Farklı Lokasyonlardan Toplanan Salvia aramiensis Rech. f. Tohumları Üzerine Önişlem Uygulamalarının Etkisi

Year 2024, Volume: 34 Issue: 1, 70 - 81, 30.06.2024

Nadire Pelin Bahadırlı

https://doi.org/10.18615/anadolu.1453157

Abstract

Salvia aramiensis Rech. f. Türkiye'nin Hatay ilinde yerel endemik olan Lamiaceae familyasına ait çok yıllık bir yarı çalıdır. Çalışmada S. aramiensis tohumlarının çimlenme parametreleri üzerine lokasyon ve priming etkilerinin değerlendirilmesi amaçlanmıştır. Hatay'ın altı ilçesinde (Antakya, Arsuz, Belen, Dörtyol, Samandağ ve Yayladağı) araştırma yapılmış ve 2020 yazında ayrı ayrı tohumlar toplanmıştır. Tohum nemi (%) ve bin tane ağırlığı (TSW-g) belirlenmiştir. Çalışma kapsamında 33 adet önişlem tohumlara uygulanmış ancak sadece 10 önişlem uygulamasında çimlenme tespit edilmiştir. Çalışmada çimlenme oranı (%), ortalama çimlenme süresi (MGT), çimlenme indeksi (GI) ve T50 belirlenmiştir. Çimlenme parametreleri üzerine lokasyon ve priminglerin istatistiksel olarak önemli (p<0,05) olduğu belirlenmiştir. Tohum nemleri %5,76-7,87 aralığında, TSW ise 5,77-10,05 g arasında değişmiştir. En yüksek çimlenme oranı %65,50 ile Belen ilçesi tohum örneklerinde 30d soğuk katlama/2000ppm GA3 uygulaması kombinasyonunda gözlenmiştir. MGT 2,87-7,71 gün arasında değişmekte olup en yüksek sonuç Antakya ilçesinden elde edilmiştir. GI sonuçları 0,42-11,30, T50 sonuçları ise 1,82-6,94 gün arasında değişmiştir. Bu çalışmada Belen lokasyonu incelenen parametrelere göre önem göstermiştir.

Keywords

Çimlenme, adaçayı, dormansi, katlama, hormon

Project Number

20-M-038

Priming Effects on Germination Parameters of Vulnerable Salvia aramiensis Rech. f. Harvested from Different Locations

Abstract

Salvia aramiensis Rech. f. is a perennial sub-shrub from the Lamiaceae family that is locally endemic in the Hatay province of Türkiye. The study aimed to evaluate the location and priming effects on germination parameters of S. aramiensis seeds. Six districts of Hatay (Antakya, Arsuz, Belen, Dörtyol, Samandağ, and Yayladağı) were surveyed and seeds were collected separately during summer 2020. Seed moisture (%) and thousand seed weight (TSW-g) were determined. A total of 33 priming applications were applied and control groups (no treatment) were included, however, only ten of them showed germination. Germination rate (%), mean germination time (MGT), germination index (GI), and T50 were determined. Locations and priming were found to be statistically significant (p<0.05) on germination parameters. The seed moistures ranged between 5.76 and 7.87% and the TSW seed varied between 5.77 and 10.05g. The highest germination rate was observed in Belen district seed samples as 65.50% with 30 d cold stratification/2000 ppm GA3 treatment combination. MGT ranged between 2.87 and 7.55 days and the highest result was obtained from Antakya district. GI results ranged between 0.42 and 11.30 and T50 results ranged between 1.82 and 6.94 days. In the present study, Belen location showed importance according to the studied parameters.

Keywords

Germination, sage, dormancy, stratification, hormone

Project Number

20-M-038

References

• Abdollahi, J., M. Ebrahimi, H.A. Ramshini, M. Eftekhari, Y.S. Mansouri, and M.A. Sheikh-Beig-Goharrizi. 2012. Seed germination as the major conservation issue of endemic Iranian Salvia species. J. Med. Plants Res. 6 (1): 37-46.
• Alim, G. O., T. Yilmaz Ozden, S. Yazici Tutunis, N. Tan, and M. Miski. 2018. Anti-alzheimer activity of Salvia aramiensis root extract 12th International Symposium of Pharmaceutical Sciences (ISOPS-12). 26-29 June. 160-161. Ankara, Turkey.
• Al-Gharaibeh, M. M., H. R. Hamasha, S. Lachmuth, and I. Hensen. 2017. Local adaptation to different phytogeographic regions: habitat-related variations in seed germination in response to temperature and salinity for two medicinal Salvia species from Jordan. Plant Species Biology 32: 25-35. https://doi.org/10.1111/1442-1984.12123.
• AOSA, and SCST. 1993. Rules for Testing Seeds. Journal of Seed Technology 16: 1–113.
• Ayanoğlu, F., D. A. Kaya, A. Mert and V. Uygur. 2012. Hatay yöresinde doğal olarak yetişen adaçayı (Salvia sp.) türlerinin kültüre alınması, verim ve kalite özelliklerinin belirlenmesi. TÜBİTAK TOVAG, Proje No: 190O161, 152 p.
• Bahadirli, N. P., and F. Ayanoglu, 2019. Preliminary results: assessment of new Salvia chemotypes for herbal tea industry by hybridization. Scientific Papers. Series A: Agronomy 62 (1): 501–506.
• Bahadirli, N. P. 2020. Economically important sage species from Turkey: Salvia fruticosa Mill. and S. aramiensis Rech fil. Current Perspectives on Medicinal and Aromatic Plants 3 (1): 31–42. https://doi.org/10.38093/cupmap.736482.
• Bahadirli, N. P., and F. Ayanoglu. 2021. Genetic diversity of Salvia species from Turkey assessed by microsatellite markers. Journal of Applied Research on Medicinal and Aromatic Plants 20: 100281. doi: 10.1016/j.jarmap.2020.100281.
• Bahadirli, N. P. 2022. Comparison of chemical composition and antimicrobial activity of Salvia fruticosa Mill. and S. aramiensis Rech. Fill. (Lamiaceae). Journal of Essential Oil Bearing Plants 25 (4): 716–727. https://doi.org/10.1080/0972060X.2022.2110388.
• Caliskan, O., K. Mavi, and A. Polat. 2012. Influences of presowing treatments on the germination and emergence of fig seeds (Ficus carica L.). Acta Scientiarum Agronomy 34(3): 293-297. Claßen-Bockhoff, R., T. Speck, E. Tweraser, P. Wester, S. Thimm, and M. Reith. 2004. The staminal lever mechanism in Salvia L. (Lamiaceae): a key innovation for adaptive radiation? Organisms, Diversity & Evolution 4: 189-205.
• Celep, F., M., Doğan, and A. Kahraman. 2010. Re-evaluated conservation status of Salvia L. (sage) in Turkey I: The Mediterranean and the Aegean geographic regions. Turkish Journal of Botany 34: 201-214. https://doi.org/10.3906/bot-0902-17.
• Coolbear, P., A. Francis, D. Grierson. 1984. The effect of low temperature pre-sowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds. Journal of Experimental Botany 35 (160): 1609-1617. https://doi.org/10.1093/jxb/35.11.1609.
• Dastanpoor, N., H. Fahimi, M. Shariati, S. Davazdahemami, and S. M. M. Hashemi. 2013. Effects of hydropriming on seed germination and seedling growth in sage (Salvia officinalis L.). African Journal of Biotechnology 12 (11): 1223-1228.
• Davis, P. H. 1982. Flora of Turkey and The East Aegeans Islands (Vol: 1-11). The Edinburg University Press. ISBN: 9780852243961, England.
• Demirci, B., K. H. C. Başer, and G. Tümen. 2002. Composition of the essential oil of Salvia aramiensis Rech. Fil. growing in Turkey. Journal of Flavour and Fragrance 17: 23-25. https://doi.org/10.1002/ffj.1027.
• De Paiva, E. P., S. B. Torres, F. V. da Silva Sá, N. W. Nogueira, R. M. O de Freitas, and M. de Sousa Leite. 2016. Light regime and temperature on seed germination in Salvia hispanica L. Acta Scientiarum 38 (4): 513–519.
• Ellis, R. H. and E. H. Roberts. 1980 Towards rational basis for testing seed quality. In: P. D. Hebblethwaite (Ed.). Seed Production. 605-635. Butterworths, London.
• Ellis, R. H., T. D. Hong and E. H. Roberts. 1985a. Handbook of Seed Technology for Genebanks - Volume I. Principles and Methodology. International Board for Plant Genetic Resources, ISBN No. 92-9043-118-0, 225 p.
• Ellis, R. H., T. D. Hong, E. H. Roberts. 1985b. Handbook of Seed Technology for Genebanks - Volume II. Compendium of Specific Germination Information and Test Recommendations. Handbooks for Genebanks No:3, ISBN No. 91-9043-119-9, p 675.
• Ertaş, A., H. Alkan, M. Akdeniz, H. Şahin, I. Alacabey, M. Odabaşı, O. Acet, and U. Kolak. 2017. The antioxidant activity and chemical composition of essential oils of Salvia aramiensis and Calamintha nepeta. p. 612. In: The 3rd International Symposium on EuroAsian Biodiversity, 05-08 July 2017, Minsk, Belarus.
• Gorai, M., H. Gasmi and M. Neffati. 2011. Factors influencing seed germination of medicinal plant Salvia aegyptica L. (Lamiacea). Saudi Journal of Biological Sciences 18: 255-260. https://doi.org/10.1016/j.sjbs.2011.01.004.
• Guner, A., S. Aslan, T. Ekim, M. Vural, and M. T. Babaç. 2012. Türkiye Bitkileri Listesi (Damarlı Bitkiler). Flora Araştırmaları Derneği ve Nezahat Gökyiğit Botanik Bahçesi Yayını, ISBN: 9786056042577, 610 s, İstanbul.
• Güzel Y, and Güzelşemme M. 2018. Wild plants used as herbal tea in Antakya and Defne Provinces of Hatay. ANADOLU 28 (1): 1-5.
• ISTA (International Seed Testing Association).1985. International Rules for Seed Testing. Seed Science and Technology. 13: 299-513.
• Ixtaina, V. Y., S. M. Nolasco, and M. C. Tomás. 2008. Physical properties of chia (Salvia hispanica L.) seeds. Industrial Crops and Production 28: 286–293.
• Javaid, M. M., S. Florentine, H. H. Ali and S. Weller. 2018. Effect of environmental factors on the germination and emergence of Salvia verbenaca L. cultivars (verbenaca and vernalis): An invasive species in semi-arid and arid rangeland regions. Plos One 13 (3): e0194319. https://doi.org/10.1371/journal.pone.0194319.
• Karaman, Ş., A. İlçim and N. Çömlekçioğlu. 2007. Composition of the essential oils of Salvia aramiensis Rech. Fil. and Salvia cyanescens BOISS. & BAL. Pakistan Journal of Botany 39 (1): 169-172. https://doi.org/10.1002/ffj.1027. Kretschmer, M. 1989. Influence of different storage conditions on germination of spice seeds. Acta Horticulturae 253: 99-105. https://doi.org/10.17660/ActaHortic.1989.253.9.
• Lacey, E. P., and D. Herr. 2000. Parental effects in Plantago lanceolata L. III. measuring parental temperature effects in the field. Evolution 54 (4): 1207–1217. https://doi.org/10.1111/j.0014-3820.2000.tb00555.x
• Liu, L., Q. S. Guo, Y. P. Wang, and R. M. Zhao. 2006. Study on characteristics of seed germination of Salvia officinalis. China Journal of Chinese Materia Medica 31: 1587-9.
• Ozcan, I. I., O. Arabaci and N. G. Ogretmen. 2014. Bazı adaçayı türlerinde farklı tohum çimlendirme uygulamalarının belirlenmesi. Türk Tarım Gıda Bilim ve Teknolojileri Dergisi 2 (5): 203-207.
• Ryding, O. 2001. Myxocarpy in the Nepetoideae (Lamiaceae) with notes on myxodiaspory in general. Systematic Geography of Plants 71: 503–514.
• Snedecor, G.W. and W.G. Cochran. 1989. Statistical Methods. 8th Edition, Iowa State University Press, Ames.
• Sonmez, C., A. Gokcol, A. Şimşek Soysal, E. Bayram and A. Celen. 2019. Adaçayı (Salvia spp.) türlerinde çimlenme ve çıkış performansını arttırıcı uygulamalar. Turkish Journal of Agriculture Food Science and Technology 7: 504-510.
• Thanos, C. A. 1993. Germination ecophysiology of Mediterranean aromatic plants. D. Come & F. Corbineau (eds), 4th International Workshop on Seeds. Basic and Applied Aspects of Seed Biology. 1: 281-287.
• Tursun, A. O. 2019. Salvia verticillata L. (Dadırak)'nın tohum dormansisinin kırılmasında farklı uygulamaların etkileri. KSÜ Tarım ve Doğa Dergisi 22 (Ek sayı 1): 30-37. https://doi.org/10.18016/ksutarimdoga.vi.560605.
• Yenikalayci, A. 2022. Cultivation possibilities of Salvia aramiensis in the continental and the mediterranean type of climate. Emirates Journal of Food and Agriculture 34 (7): 628-633. https://doi.org/10.9755/ejfa.2022.v34.i7.2889.
• Zona, S. 2017. Fruit and seed dispersal of Salvia L. (Lamiaceae): a review of the evidence. Botanival Reviews 83: 195–212 (2017). https://doi.org/10.1007/s12229-017-9189-y