. Phytopathol. 66: 1116–19.
• Uyar, S. 2011. Akdeniz bölgesinde örtüalti domates üretiminde kullanilan yaygin domates çeşitlerinin domates bakteriyel solgunluk ve kanser hastalik etmeni (Clavibacter michiganensis subsp. michiganensis)’e karşı dayanaklılık reaksiyonlarının belirlenmesi. Akdeniz University, MSc Thesis, Faculty of Science, Antalya.
• Veremis, J. C., and P. A. Roberts. 2000. Diversity of heat-stable genotype specific resistance to Meloidogyne in Maranon races of Lycopersicon peruvianum complex. Euphytica 111: 9–16.
• Yang, R., J. Cheng, C. Zhang, and S. Wang. 2011. Selection of tomato cultivars with resistance to root knot nematodes. international conference on remote sensing. Environ. and Transp. Eng. 7807-7810.
• Wittmann, J., C. Brancato, K. W. Berendzen, and B. Dreiseikelmann. 2015. Development of a Tomato Plant Resistant to Clavibacter michiganensisusing the Endolysin Gene of Bacteriophage CMP1 as a Transgene. Plant Pathol. 65: 496–502.
• Yıldız, R.Ç. ve Y. Aysan. 2008. Domates Bakteriyel Solgunluk Hastalığı Etmeni (Clavibacter michiganensis subsp. michiganensis (Smith) Davis et. al.)’nin İzolasyonu Geleneksel, Serolojik ve Moleküler Yöntemlerle Tanılanması. Ç.Ü. Fen Bilim. Enstitüsü. 19:113– 122.
• Yuqing, W., Z. Yaxian, G. Zhipeng, and Y. Wencai. 2018. Breeding for resistance to tomato bacterial diseases in China: Challenges and prospects. Hortic. Plant J. 4: 193-207.