Farklı Bor Tuzları ile Muamele Edilen Olea europaea L.'nin In Vitro Kültürlerinde ROS Genlerinin mRNA Transkripsiyon Analizleri

Year 2024, Volume: 34 Issue: 1, 24 - 32, 30.06.2024

Onur Çelik , Ergun Kaya

https://doi.org/10.18615/anadolu.1457244

Abstract

Biyotik ve abiyotik stresler gibi çeşitli faktörler bitki metabolizması, gelişimi ve büyümesi üzerinde etkilidir. Bitkiler tüm bu stres koşullarına uyum sağlamak, savunmak, kaçınmak ve tolere etmek için birçok karmaşık ve olağanüstü stratejiye sahiptir. Bu çalışmada, in vitro koşullarda çoğaltılması oldukça zor olan zeytinin antioksidan enzimlerinin oksidatif stres koşullarında, bor bileşikleri sonrası göreceli mRNA seviyeleri incelenmiştir. Bu kapsamda, askorbat-glutatyon yolağını etkilediği bilinen bor elementinin üç farklı bileşiği, in vitro koşullarda zeytinin besin ortamına iki farklı konsantrasyonda ayrı ayrı ilave edildi. Çalışma sonucunda katalaz, askorbat peroksidaz ve süperoksit dismutaz gibi antioksidan enzimlerin rölatif mRNA ekspresyon seviyelerinin deney grupları arasında sadece H3BO3 grubunda azaldığı gözlendi. Kontrol grubuna kıyasla NaBO2 ve ZnBO3 gruplarında antioksidan enzimlerin rölatif mRNA ekspresyon seviyelerinde artış gözlenmiştir. Bu durum, uygulanan NaBO2 ve ZnBO3 gruplarında tuzluluk stresinin ve dolayısıyla oksidatif stresin arttığı şeklinde yorumlanmıştır. Ancak H3BO3 grubunda konsantrasyon iki kat artmasına rağmen antioksidan enzimlerin göreceli mRNA ifade seviyelerinde düşüş gözlenmiştir.

Keywords

Askorbat peroksidaz, borik asit, katalaz, sodyum metaborat, süperoksit dismutaz

Supporting Institution

This study was supported by The Scientific and Technological Research Council of Türkiye 2210-C (TUBITAK 2210-C) Domestic Priority Areas Graduate Scholarship Program

Thanks

The authors would like to thank Muğla Metropolitan Municipality Agricultural Services Department for support of plant materials

mRNA Transcription Analyses of ROS Genes of Olea europaea L. In Vitro Cultures Treated with Different Boron Salts

Abstract

Various factors such as biotic and abiotic stresses have effects on plant metabolism, development, and growth. Plants have many complex and extraordinary strategies to adapt, defend, avoid and tolerate all these stress conditions. In this study, the relative mRNA levels of antioxidant enzymes of olive, which is very difficult to reproduce under in vitro conditions, were assessed under oxidative stress conditions, after treatment with boron compounds. In this context, three different compounds of the element boron, which are known to affect the ascorbate-glutathione pathway, were added separately at two different concentrations to the nutrient medium of olive under in vitro conditions. As a result of the study, it was observed that the relative mRNA expression levels of antioxidant enzymes such as catalase, ascorbate peroxidase, and superoxide dismutase decreased only in the H3BO3 group among the experimental groups. An increase in the relative mRNA expression levels of antioxidant enzymes was observed in the NaBO2 and ZnBO3 groups compared to the control group. This situation was interpreted as due to an increase in salinity stress which thereby increased the oxidative stress of the applied NaBO2 and ZnBO3 groups. However, in the H3BO3 group, although the concentration was increased twofold, a decrease was observed in the relative mRNA expression levels of the antioxidant enzymes examined. This reveals that application concentration, as well as the compound used, is extremely important.

Keywords

Ascorbate peroxidase, boric acid, catalase, sodium metaborate, superoxide dismutase

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