Fluoresan Pseudomonas spp. ile Pamuk Fide Kök Çürüklüğü Hastalıklarının Biyolojik Mücadelesi

Abstract

Fide kök çürüklüğü pamuk dahil pek çok bitkide görülen ve ekonomik kayıplara yol açan önemli bir hastalıktır. Fungisitler hastalığa karşı mücadelede kullanılması sonucu, çevre ve insan sağlığı olumsuz yönde etkilenmektedir. Çalışmada, fluoresan pseudomonas FP bakterilerinin fide kök çürüklüğü hastalık etmenlerine karşı in-vitro ve in-vivo koşullarda etkilerinin belirlenmesi amaçlanmıştır. Pamuk ve tarladaki yabancı otların rizosferinden izole edilen 59 adet FP izolatı ile in vitro’da ikili kültür testleri yürütülmüştür. Daha sonra etkili bulunan FP izolatları tohuma uygulanarak fide kök çürüklüğü etmenlerine karşı antagonistik etkileri iklim odasında araştırılmıştır. İkili kültür testlerinde, Rhizoctonia solani’ye karşı en yüksek etkiyi FP40 % 49.60 ; Fusarium sp.’ye karşı en yüksek etkiyi FP51 % 43.80 , FP48 % 43.50 ve FP35 % 43.10 ; Pythium deliense’ye karşı en yüksek etkiyi FP57 % 59.80 , FP52 % 57.80 ve FP56 % 57.60 izolatları göstermiştir. İklim odasında, FP35 ve FP57 izolatları her üç patojene karşı % 70’in üzerinde koruma sağlarken, ticari fungisitler Vitavax, Maxim ve biyofungisit Subtilex kadar etkili bulunmuş ve ümitvar sonuçlar elde edilmiştir.

Keywords

• Biyolojik mücadele
• Biyofungisit
• Fide hastalığı
• Fluoresan pseudomonas

Biological Control of Cotton Seedling Diseases by Fluorescent Pseudomonas spp

Abstract

Seedling root rot seen in many plants including cotton is an important disease that leads to large economic losses. Human health and the environment are negatively affected as a result of using fungicides for disease control. The goal of this study was to determine the effects of fluorescent Pseudomonas FP bacteria against seedling root rot pathogens both in vitro and in vivo conditions. 59 FP isolates obtained from the rhizosphere of cotton and weeds on the field were tested by dual-culture assays in vitro. After applying effective FP isolates on the seeds, antagonistic effects against the seedling root rot pathogens were investigated in a climate chamber. Resulting of dual-culture tests, FP40 had maximum effect 49.60% against Rhizoctonia solani. Besides, FP51, FP48 and FP35 had highest impact as 43.80%, 43.50%, and 43.10% against Fusarium sp., respectively. Pythium deliense was mostly effected by FP57 59.80% , FP52 57.80% and FP56 57.60% . While isolates FP35 and FP57 provided protection over 70% against all three pathogens in a climate chamber, they were as effective as commercial fungicides Vitavax and Maxim and biofungicide Subtilex and shown promising results

Keywords

• Biocontrol
• Biofungicide
• Seedling disease
• Fluorescent pseudomonas

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