Exploring Adaptation Strategies of Common Bean to Phosphorus Deficiency under Field Conditions: Interactions between Nutrient Uptake and Symbiotic Associations

Zohra Touati; Karima Toudert-taleb

doi:10.15832/ankutbd.1794176

Exploring Adaptation Strategies of Common Bean to Phosphorus Deficiency under Field Conditions: Interactions between Nutrient Uptake and Symbiotic Associations

Abstract

Understanding the factors influencing phosphorus (P) and nitrogen (N) use efficiencies under soil P deficiency, is essential for developing strategies to mitigate P limitation. This study was conducted to investigate how common bean copes with P deficiency under Mediterranean agro ecological conditions. Three genotypes (Contender (C), Djadida (Dj), and Tema (T)) were grown across nine field sites in the Tizi-Ouzou province of Algeria. The sites were grouped into three clusters according to soil texture, pH, and altitude. At flowering stage, plants were uprooted and analyzed for growth, nodulation, arbuscular mycorrhizal fungi (AMF) colonization, P and N content, and corresponding nutrient use efficiencies. The study results show highly significant effect of cluster (P<0.001), a significant effect of genotype (P<0.05), and a significant genotype × cluster interaction (P<0.01), on plant growth and P and N Use Efficiencies. Shoot Dry Weight, P and N use efficiencies differ significantly among clusters and genotypes within the same cluster, while AMF colonization didn’t differ between genotypes. These parameters were significantly higher in the clusters 2 and 3 characterized by fine textured and alkaline soils and situated at low altitudes. Nodulation occurred only in the cluster 1 with coarse textured soils, neutral pH and high altitudes. The genotype C performs better in clusters 1 and 2, while T performed better in cluster 3. Interestingly, Dj showed intermediate performances between C and T, across all clusters. Integrating such knowledge into breeding programs could strengthen common bean resilience and productivity in phosphorus-deficient agro-ecosystems.

Keywords

References

Abd-Alla M H, El-Enany A-W E, Nafady N A, Khalaf D M & Morsy F M (2014). Synergistic interaction of Rhizobium leguminosarum bv. Viciae and arbuscular mycorrhizal fungi as a plant growth promoting biofertilizers for faba bean (Vicia faba L.) in alkaline soil. Microbiological Research 169(1): 49‑58. https://doi.org/10.1016/j.micres.2013.07.007
Abenavoli M R, Longo C, Lupini A, Miller A J, Araniti F, Mercati F, Princi M P & Sunseri F (2016). Phenotyping two tomato genotypes with different nitrogen use efficiency. Plant Physiology and Biochemistry 107: 21‑32. https://doi.org/10.1016/j.plaphy.2016.04.021
Adeniji A, Huang J, Li S, Lu X & Guo R (2025). Hot viewpoint on how soil texture, soil nutrient availability, and root exudates interact to shape microbial dynamics and plant health. Plant and Soil 511(1‑2): 69‑90. https://doi.org/10.1007/s11104-024-07020-y
Alkama N (2010). Adaptation de la symbiose rhizobienne chez le haricot à la déficience en phosphore : Détermination de la réponse de la plante en terme d’échanges gazeux et de flux minéraux échangés avec la rhizosphère [These de doctorat, Montpellier, SupAgro]. https://theses.fr/2010NSAM0022
Alliance Bioversity International - CIAT. Importance of bean crop research. Alliance Bioversity International - CIAT. Consulté 4 décembre 2025, à l’adresse https://alliancebioversityciat.org/crops/beans/importance
Basset C, Abou Najm M, Ghezzehei T, Hao X & Daccache A (2023). How does soil structure affect water infiltration? A meta-data systematic review. Soil and Tillage Research 226: 105577. https://doi.org/10.1016/j.still.2022.105577
Bontpart T, Weiss A, Vile D, Gérard F, Lacombe B, Reichheld J-P & Mari S (2024). Growing on calcareous soils and facing climate change. Trends in Plant Science 29(12): 1319‑1330. https://doi.org/10.1016/j.tplants.2024.03.013
Broughton W J, Hernández G, Blair M, Beebe S, Gepts P & Vanderleyden J (2003). Beans (Phaseolus spp.) – model food legumes. Plant and Soil 252(1): 55‑128. https://doi.org/10.1023/A:1024146710611

Brundrett M & Australian Centre for International Agricultural Research (Éds.). (1996). Working with mycorrhizas in forestry and agriculture. Australian Centre for International Agricultural Research.
Chau J F, Bagtzoglou A C & Willig M R (2011). The Effect of Soil Texture on Richness and Diversity of Bacterial Communities. Environmental Forensics 12 (4): 333‑341. https://doi.org/10.1080/15275922.2011.622348
Cresser M S & Parsons J W (1979). Sulphuric—Perchloric acid digestion of plant material for the determination of nitrogen, phosphorus, potassium, calcium and magnesium. Analytica Chimica Acta 109(2): 431‑436. https://doi.org/10.1016/S0003-2670(01)84273-2
Diatta A A, Thomason W E, Abaye O, Thompson T L, Battaglia M L, Vaughan L J, Lo M & Filho J F D C L (2020). Assessment of Nitrogen Fixation by Mungbean Genotypes in Different Soil Textures Using 15N Natural Abundance Method. Journal of Soil Science and Plant Nutrition 20(4): 2230‑2240. https://doi.org/10.1007/s42729-020-00290-2
Diatta A A, Thomason W E, Abaye O, Vaughan L J, Thompson T L, Lo M, Chim B K & Bateman S (2018). Inoculation and Soil Texture Effects on Yield and Yield Components of Mungbean. Journal of Agricultural Science 10(9): 6-16 https://doi.org/10.5539/jas.v10n9p6
Dissanayaka D M S B, Plaxton W C, Lambers H, Siebers M, Marambe B & Wasaki J (2018). Molecular mechanisms underpinning phosphorus use efficiency in rice. Plant, Cell & Environment 41(7): 1483‑1496. https://doi.org/10.1111/pce.13191
Djili K, Daoud Y & Ayache N (1999). Analyse de la distribution varticale et spatiale du calcaire dans les sols de l’Algérie septentrionale. Etude et Gestion des Sols 6(3): 201‑213.
Djouider S I, Gentzbittel L, Jana R, Rickauer M, Ben C & Lazali M (2022). Contribution to Improving the Chickpea (Cicer arietinum L.) Efficiency in Low-Phosphorus Farming Systems : Assessment of the Relationships between the P and N Nutrition, Nodulation Capacity and Productivity Performance in P-Deficient Field Conditions. Agronomy 12(12): 3150. https://doi.org/10.3390/agronomy12123150
Drevon J, Abadie J, Alkama N, Andriamananjara A, Amenc L, Bargaz A, Carlssonn G, Jaillard B, Lazali M, Ghoulam C & Ounane S (2015). Phosphorus Use Efficiency for N2 Fixation in the Rhizobial Symbiosis with Legumes. https://doi.org/10.1002/9781119053095.CH46
Elliott G C & Läuchli A (1985). Phosphorus Efficiency and Phosphate–Iron Interaction in Maize . Agronomy Journal 77(3): 399‑403. https://doi.org/10.2134/agronj1985.00021962007700030011x
Garbaye J (2013). La symbiose mycorhizienne : Une association entre les plantes et les champignons. Quæ. Hemantaranjan A (1988). Iron fertilization In relation to nodulation and nitrogen fixation in French bean ( Phaseolus vulgaris L.). Journal of Plant Nutrition 11(6‑11): 829‑842. https://doi.org/10.1080/01904168809363848
Houassine D, Latati M, Rebouh N Y & Gérard F (2020). Phosphorus acquisition processes in the field : Study of faba bean cultivated on calcareous soils in Algeria. Archives of Agronomy and Soil Science 66(2): 168‑181. https://doi.org/10.1080/03650340.2019.1605166
Hungria M & Vargas M A T (2000). Environmental factors affecting N2 fixation in grain legumes in the tropics, with an emphasis on Brazil. Field Crops Research 65(2‑3): 151‑164. https://doi.org/10.1016/S0378-4290(99)00084-2
Islam M, Siddique K H M, Padhye L P, Pang J, Solaiman Z M, Hou D, Srinivasarao C, Zhang T, Chandana P, Venu N, Prasad J V N S, Srinivas T, Singh R, Kirkham M B & Bolan N (2024). A critical review of soil phosphorus dynamics and biogeochemical processes for unlocking soil phosphorus reserves. Advances in Agronomy 185: 153‑249. https://doi.org/10.1016/bs.agron.2024.02.004
Javot H, Penmetsa R V, Breuillin F, Bhattarai K K, Noar R D, Gomez S K, Zhang Q, Cook D R & Harrison M J (2011). Medicago truncatula mtpt4 mutants reveal a role for nitrogen in the regulation of arbuscule degeneration in arbuscular mycorrhizal symbiosis. The Plant Journal 68(6): 954‑965. https://doi.org/10.1111/j.1365-313X.2011.04746.x
Ketchiemo F T, Fotso B, Djabou A S M, Evina V J E, Essambita J Y, Tang F M E & Niemenak N (2024). Soil Physico-Chemical Properties and Different Altitudes Affect Arbuscular Mycorrhizal Fungi Abundance and Colonization in Cacao Plantations of Cameroon. American Journal of Plant Sciences 15(02): 57‑82. https://doi.org/10.4236/ajps.2024.152005
Kharrat M, Ben Salah H & Hédi Maatougui M (2023). Étude sur la filière des légumineuses alimentaires et fourragères en Afrique du Nord. FAO. https://doi.org/10.4060/cc0460fr
Lawlor D W (2002). Carbon and nitrogen assimilation in relation to yield : Mechanisms are the key to understanding production systems. Journal of Experimental Botany 53(370): 773‑787. https://doi.org/10.1093/jexbot/53.370.773
Lazali M, Brahimi S, Mérabet C, Latati M, Benadis C, Maougal R, Blavet D, Drevon J & Ounane S (2016). Nodular diagnosis of contrasting recombinant inbred lines of Phaseolus vulgaris in multi-local field tests under Mediterranean climate. European Journal of Soil Biology 73: 100‑107. https://doi.org/10.1016/J.EJSOBI.2016.02.002
Makarov M I (2019). The Role of Mycorrhiza in Transformation of Nitrogen Compounds in Soil and Nitrogen Nutrition of Plants : A Review. Eurasian Soil Science 52(2): 193‑205. https://doi.org/10.1134/S1064229319020108
Mathieu C & Pieltain F (2003). Analyse chimique des sols : Méthodes choisies. Éd. Tec & doc. Mukherjee D (2013). Nutrient use efficiency for maximization of crop productivity. Advances in plant physiology 14: 173‑209 .Hemantaranjan scientific Publishers
Nefzaoui A, Ketata H & El M (2012). Agricultural Technological and Institutional Innovations for Enhanced Adaptation to Environmental Change in North Africa. In S. Young (Éd.), International Perspectives on Global Environmental Change. InTech. https://doi.org/10.5772/27175
Niklas K J (2008). Carbon/nitrogen/phosphorus allometric relations across species. The Ecophysiology of Plant-Phosphorus Interactions 7: 9‑30. springer
Ouzounidou G, Skiada V, Papadopoulou K K, Stamatis N, Kavvadias V, Eleftheriadis E & Gaitis F (2015). Effects of soil pH and arbuscular mycorrhiza (AM) inoculation on growth and chemical composition of chia (Salvia hispanica L.) leaves. Brazilian Journal of Botany 38(3): 487‑495. https://doi.org/10.1007/s40415-015-0166-6
Pansu M, Gautheyrou J (2006). Handbook of Soil Analysis : Mineralogical, Organic and Inorganic Methods. Springer Berlin / Heidelberg. Plassard C, Robin A, Le Cadre E, Marsden C, Trap J, Herrmann L, Waithaisong K, Lesueur D, Blanchart E, Lardy L & Hinsinger P (2015). Améliorer la biodisponibilité du phosphore : Comment valoriser les compétences des plantes et les mécanismes biologiques du sol ? https://doi.org/10.17180/S5SS-1X88 in
Püschel D, Janoušková M, Voříšková A, Gryndlerová H, Vosátka M & Jansa J (2017). Arbuscular Mycorrhiza Stimulates Biological Nitrogen Fixation Two Medicago spp. Through Improved Phosphorus Acquisition. Frontiers in Plant Science 8. https://doi.org/10.3389/fpls.2017.00390
Sarkar D, Kar S K, Chattopadhyay A, Shikha Rakshit A, Tripathi V K, Dubey P K & Abhilash P C (2020). Low input sustainable agriculture : A viable climate-smart option for boosting food production in a warming world. Ecological Indicators 115:106412. https://doi.org/10.1016/j.ecolind.2020.106412
Shankar S, Haque E, Ahmed T, Kiran G S, Hassan S & Selvin J (2021). Rhizobia–Legume Symbiosis During Environmental Stress. In N. Shrivastava, S. Mahajan, & A. Varma (Éds.), Symbiotic Soil Microorganisms 60: 201‑220. Springer International Publishing. https://doi.org/10.1007/978-3-030-51916-2_13
Siddiqi M Y & Glass A D M (1981). Utilization index : A modified approach to the estimation and comparison of nutrient utilization efficiency in plants. Journal of Plant Nutrition 4(3): 289‑302. https://doi.org/10.1080/01904168109362919
Somasegaran P & Hoben H J (1994). Handbook for Rhizobia. Springer New York. https://doi.org/10.1007/978-1-4613-8375-8
Sulieman S & Tran L (2015). Phosphorus homeostasis in legume nodules as an adaptive strategy to phosphorus deficiency. Plant science : an international journal of experimental plant biology 239: 36‑43. https://doi.org/10.1016/j.plantsci.2015.06.018
Tajini F, Trabelsi M & Drevon J-J (2012). Combined inoculation with Glomus intraradices and Rhizobium tropici CIAT899 increases phosphorus use efficiency for symbiotic nitrogen fixation in common bean (Phaseolus vulgaris L.). Saudi Journal of Biological Sciences 19(2): 157‑163. https://doi.org/10.1016/j.sjbs.2011.11.003
Van Der Heijden M G A, Martin F M, Selosse M & Sanders I R (2015). Mycorrhizal ecology and evolution : The past, the present, and the future. New Phytologist 205(4): 1406‑1423. https://doi.org/10.1111/nph.13288
Vitousek P M, Menge D N L, Reed S C & Cleveland C C (2013). Biological nitrogen fixation : Rates, patterns and ecological controls in terrestrial ecosystems. Philosophical Transactions of the Royal Society B: Biological Sciences 368(1621): 20130119. https://doi.org/10.1098/rstb.2013.0119
Vitousek P M, Porder S, Houlton B Z & Chadwick O A (2010). Terrestrial phosphorus limitation : Mechanisms, implications, and nitrogen phosphorus interactions. Ecological Applications 20(1): 5‑15. https://doi.org/10.1890/08-0127.1
Xu G, Fan X & Miller A J (2012). Plant Nitrogen Assimilation and Use Efficiency. Annual Review of Plant Biology 63(1): 153‑182. https://doi.org/10.1146/annurev-arplant-042811-105532
Zheng M, Zhu P, Zheng J, Xue L, Zhu Q, Cai X, Cheng S, Zhang Z, Kong F & Zhang J (2021). Effects of soil texture and nitrogen fertilisation on soil bacterial community structure and nitrogen uptake in flue-cured tobacco. Scientific Reports, 11(1): 22643. https://doi.org/10.1038/s41598-021-01957-1

Details

Primary Language

English

Subjects

Land Capability and Soil Productivity, Plant Nutrition and Soil Fertility, Soil Ecology

Journal Section

Research Article

Authors

Zohra Touati ^*
0009-0003-4733-7735
Algeria

Karima Toudert-taleb This is me
0009-0005-0320-576X
Algeria

Publication Date

March 24, 2026

Submission Date

September 30, 2025

Acceptance Date

December 17, 2025

Published in Issue

Year 2026 Volume: 32 Number: 2

DOI

https://doi.org/10.15832/ankutbd.1794176

IZ

https://izlik.org/JA53AH76XP

Cite

RIS / Bibtex

APA

Touati, Z., & Toudert-taleb, K. (2026). Exploring Adaptation Strategies of Common Bean to Phosphorus Deficiency under Field Conditions: Interactions between Nutrient Uptake and Symbiotic Associations. Journal of Agricultural Sciences, 32(2), 396-407. https://doi.org/10.15832/ankutbd.1794176

AMA

1.Touati Z, Toudert-taleb K. Exploring Adaptation Strategies of Common Bean to Phosphorus Deficiency under Field Conditions: Interactions between Nutrient Uptake and Symbiotic Associations. J Agr Sci-Tarim Bili. 2026;32(2):396-407. doi:10.15832/ankutbd.1794176

Chicago

Touati, Zohra, and Karima Toudert-taleb. 2026. “Exploring Adaptation Strategies of Common Bean to Phosphorus Deficiency under Field Conditions: Interactions Between Nutrient Uptake and Symbiotic Associations”. Journal of Agricultural Sciences 32 (2): 396-407. https://doi.org/10.15832/ankutbd.1794176.

EndNote

Touati Z, Toudert-taleb K (March 1, 2026) Exploring Adaptation Strategies of Common Bean to Phosphorus Deficiency under Field Conditions: Interactions between Nutrient Uptake and Symbiotic Associations. Journal of Agricultural Sciences 32 2 396–407.

IEEE

[1]Z. Touati and K. Toudert-taleb, “Exploring Adaptation Strategies of Common Bean to Phosphorus Deficiency under Field Conditions: Interactions between Nutrient Uptake and Symbiotic Associations”, J Agr Sci-Tarim Bili, vol. 32, no. 2, pp. 396–407, Mar. 2026, doi: 10.15832/ankutbd.1794176.

ISNAD

Touati, Zohra - Toudert-taleb, Karima. “Exploring Adaptation Strategies of Common Bean to Phosphorus Deficiency under Field Conditions: Interactions Between Nutrient Uptake and Symbiotic Associations”. Journal of Agricultural Sciences 32/2 (March 1, 2026): 396-407. https://doi.org/10.15832/ankutbd.1794176.

JAMA

1.Touati Z, Toudert-taleb K. Exploring Adaptation Strategies of Common Bean to Phosphorus Deficiency under Field Conditions: Interactions between Nutrient Uptake and Symbiotic Associations. J Agr Sci-Tarim Bili. 2026;32:396–407.

MLA

Touati, Zohra, and Karima Toudert-taleb. “Exploring Adaptation Strategies of Common Bean to Phosphorus Deficiency under Field Conditions: Interactions Between Nutrient Uptake and Symbiotic Associations”. Journal of Agricultural Sciences, vol. 32, no. 2, Mar. 2026, pp. 396-07, doi:10.15832/ankutbd.1794176.

Vancouver

1.Zohra Touati, Karima Toudert-taleb. Exploring Adaptation Strategies of Common Bean to Phosphorus Deficiency under Field Conditions: Interactions between Nutrient Uptake and Symbiotic Associations. J Agr Sci-Tarim Bili. 2026 Mar. 1;32(2):396-407. doi:10.15832/ankutbd.1794176