The Sensitivity Against Some Acaricides and the Detoxification Enzyme Levels of Panonychus ulmi Koch Collected from Apple Orchards in Isparta

Abstract

Susceptibility of Panonychus ulmi Koch populations collected from apple orchards in Isparta province was studied against etoxazole, spirodiclofen and hexythiazox, and their detoxification enzyme esterase, glutathione-S-transferase GST , monooxygenaz cytochrome P450 P450 and acetylcholinesterase AChE levels were determined. For this purpose, 13 P. ulmi populations were collected from Isparta province and its districts during summer periods of 2012- 2013. Acaricides were applied to mites by spray tower-leaf bioassay method. The data were analyzed by the program POLO PC and LC50 values were calculated. The resistance ratios are calculated by comparing LC50 values of field populations with LC50 values of the susceptible population. The resistance ratios in P. ulmi populations ranged from

Keywords

• Panonychus ulmi,Resistance,Esterase,GST,P450,AChE

Isparta İli Elma Bahçelerinden Toplanan Panonychus ulmi Koch’nin Bazı Akarisitlere Karşı Duyarlılık ve Detoksifikasyon Enzim Düzeyleri

Abstract

Isparta ili elma bahçelerinden toplanan Panonychus ulmi Koch popülasyonlarının etoxazole, spirodiclofen, hexythiazox’a karşı duyarlılık ve detoksifikasyon esteraz, glutation-S-transferaz GST , sitokrom P450 monooxygenaz P450 ve asetilkolinesteraz AChE enzim düzeyleri belirlenmiştir. Bu amaçla Isparta ili ve ilçelerinden 2012 ve 2013 yılı üretim sezonunda toplam 13 adet P. ulmi popülasyonu toplanmıştır. Akarisiter akarlara ilaçlama kulesi-yaprak biyoassay yöntemi ile uygulanmıştır. Elde edilen veriler POLO PC programı ile analiz edilmiş ve LC50 değerleri elde edilmiştir. Tarla popülasyonlarının LC50 değerleri hassas popülasyonun LC50 değerlerine oranlanarak direnç oranları hesaplanmıştır. P. ulmi popülasyonlarının spirodiclofen’e karşı direnç düzeyleri

Keywords

• Panonychus ulmi,Direnç,Esteraz,GST,P450,AChE

References

1. Auger P, Bonafos R, Guichou S & Kreiter S (2003). Resistance to fenazaquin and tebufenpyrad in Panonychus ulmi Koch (Acari: Tetranychidae) populations from South of France apple orchards. Crop Protection 22: 1039-1044
2. Bradford M M (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254
3. Chauret N, Gauthier A & Nicoll-Griffith D A (1998). Effect of common organic solvents on in vitro cytochrome P450-mediated metabolic activities in human liver microsomes. Drug Metabolism and Disposition 26: 1-4
4. Croft B A (1975). Integrated Control of Apple Mites. Michigan State University Extension Service Bulletin E-825
5. Demircan V & Yılmaz H (2005). Isparta ili elma üretiminde tarımsal ilaç kullanımının çevresel duyarlılık ve ekonomik açıdan analizi. Ekoloji 14(57): 15-25
6. GTHB (2011). Elma Entegre Mücadele Teknik Talimatı. Gıda, Tarım ve Hayvancılık Bakanlığı, Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü Bitki Sağlığı Araştırmaları Daire Başkanlığı 188, Ankara
7. Hamingway J (1998). Techniques to Detect Insecticide Resistance Mechanisms (Field and Laboratory Manual). World Health Organization Department of Disease Prevention and Control WHO Communicable Diseases U.S.A
8. Kramer T & Nauen R (2011). Monitoring of spirodiclofen susceptibility in field populations of European red mites, Panonychus ulmi (Koch) (Acari: Tetranychidae), and the cross-resistance pattern of a laboratory selected strain. Pest Management Science 67: 1285-1293

9. Kumral N A & Kovancı B (2007). Susceptibility of female populations of Panonychus ulmi (Koch) (Acari:Tetranyhidae) to some acaricides in apple orchards. Journal of Pest Science 80: 131-137
10. Kumral N A, Susurluk H, Gençer N S & Gürkan M O (2009). Resistance to chlorpyrifos and lambda- cyhalothrin along with detoxifying enzyme activities in field-collected female populations of European red mite. Phytoparasitica 37: 7-15
11. Leora Software (1994). Polo-pc: a User’s Guide to Probit or Logit Analysis Leora Software, 28 pp., Berkeley, CA
12. Pree D J, Whitty K J & Driel L V (2005). Baseline susceptibility and cross resistances of some new acaricides in the European red mite, Panonychus ulmi. Experimental and Applied Acarology 37: 165-171
13. Rauch N & Nauen R (2002). Spirodiclofen resistance risk assessment in Tetranychus urticae (Acari: Tetranychidae): A biochemical approach. Pesticide Biochemistry and Physiology 74(2): 91-101
14. Rose R L, Barbhaiya R, Roe G, Rock E & Hodgson E (1995). Cytochrome p-450 associated ınsecticide resistance and the development of biochemical diagnostic assays in Heliothis virescens. Pesticide Biochemistry and Physiology 51: 178-191
15. SAS (1999). Statistical Analysis Systems User’s Guide, 8th Edn. SAS Institute INC, Raleigh
16. Stumpf N, Zebitz C P W, Kraus W, Moores G D & Nauen R (2001). Resistance to organophosphates and biochemical genotyping of acetylcholinesterases in Tetranychus urticae (Acari: Tetranychidae). Pesticide Biochemistry and Physiology 69: 131-142
17. Stumpf N & Nauen R (2002). Biochemical markers linked to abamectin resistance in Tetranychus urticae (Acari: Tetranychidae). Pesticide Biochemistry and Physiology 72: 111-121
18. Thwahite W G (1991). Resistance to clofentezine and hexythiazox in Panonychus ulmi from apples in Australia. Experimental and Applied Acarology 11: 73-80
19. TÜİK (2014). Türkiye İstatistik Kurumu. http://www. tuik.gov.tr (Erişim tarihi: 6.5.2014)
20. Van Pottelberge S, Van Leeuwen T, Khajehali J & Tirry L (2009). Genetic and biochemical analysis of a laboratory-selected spirodiclofen-resistant strain of Tetranychus urticae Koch (Acari: Tetranychidae). Pest Management Science 65(4): 358-366
21. Yorulmaz-Salman S & Ay R (2012). Isparta ili elma bahçelerinden toplanan avcı akar Neoseiulus californicus (Acari: Phytoseiidae) popülasyonlarının bazı akarisitlere karşı direnç düzeyleri ve direnç mekanizmaları. SDÜ Fen Bilimleri Enstitüsü Dergisi 16(2): 122-132
22. Yorulmaz-Salman S & Ay R (2013). Avcı akar Neoseiulus californicus (Acari: Phytoseiidae) popülasyonlarının üç farklı akarisite karşı duyarlılık ve detoksifikasyon enzim düzeylerinin belirlenmesi. Türkiye Entomoloji Dergisi 37(1): 105-116
23. Yorulmaz-Salman S, Aydınlı F & Ay R (2014). Predator akar Neoseiulus californicus (McGregor) (Acari: Phytoseiidae)’un dört farklı popülâsyonunun spirodiclofen, hexythiazox, etoxazole karşı direnç düzeyleri ve direnç mekanizmalarının belirlenmesi. Türkiye Biyolojik Mücadele Dergisi 5(1): 81-98
24. Yu D Y, Wang C F, Yu Y, Huang Y Q, Yao J A & Hu J F (2011). Laboratory selection for spirodiclofen resistance and cross-resistance in Panonychus citri. African Journal of Biotechnology 10: 3424-3429