Relationship Between Resistance Against Neonicotinoids and Esterase Enzyme for Myzus persicae (Sulzer) (Hemiptera:Aphididae) Populations in South of Turkey

Abstract

In this study, the development of imidacloprid and acetamiprid resistance in Myzus persicae populations and the relationship between neonicotinoid resistance and esterase enzyme were investigated in seven Myzus persicae populations which is collected from greenhouse pepper production areas in 2018. In order to determine the resistance ratios of aphid populations against the insecticides, 1 control and 6 doses were used. Each insecticide dose was used in 3 replicates and 25 adult female individuals were used in each replication. For imidacloprid, the highest and the lowest resistance ratios were found to be 6.88 and 3.19-fold, in K-4 and D populations, respectively. For acetamiprid, the highest and the lowest resistance ratios were found to be 7.35 and 2.72-fold, in K-1 and E-2 populations, respectively. Also, highest and lowest esterase activities were found to be 2.60 and 1.75 mOD min-1 mg-1 in K-4 and E-2 populations, respectively. According to the results of this study, imidacloprid and acetamiprid resistance determined in some Myzus persicae populations may be related to esterase enzyme. However, detailed studies are required to establish a clear relationship between resistance and enzyme.

Keywords

• Acetamiprid
• Esterase
• Imidacloprid
• Myzus persicae

References

1. Afzal M B S, Abbas N & Shad S A (2015). Inheritance realized heritability and biochemical mechanism of acetamiprid resistance in the cotton mealybug, Phenacoccus solenopsis Tinsley (Homoptera:Pseudococcidae). Pesticide Biochemistry and Physiology 122: 44–49
2. Barber M D, Moores G D, Tatchell G M, Vice W E & Denholm I (1999). Insecticide resistance in the currant-lettuce aphid, Nasonovia ribisnigri (Hemiptera: Aphididae) in the UK. Bulletien Entomoligal Reserch 89: 17-23
3. Bass C & Field L M (2011). Gene amplification and insecticide resistance. Pest Management Science 67(8): 886-890
4. Bass C, Puinean M, Zimmer C T, Denholm I, Field L M & Foster S P (2014). The evolution of insecticide resistance in the peach-potato aphid,Myzus persicae. Insect Biochemical Molecular Biology 51:41–51
5. Blackman R L & Eastop V F (2007). Aphids as Crop Pests. Wallingford, CABI
6. Cassanelli S, Cerchiari B, Giannini S, Bizzaro D, Mazzoni E & Manicardi G C (2005). Use of the RFLP-PCR diagnostic test for characterizing MACE and kdr insecticide resistance in thepeach potato aphid Myzus persicae. Pest Management Science 61(1): 91-6
7. Charaabi K, Boukhris‐Bouhachem S, Makni M, Fenton B & Denholm I (2016). Genetic variation in target‐site resistance to pyrethroids and pirimicarb in Tunisian populations of the peach potato aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). Pest Management Science 72(12): 2313-2320
8. Charaabi K, Boukhris‐Bouhachem S, Makni M & Denholm I (2018). Occurrence of target‐site resistance to neonicotinoids in the aphid Myzus persicae in Tunisia, and its status on different host plants. Pest Management Science 74(6): 1297-1301

9. Criniti A, Mazzoni E, Cassanelli S, Cravedi P, Tondelli A, Bizzaro D & Manicardi G C (2008). Biochemical and molecular diagnosis of insecticide resistance conferred by esterase, MACE, kdr and super-kdr based mechanisms in Italian strains of the peach potato aphid, Myzus persicae (Sulzer). Pesticide Biochemistry and Physiology 90(3):168-174
10. Denholm I & Jespersen J B (1998). Insecticide resistance management in Europe: recent developments and prospects. Pesticide Science 52: 193-195
11. Devonshire A L (1989). The role of electrophoresis in the biochemical detection of insecticide resistance. In: Electrophoretic Studies on Agricultural Pests. Clarendon Press, Oxford
12. Devonshire A L, Devine G J & Moores G D (1992). Comparison of microplate esterase assays and immunoassay for identifying insecticide resistant variants of Myzus persicae (Homoptera: Aphididae). Bulletin Entomoloigal Resarch 82: 459-463
13. Devonshire A L & G D Moores (1982). A carboxylesterase with broad substratespecifıcity causes organophosphorus, carbamate and pyrethroid resistance inpeach-potato aphids (Myzus persicae). Pesticide Biochemistry and Physiology 18: 235-246
14. de Little S, Edwards O, R van Rooyen A, Weeksa A & Uminaa P A (2017). Discovery of metabolic resistance to neonicotinoids in green peach aphids (Myzus persicae) in Australia. Pest Managament Science 73: 1611–1617
15. Eleftherianos I, Foster S P, Williamson M S & Denholm I (2008).Characterization of the M918T sodium channel gene mutation associated with strong resistance to pyrethroid insecticides in the peach-potato aphid, Myzus persicae (Sulzer). Bulletin Entomoloigal Resarch 98:183–191
16. Foster S P, Cox D, Oliphant L, Mitchinson S & Denholm I (2008). Correlated responses to neonicotinoid insecticides in clones of the peach-potatoaphid, Myzus persicae (Hemiptera: Aphididae). Pest Managament Science 64: 1111-1114
17. Gao X W, Zheng B Z & Cao B J (1992). Reisitance in Myzus persicae to organophosohoru sand carbamate insecticides in China. Journal of Plant Protection 19: 365–371
18. LeOra Software (1994). “Polo-pc: a user’s guide to probit or logit analysis leora software”, Berkeley, 28
19. Moores G D (1995). New resistant-aphid threat from abroad. Arable Farming 22(7):10-13
20. Moores G D, Gao X, Denholm I & Devonshire A L (1996). Characterization of insensitive acetylcholinesterase in insecticide-resistant cotton aphids, Aphis gossypii Glover (Homoptera: Aphididae). Pesticide Biochemistry and Physiology 56: 102-110
21. Ornstein L & Davis B J (1964). Disc electrophoresis I. Background and theory. Annals of the New York Academy of Sciences 121: 321-349
22. Panini M, Dradi D, Marani G, Butturini A & Mazzoni E (2014). Detecting the presence of target-site resistance to neonicotinoids and pyrethroids in Italian populations of Myzus persicae. Pest Managament Science 70:931–938
23. Puinean A M, Foster S P, Oliphant L, Denholm I, Field L M, Millar N S, Williamso M. S & Bass C (2010). Amplification of a cytochrome P450 gene is associated with resistance to neonicotinoid insecticides in the aphid Myzus persicae. PLoS https://doi.org/10.1371/journal.pgen.1000999
24. Sparks T C & Nauen R (2015). IRAC: Mode of action classification and insecticide resistance management. Pesticide Biochemistry and Physiology 121:122-128
25. Song S S, Oh H K & Motoyama N (1995). Insecticide resistance mechanism in the spiraea aphid, Aphis citricola (van der Goot). Korean Journal Applied Entomology 34: 89-94
26. Van Emden H F & Harrington R (2007). Aphids as Crop Pests. CABI Publishing, Wallingford, UK
27. Voudouris C C,Williamson M S, Skouras P J, Kati A N, Sahinoglou A J & Margaritopoulos J T (2017). Evolution of imidacloprid resistance in Myzus persicae in Greece and susceptibility data for spirotetramat. Pest Management Science 73(9): 1804-1812
28. Wang K Y, Liu T X, Yu C H, Jıang X Y & Yi M Q (2002). Resistance of Aphis gossypii (Homoptera: Aphididae) to fenvalerate and imidacloprid and activities of detoxification enzymes on cotton and cucumber. Journal of Economic Entomology 95(2): 407-413