Effect of Heat Shock Treatment on Microspore Embryogenesis in Brassica oleracea Species

Abstract

Heat shock treatments are widely used to induce microspore embryogenesis in Brassica species. In this study, the effect of high temperature treatment 32 °C and 35 °C for 2 days on microspore embryogenesis was investigated in six genotypes of Turkish white head cabbage Yalova-1, Ercis, 177 C, 177 T, 531 C, 538 C , three genotypes of Turkish kale Balkaya, Yanmaz, Karadere 077 and five commercial F1 ornamental kale hybrids Red Piegon, Victoria Piegon, Red Chidori, white Kamome, and Pink Kamome . Microspore-derived embryos formation differed depending on genotype and high temperature. The highest embryo yield was obtained as 9.92 embryo per petri dish in cv. Yalova-1, 11.13 embryo per petri dish in Pink Kamome F1 at 32 °C, and 5.63 embryo per petri dish in cv. Karadere 077 at 35 °C

Keywords

• Embryo
• Genotype
• High temperature
• Microspore culture
• White cabbage
• Kale

Sıcaklık şoku uygulamaları Brassica türlerinde mikrospor embriyogenesisi uyarmak amacıyla yaygın olarak kullanılmaktadır

Abstract

. Bu çalışmada, 6 Türk beyaz baş lahana Yalova-1, Ercis, 177 C, 177 T, 531 C, 538 C , 3 Türk yaprak lahana Karadere 077, Balkaya, Yanmaz çeşit ve genotipi ile 5 ticari F1 hibrid süs lahanası çeşidinde Red Piegon, Victoria Piegon, Red Chidori, White Kamome, and Pink Kamome yüksek sıcaklık uygulamalarının 32 °C ve 35 °C, 2 gün mikrospor embriyogenesisi üzerine etkisi araştırılmıştır. Mikrospor kökenli embriyo oluşumu genotip ve yüksek sıcaklığa göre farklılık göstermiştir. En yüksek embriyo oluşumu 32 °C’de 9.92 embriyo petri-1 değeri ile Yalova-1 çeşidinden, 11.13 embriyo petri-1 değeri ile Pink Kamome F1 çeşidinden ve 35 °C’de ise 5.63 embriyo petri-1 Karadere 077 çeşidinden elde edilmiştir

Keywords

• Embriyo
• Genotip
• Yüksek sıcaklık
• Mikrospor kültürü
• Beyaz baş lahana
• Yaprak lahana

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