Activation of Synergistic Plant-Soil-Biofertilizers Responses by Calcium and Phosphate: Mitigation of Salinity Stress

Hamida Sadji; Assia Chaker-Haddadj; Fahima Nabi; Farida Taguett; Fatiha Aid

doi:10.15832/ankutbd.1894208

Activation of Synergistic Plant-Soil-Biofertilizers Responses by Calcium and Phosphate: Mitigation of Salinity Stress

Abstract

As salt-sensitive crops, the performance of legumes in resisting salinity should be improved through accessible, less expensive and commonly practiced alternatives. Our study investigated the potential benefits of coupling the activation of endogenous phosphate-solubilizing bacteria with calcium phosphate amendment, focusing on saline soil fertility, the activation of the plants' antioxidant defense mechanisms, and the phosphate solubilization efficiency of the PSBs in plants growing under salinity conditions. There were four factors in the experimental treatments; i) control (C), ii) two levels of salinity (NaCl) 0.3% and 0.9%, iii) two levels of calcium-phosphate (Ca-P): (21% Ca x 41%P), (48% Ca x 92% P) and iii) combined application (NaCl x Ca P), applied at the seedling stage. Salinity significantly reduced water uptake, physiological parameters and affected root growth strategy (-60%). PCA confirmed that stressed plants showed a positive association between antioxidant parameters which could related to overproduction of ROS, decreased phosphate solubilizing activity, destroyed soil and reduced plant tolerance to salt stress. Application of combined (NaCl x Ca-P) significantly improved the antioxidant response with accumulation of proteins and proline, increase in water uptake, chlorophyll pigments, maintain cell turgor, root hair density (70%), root hair length (71%), complete histological root formation, improved the physicochemical quality of the soil where EC decreased by about 20 % and pH became neutral, and increased the PSB number (80%). The use of Ca-P with salinity could reestablish physiological homeostasis, complete anatomy, and better plant growth. It also enhanced the physicochemical and biological properties of the saline soil. Interactivity in the plant-soil-rhizobacteria system could be a viable option to positively influence plant resilience against salt stress and saline soil rehabilitation.

Keywords

References

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Details

Primary Language

English

Subjects

Plant Physiology, Plant Nutrition and Soil Fertility

Journal Section

Research Article

Authors

Hamida Sadji ^*
0009-0000-3329-9019
Algeria

Assia Chaker-Haddadj This is me
0009-0005-0113-4988
Algeria

Fahima Nabi This is me
0000-0002-9046-6546
Algeria

Farida Taguett This is me
0000-0002-2793-057X
Algeria

Fatiha Aid
0000-0002-7614-4356
Algeria

Publication Date

October 12, 2026

Submission Date

February 20, 2026

Acceptance Date

June 11, 2026

Published in Issue

Year 2026 Volume: 32 Number: 4

DOI

https://doi.org/10.15832/ankutbd.1894208

IZ

https://izlik.org/JA95LG44UC

Cite

RIS / Bibtex

APA

Sadji, H., Chaker-Haddadj, A., Nabi, F., Taguett, F., & Aid, F. (2026). Activation of Synergistic Plant-Soil-Biofertilizers Responses by Calcium and Phosphate: Mitigation of Salinity Stress. Journal of Agricultural Sciences, 32(4), 1132-1148. https://doi.org/10.15832/ankutbd.1894208

AMA

1.Sadji H, Chaker-Haddadj A, Nabi F, Taguett F, Aid F. Activation of Synergistic Plant-Soil-Biofertilizers Responses by Calcium and Phosphate: Mitigation of Salinity Stress. J Agr Sci-Tarim Bili. 2026;32(4):1132-1148. doi:10.15832/ankutbd.1894208

Chicago

Sadji, Hamida, Assia Chaker-Haddadj, Fahima Nabi, Farida Taguett, and Fatiha Aid. 2026. “Activation of Synergistic Plant-Soil-Biofertilizers Responses by Calcium and Phosphate: Mitigation of Salinity Stress”. Journal of Agricultural Sciences 32 (4): 1132-48. https://doi.org/10.15832/ankutbd.1894208.

EndNote

Sadji H, Chaker-Haddadj A, Nabi F, Taguett F, Aid F (October 1, 2026) Activation of Synergistic Plant-Soil-Biofertilizers Responses by Calcium and Phosphate: Mitigation of Salinity Stress. Journal of Agricultural Sciences 32 4 1132–1148.

IEEE

[1]H. Sadji, A. Chaker-Haddadj, F. Nabi, F. Taguett, and F. Aid, “Activation of Synergistic Plant-Soil-Biofertilizers Responses by Calcium and Phosphate: Mitigation of Salinity Stress”, J Agr Sci-Tarim Bili, vol. 32, no. 4, pp. 1132–1148, Oct. 2026, doi: 10.15832/ankutbd.1894208.

ISNAD

Sadji, Hamida - Chaker-Haddadj, Assia - Nabi, Fahima - Taguett, Farida - Aid, Fatiha. “Activation of Synergistic Plant-Soil-Biofertilizers Responses by Calcium and Phosphate: Mitigation of Salinity Stress”. Journal of Agricultural Sciences 32/4 (October 1, 2026): 1132-1148. https://doi.org/10.15832/ankutbd.1894208.

JAMA

1.Sadji H, Chaker-Haddadj A, Nabi F, Taguett F, Aid F. Activation of Synergistic Plant-Soil-Biofertilizers Responses by Calcium and Phosphate: Mitigation of Salinity Stress. J Agr Sci-Tarim Bili. 2026;32:1132–1148.

MLA

Sadji, Hamida, et al. “Activation of Synergistic Plant-Soil-Biofertilizers Responses by Calcium and Phosphate: Mitigation of Salinity Stress”. Journal of Agricultural Sciences, vol. 32, no. 4, Oct. 2026, pp. 1132-48, doi:10.15832/ankutbd.1894208.

Vancouver

1.Hamida Sadji, Assia Chaker-Haddadj, Fahima Nabi, Farida Taguett, Fatiha Aid. Activation of Synergistic Plant-Soil-Biofertilizers Responses by Calcium and Phosphate: Mitigation of Salinity Stress. J Agr Sci-Tarim Bili. 2026 Oct. 1;32(4):1132-48. doi:10.15832/ankutbd.1894208