Physiological Responses of Gamma-Irradiated Onion Bulbs during Storage

Abstract

The present study aimed to evaluate the effects of different doses of gamma radiation on some physiological characters of onion genotypes and the expression of PAL gene in the best interaction of gamma irradiation dose and onion genotype treatment. To this aim, four onion genotypes (White-Qom, White-Neyshabour, Red-Ridge-Lump, and Red-Ray-Corrugated) irradiated at 0, 30, 60, 90, 120, and 150 Gy. After four months of storage (at 10-15 °C and 70% relative humidity), the effects of gamma rays on the dry matter (DM), protein content, phenylalanine ammonia lyase (PAL) and peroxidase (POD) were investigated. In addition, the expression of PAL gene in the best interaction of gamma irradiation dose and onion genotype treatment was assessed. The result indicated that POD activity was increased by most of the gamma irradiation levels; however, the protein content and PAL activity were decreased. Moreover, dry matter content was found to be highly genotype-dependent. A linear regression (R2= 0.82) between PAL activity and gamma irradiation levels, was observed. PAL activity decreased with increasing in gamma irradiation level, while the expression rate of PAL gene was not significantly changed between irradiated and non-irradiated control, indicating that the radiation might not have direct effects on the gene regulatory elements. These results suggest that gamma irradiation could reduce the PAL activity possibly by controlling abiotic stress sources such as fungal and bacterial stresses.

Keywords

• Gamma radiation
• Allium cepa
• PAL activity
• Gene expression

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