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The Effects of Different Photoactive Dyes on the Adhesion of an Epoxy Resin-based Root Canal Sealer

Year 2021, Volume: 26 Issue: 3, 225 - 232, 27.09.2021
https://doi.org/10.21673/anadoluklin.815730

Abstract

Aim: In this study, we aimed to evaluate the effects of four different photoactive dyes on the dentin adhesion of an epoxy resin-based root canal sealer.
Methods: Sixty maxillary central incisors were used. Root canals were prepared up to the size Reciproc #40, and 2.5 mL of 5% NaOCl was used between each file. The teeth were divided into six groups: Group 1, distilled water (the control group); Group 2, methylene blue; Group 3, toluidine blue; Group 4, indocyanine green; Group 5, Congo red; and Group 6, diode laser. After final irrigation, each canal was irrigated with 2.5 mL of photoactive dye. Laser irradiation was applied for 1 min (940 nm wavelength, continuous mode, 1W). The canals were obturated with epoxy resin-based sealer and gutta-percha. Sections measuring 2 mm in thickness were taken from the apical, middle, and coronal thirds of the roots for the push-out test.
Results: Although the photoactive dyes caused a decrease in the sealer bond strength values, there was no statistically significant difference from the control group or between the dyes. Diode laser irradiation increased bond strength, but there was again no significant difference. Bond strength values decreased from the coronal to the apical region (5.89±1.19, 3.93±0.76, and 3.18±0.63 MPa, respectively).
Conclusion: We observed that the use of photoactive dyes to support root canal disinfection had no negative effect on the bond strength of a resin-based sealer.

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References

  • Peters OA, Schonenberger K, Laib A. Effects of four Ni-Ti preparation techniques on root canal geometry assessed by microcomputed tomography. Int Endod J. 2001;34(3):221–30.
  • Ghoneim AG, Lutfy RA, Sabet NE, Fayyad DM. Resistance to fracture of roots obturated with novel canal‐filling systems. J Endod. 2011;37(11):1590–2.
  • Branstetter J, von Fraunhofer JA. The physical properties and sealing action of endodontic sealer cements: a review of the literature. J Endod. 1982;8(7):312–6.
  • Osiri S, Banomyong D, Sattabanasuk V, Yanpiset K. Root reinforcement after obturation with calcium silicate‐based sealer and modified gutta‐percha cone. J Endod. 2018;44(12):1843–8.
  • Keine KC, Kuga MC, Tormin FBC, Venção AC, Duarte MAH, Chávez-Andrade GM, et al. Effect of peracetic acid used as single irrigant on the smear layer, adhesion, and penetrability of AH Plus. Braz Oral Res. 2019;33:e057.
  • Menezes M, Prado M, Gomes B, Gusman H, Simão R. Effect of photodynamic therapy and non-thermal plasma on root canal filling: analysis of adhesion and sealer penetration. J Appl Oral Sci. 2017;25(4):396–403.
  • Gonçalves L, Silva-Sousa YTC, Neto WR, Teixeira CS, Sousa-Neto MD, Alfredo E. Effect of different irrigation protocols on the radicular dentin interface and bond strength with a metacrylate-based endodontic sealer. Microsc Res Tech. 2014;77(6):446–52.
  • Ayrancı LB, Köseoğlu M. The evaluation of the effects of different irrigating solutions and laser systems on adhesion of resin-based root canal sealers. Photomed Laser Surg. 2014;32(3):152–9.
  • Flores DS, Rached FJ Jr, Versiani MA, Guedes DF, Sousa-Neto MD, Pécora JD. Evaluation of physicochemical properties of four root canal sealers. Int Endod J. 2011;44(2):126–35.
  • Torres FFE, Zordan-Bronzel CL, Guerreiro-Tanomaru JM, Chávez-Andrade GM, Pinto JC, Tanomaru-Filho M. Effect of immersion in distilled water or phosphate-buffered saline on the solubility, volumetric change and presence of voids within new calcium silicate-based root canal sealers. Int Endod J. 2020;53(3):385–91.
  • Lin J, Shen Y, Haapasalo M. A comparative study of biofilm removal with hand, rotary nickel-titanium, and self-adjusting file instrumentation using a novel in vitro biofilm model. J Endod. 2013;39(5):658–63.
  • Navabi AA, Khademi AA, Khabiri M, Zarean P, Zarean P. Comparative evaluation of Enterococcus faecalis counts in different tapers of rotary system and irrigation fluids: an ex vivo study. Dent Res J. 2018;15(3):173–9.
  • Plotino G, Cortese T, Grande NM, Leonardi DP, Di Giorgio G, Testarelli L, et al. New technologies to improve root canal disinfection. Braz Dent J. 2016;27(1):3–8.
  • Gursoy H, Ozcakir-Tomruk C, Tanalp J, Yilmaz S. Photodynamic therapy in dentistry: a literature review. Clin Oral Investig. 2013;17(4):1113–25.
  • Chrepa V, Kotsakis GA, Pagonis TC, Hargreaves KM. The effect of photodynamic therapy in root canal disinfection: a systematic review. J Endod. 2014;40(7):891–8.
  • Foote CS. Definition of type I and type II photosensitized oxidation. Photochem Photobiol. 1991;54(5):659.
  • Konopka K, Goslinski T. Photodynamic therapy in dentistry. J Dent Res. 2007;86(8):694–707. Karadas M, Demirbuga S. Influence of a short-time antioxidant application on the dentin bond strength after intracoronal bleaching. Microsc Res Tech. 2019;82(10):1720–7.
  • Harrison MS, Wang Y, Frick KJ, Moniz J, Walker MP. Effects of alpha-tocopherol antioxidant on dentin-composite microtensile bond strength after sodium perborate bleaching. J Endod. 2019;45(8):1053–9.
  • Yavari H, Ghasemi N, Divband B, Rezaei Y, Jabbari G, Payahoo S. The effect of photodynamic therapy and polymer solution containing nano-particles of Ag/ZnO on push-out bond strength of the sealers AH-Plus and MTA Fillapex. J Clin Exp Dent. 2017;9(9):e1109–14.
  • Banci HA, Strazzi-Sahyon HB, Duarte M, Cintra L, Gomes-Filho JE, Chalub LO, et al. Influence of photodynamic therapy on bond strength and adhesive interface morphology of MTA based root canal sealer to different thirds of intraradicular dentin. Photodiagnosis Photodyn Ther. 2020;32:102031.
  • Camacho-Alonso F, Julián-Belmonte E, Chiva-García F, Martínez-Beneyto Y. Bactericidal efficacy of photodynamic therapy and chitosan in root canals experimentally infected with Enterococcus faecalis: an in vitro study. Photomed Laser Surg. 2017;35(4):184–9.
  • Katalinić I, Budimir A, Bošnjak Z, Jakovljević S, Anić I. The photo-activated and photo-thermal effect of the 445/970 nm diode laser on the mixed biofilm inside root canals of human teeth in vitro: a pilot study. Photodiagnosis Photodyn Ther. 2019;26:277–83.
  • Zorita-García M, Alonso-Ezpeleta LÓ, Cobo M, Del Campo R, Rico-Romano C, Mena-Álvarez J, et al. Photodynamic therapy in endodontic root canal treatment significantly increases bacterial clearance, preventing apical periodontitis. Quintessence Int. 2019;50(10):782–9.
  • Neelakantan P, Sharma S, Shemesh H, Wesselink PR. Influence of irrigation sequence on the adhesion of root canal sealers to dentin: a Fourier transform infrared spectroscopy and push-out bond strength analysis. J Endod. 2015;41(7):1108–11.
  • Ramos ATPR, Belizário LG, Venção AC, Jordão-Basso KCF, Rastelli ANS, Andrade MF, et al. Effects of photodynamic therapy on the adhesive interface of fiber posts cementation protocols. J Endod. 2018;44(1):173–8.
  • Marciano MA, Bramante CM, Duarte MA, Delgado RJ, Ordinola-Zapata R, Garcia RB. Evaluation of single root canals filled using the lateral compaction, Tagger’s hybrid, microseal and guttaflow techniques. Braz Dent J. 2010;21(5):411–5.
  • Fickweiler S, Szeimies RM, Bäumler W, Steinbach P, Karrer S, Goetz AE, et al. Indocyanine green: intracellular uptake and phototherapeutic effects in vitro. J Photochem Photobiol B. 1997;38(2–3):178–83.
  • Saha B, Chowdhury S, Sanyal D, Chattopadhyay K, Kumar GS. Comparative study of toluidine blue O and methylene blue binding to lysozyme and their inhibitory effects on protein aggregation. ACS Omega. 2018;3(3):2588–601.
  • Nagai Y, Suzuki A, Katsuragi H, Shinkai K. Effect of antimicrobial photodynamic therapy (aPDT) on the sterilization of infected dentin in vitro. Odontology. 2018;106(2):154–61.
  • Ok E, Ertas H, Saygili G, Gok T. Effect of photoactivated disinfection on bond strength of root canal filling. J Endod. 2013;39(11):1428–30.
  • Tilakchand M, Singh NN, Yeli MM, Naik BD. Evaluation of the antibacterial efficacy of EZLASE diode LASER on the infected root canal system: an in vivo study. J Conserv Dent. 2018;21(3):306–10. Namvar MA, Vahedi M, Abdolsamadi HR, Mirzaei A, Mohammadi Y, Jalilian FA. Effect of photodynamic therapy by 810 and 940 nm diode laser on herpes simplex virus 1: an in vitro study. Photodiagnosis Photodyn Ther. 2019;25:87–91.
  • Aytac Bal F, Ozkocak I, Cadirci BH, Karaarslan ES, Cakdinleyen M, Agaccioglu M. Effects of photodynamic therapy with indocyanine green on Streptococcus mutans biofilm. Photodiagnosis Photodyn Ther. 2019;26:229–34.
  • Sen OG, Kaya M. Effect of root canal disinfection with a diode laser on postoperative pain after endodontic retreatment. Photobiomodul Photomed Laser Surg. 2019;37(2):85–90.
  • Osmari D, Ferreira ACO, Bello MC, Susin AH, Aranha ACC, Marquezan M, et al. Micromorphological evaluation of dentin treated with different desensitizing agents. J Lasers Med Sci. 2013;4(3):140–6.
  • Yilmaz Y, Keles S, Mete A. Temperature changes in the pulpal chamber and the sealing performance of various methods of direct pulp capping of primary teeth. Eur J Paediatr Dent. 2013;14(2):95–100.
  • Kasraei S, Yarmohamadi E, Jahromi PR, Akbarzadeh M. Effect of 940nm diode laser irradiation on microtensile bond strength of an etch and rinse adhesive (Single Bond 2) to dentin. J Dent. 2019;20(1):30–6.
  • Pantoja CAMS, Silva DHD, Soares AJ, Ferraz CCR, Gomes BPFA, Zaia AA, et al. Influence of ethanol on dentin roughness, surface free energy, and interaction between AH Plus and root dentin. Braz Oral Res. 2018;32:e33.
  • Swift EJ Jr. Critical appraisal. Reversal of compromised bonding after bleaching. J Esthet Restor Dent. 2012;24(5):357–61. Titley KC, Torneck CD, Ruse ND, Krmec D. Adhesion of a resin composite to bleached and unbleached human enamel. J Endod. 1993;19(3):112–5.
  • Santos GC, Baia JC, Ribeiro ME, Lima RR, Sousa Júnior MHSE, Loretto SC. Influence of prolonged bleaching with 4% hydrogen peroxide containing calcium and different storage times on the bond strength to enamel. J Contemp Dent Pract. 2019;20(2):216–20.

Farklı Fotoaktif Boyaların Epoksi Rezin Esaslı Kök Kanal Patının Adezyonuna Etkileri

Year 2021, Volume: 26 Issue: 3, 225 - 232, 27.09.2021
https://doi.org/10.21673/anadoluklin.815730

Abstract

Amaç: Bu çalışmada dört farklı fotoaktif boyanın epoksi rezin esaslı bir kök kanal patının dentin adezyonu üzerindeki etkilerini değerlendirmek amaçlanmıştır.
Yöntem: Altmış maksiller santral kesici diş kullanıldı. Reciproc #40 boyutuna kadar kök kanalları şekillendirildi ve her eğe arasında 2,5 mL %5 NaOCl kullanıldı. Dişler altı gruba ayrıldı: Grup 1, distile su (kontrol grubu); Grup 2, metilen mavisi; Grup 3, toluidin mavisi; Grup 4, indosiyanin yeşili; Grup 5, Kongo kırmızısı; ve Grup 6, diyot lazer. Son irigasyondan sonra her kanal 2,5 mL fotoaktif boya ile yıkandı. Bir dakika boyunca (940 nm dalga boyu, sürekli mod, 1W) lazer ışını uygulandı. Kanallar epoksi rezin esaslı pat ve gütaperka ile dolduruldu. İtme testi için köklerin apikal, orta ve koronal üçte birlik kısımlarından 2 mm kalınlığında kesitler alındı.
Bulgular: Fotoaktif boyalar pat adezyon değerlerinde düşüşe neden olsa da, kontrol grubuna kıyasla ya da boyalar arasında istatistiksel olarak anlamlı bir fark yoktu. Diyot lazer ışınlaması adezyonu artırdı; ancak yine anlamlı bir fark yoktu. Bağlanma değerleri koronalden apikal bölgeye azaldı (sırasıyla 5,89±1,19, 3,93±0,76 ve 3,18±0,63 MPa).
Sonuç: Kök kanal dezenfeksiyonunu desteklemek için fotoaktif boyaların kullanımının rezin esaslı patın adezyonu üzerinde olumsuz bir etkisi olmadığı gözlenmiştir.

Project Number

-

References

  • Peters OA, Schonenberger K, Laib A. Effects of four Ni-Ti preparation techniques on root canal geometry assessed by microcomputed tomography. Int Endod J. 2001;34(3):221–30.
  • Ghoneim AG, Lutfy RA, Sabet NE, Fayyad DM. Resistance to fracture of roots obturated with novel canal‐filling systems. J Endod. 2011;37(11):1590–2.
  • Branstetter J, von Fraunhofer JA. The physical properties and sealing action of endodontic sealer cements: a review of the literature. J Endod. 1982;8(7):312–6.
  • Osiri S, Banomyong D, Sattabanasuk V, Yanpiset K. Root reinforcement after obturation with calcium silicate‐based sealer and modified gutta‐percha cone. J Endod. 2018;44(12):1843–8.
  • Keine KC, Kuga MC, Tormin FBC, Venção AC, Duarte MAH, Chávez-Andrade GM, et al. Effect of peracetic acid used as single irrigant on the smear layer, adhesion, and penetrability of AH Plus. Braz Oral Res. 2019;33:e057.
  • Menezes M, Prado M, Gomes B, Gusman H, Simão R. Effect of photodynamic therapy and non-thermal plasma on root canal filling: analysis of adhesion and sealer penetration. J Appl Oral Sci. 2017;25(4):396–403.
  • Gonçalves L, Silva-Sousa YTC, Neto WR, Teixeira CS, Sousa-Neto MD, Alfredo E. Effect of different irrigation protocols on the radicular dentin interface and bond strength with a metacrylate-based endodontic sealer. Microsc Res Tech. 2014;77(6):446–52.
  • Ayrancı LB, Köseoğlu M. The evaluation of the effects of different irrigating solutions and laser systems on adhesion of resin-based root canal sealers. Photomed Laser Surg. 2014;32(3):152–9.
  • Flores DS, Rached FJ Jr, Versiani MA, Guedes DF, Sousa-Neto MD, Pécora JD. Evaluation of physicochemical properties of four root canal sealers. Int Endod J. 2011;44(2):126–35.
  • Torres FFE, Zordan-Bronzel CL, Guerreiro-Tanomaru JM, Chávez-Andrade GM, Pinto JC, Tanomaru-Filho M. Effect of immersion in distilled water or phosphate-buffered saline on the solubility, volumetric change and presence of voids within new calcium silicate-based root canal sealers. Int Endod J. 2020;53(3):385–91.
  • Lin J, Shen Y, Haapasalo M. A comparative study of biofilm removal with hand, rotary nickel-titanium, and self-adjusting file instrumentation using a novel in vitro biofilm model. J Endod. 2013;39(5):658–63.
  • Navabi AA, Khademi AA, Khabiri M, Zarean P, Zarean P. Comparative evaluation of Enterococcus faecalis counts in different tapers of rotary system and irrigation fluids: an ex vivo study. Dent Res J. 2018;15(3):173–9.
  • Plotino G, Cortese T, Grande NM, Leonardi DP, Di Giorgio G, Testarelli L, et al. New technologies to improve root canal disinfection. Braz Dent J. 2016;27(1):3–8.
  • Gursoy H, Ozcakir-Tomruk C, Tanalp J, Yilmaz S. Photodynamic therapy in dentistry: a literature review. Clin Oral Investig. 2013;17(4):1113–25.
  • Chrepa V, Kotsakis GA, Pagonis TC, Hargreaves KM. The effect of photodynamic therapy in root canal disinfection: a systematic review. J Endod. 2014;40(7):891–8.
  • Foote CS. Definition of type I and type II photosensitized oxidation. Photochem Photobiol. 1991;54(5):659.
  • Konopka K, Goslinski T. Photodynamic therapy in dentistry. J Dent Res. 2007;86(8):694–707. Karadas M, Demirbuga S. Influence of a short-time antioxidant application on the dentin bond strength after intracoronal bleaching. Microsc Res Tech. 2019;82(10):1720–7.
  • Harrison MS, Wang Y, Frick KJ, Moniz J, Walker MP. Effects of alpha-tocopherol antioxidant on dentin-composite microtensile bond strength after sodium perborate bleaching. J Endod. 2019;45(8):1053–9.
  • Yavari H, Ghasemi N, Divband B, Rezaei Y, Jabbari G, Payahoo S. The effect of photodynamic therapy and polymer solution containing nano-particles of Ag/ZnO on push-out bond strength of the sealers AH-Plus and MTA Fillapex. J Clin Exp Dent. 2017;9(9):e1109–14.
  • Banci HA, Strazzi-Sahyon HB, Duarte M, Cintra L, Gomes-Filho JE, Chalub LO, et al. Influence of photodynamic therapy on bond strength and adhesive interface morphology of MTA based root canal sealer to different thirds of intraradicular dentin. Photodiagnosis Photodyn Ther. 2020;32:102031.
  • Camacho-Alonso F, Julián-Belmonte E, Chiva-García F, Martínez-Beneyto Y. Bactericidal efficacy of photodynamic therapy and chitosan in root canals experimentally infected with Enterococcus faecalis: an in vitro study. Photomed Laser Surg. 2017;35(4):184–9.
  • Katalinić I, Budimir A, Bošnjak Z, Jakovljević S, Anić I. The photo-activated and photo-thermal effect of the 445/970 nm diode laser on the mixed biofilm inside root canals of human teeth in vitro: a pilot study. Photodiagnosis Photodyn Ther. 2019;26:277–83.
  • Zorita-García M, Alonso-Ezpeleta LÓ, Cobo M, Del Campo R, Rico-Romano C, Mena-Álvarez J, et al. Photodynamic therapy in endodontic root canal treatment significantly increases bacterial clearance, preventing apical periodontitis. Quintessence Int. 2019;50(10):782–9.
  • Neelakantan P, Sharma S, Shemesh H, Wesselink PR. Influence of irrigation sequence on the adhesion of root canal sealers to dentin: a Fourier transform infrared spectroscopy and push-out bond strength analysis. J Endod. 2015;41(7):1108–11.
  • Ramos ATPR, Belizário LG, Venção AC, Jordão-Basso KCF, Rastelli ANS, Andrade MF, et al. Effects of photodynamic therapy on the adhesive interface of fiber posts cementation protocols. J Endod. 2018;44(1):173–8.
  • Marciano MA, Bramante CM, Duarte MA, Delgado RJ, Ordinola-Zapata R, Garcia RB. Evaluation of single root canals filled using the lateral compaction, Tagger’s hybrid, microseal and guttaflow techniques. Braz Dent J. 2010;21(5):411–5.
  • Fickweiler S, Szeimies RM, Bäumler W, Steinbach P, Karrer S, Goetz AE, et al. Indocyanine green: intracellular uptake and phototherapeutic effects in vitro. J Photochem Photobiol B. 1997;38(2–3):178–83.
  • Saha B, Chowdhury S, Sanyal D, Chattopadhyay K, Kumar GS. Comparative study of toluidine blue O and methylene blue binding to lysozyme and their inhibitory effects on protein aggregation. ACS Omega. 2018;3(3):2588–601.
  • Nagai Y, Suzuki A, Katsuragi H, Shinkai K. Effect of antimicrobial photodynamic therapy (aPDT) on the sterilization of infected dentin in vitro. Odontology. 2018;106(2):154–61.
  • Ok E, Ertas H, Saygili G, Gok T. Effect of photoactivated disinfection on bond strength of root canal filling. J Endod. 2013;39(11):1428–30.
  • Tilakchand M, Singh NN, Yeli MM, Naik BD. Evaluation of the antibacterial efficacy of EZLASE diode LASER on the infected root canal system: an in vivo study. J Conserv Dent. 2018;21(3):306–10. Namvar MA, Vahedi M, Abdolsamadi HR, Mirzaei A, Mohammadi Y, Jalilian FA. Effect of photodynamic therapy by 810 and 940 nm diode laser on herpes simplex virus 1: an in vitro study. Photodiagnosis Photodyn Ther. 2019;25:87–91.
  • Aytac Bal F, Ozkocak I, Cadirci BH, Karaarslan ES, Cakdinleyen M, Agaccioglu M. Effects of photodynamic therapy with indocyanine green on Streptococcus mutans biofilm. Photodiagnosis Photodyn Ther. 2019;26:229–34.
  • Sen OG, Kaya M. Effect of root canal disinfection with a diode laser on postoperative pain after endodontic retreatment. Photobiomodul Photomed Laser Surg. 2019;37(2):85–90.
  • Osmari D, Ferreira ACO, Bello MC, Susin AH, Aranha ACC, Marquezan M, et al. Micromorphological evaluation of dentin treated with different desensitizing agents. J Lasers Med Sci. 2013;4(3):140–6.
  • Yilmaz Y, Keles S, Mete A. Temperature changes in the pulpal chamber and the sealing performance of various methods of direct pulp capping of primary teeth. Eur J Paediatr Dent. 2013;14(2):95–100.
  • Kasraei S, Yarmohamadi E, Jahromi PR, Akbarzadeh M. Effect of 940nm diode laser irradiation on microtensile bond strength of an etch and rinse adhesive (Single Bond 2) to dentin. J Dent. 2019;20(1):30–6.
  • Pantoja CAMS, Silva DHD, Soares AJ, Ferraz CCR, Gomes BPFA, Zaia AA, et al. Influence of ethanol on dentin roughness, surface free energy, and interaction between AH Plus and root dentin. Braz Oral Res. 2018;32:e33.
  • Swift EJ Jr. Critical appraisal. Reversal of compromised bonding after bleaching. J Esthet Restor Dent. 2012;24(5):357–61. Titley KC, Torneck CD, Ruse ND, Krmec D. Adhesion of a resin composite to bleached and unbleached human enamel. J Endod. 1993;19(3):112–5.
  • Santos GC, Baia JC, Ribeiro ME, Lima RR, Sousa Júnior MHSE, Loretto SC. Influence of prolonged bleaching with 4% hydrogen peroxide containing calcium and different storage times on the bond strength to enamel. J Contemp Dent Pract. 2019;20(2):216–20.
There are 39 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section ORIGINAL ARTICLE
Authors

İsmail Ozkocak 0000-0003-0820-0069

Hakan Gokturk 0000-0003-3824-2569

İrem Cansu Kara This is me 0000-0003-0583-8999

Project Number -
Publication Date September 27, 2021
Acceptance Date May 31, 2021
Published in Issue Year 2021 Volume: 26 Issue: 3

Cite

Vancouver Ozkocak İ, Gokturk H, Cansu Kara İ. The Effects of Different Photoactive Dyes on the Adhesion of an Epoxy Resin-based Root Canal Sealer. Anatolian Clin. 2021;26(3):225-32.

13151 This Journal licensed under a CC BY-NC (Creative Commons Attribution-NonCommercial 4.0) International License.