Evaluation of Fire Blight Erwinia amylovora Disease Reaction of Pear Hybrid Combinations

Abstract

Fire blight caused by pathogenic bacterium Erwinia amylovora, is the serious disease of pear. Since there is no effective chemical management to this disease except antibiotic-type compounds, it is very important to improve new fire blight resistant cultivars. In this research, it was aimed to select and develop fire blight resistant pear types and to determine fire blight susceptibility levels of pear hybrids, obtained from different projects. Hybrid plants were inoculated by shoot injections twice each year. Evaluations were made through necrotic shoot rate and susceptibility levels of hybrids were determined. During the experiments, 7036 hybrid pear seedlings inoculated, and 12.28% of them were found as “very low susceptibility” A , 3.62% as “low susceptibility” B classes

Keywords

• Artificial inoculation,Erwinia amylovora,Hybrid,Pear

Farklı Armut Melezleme Kombinasyonlarında Ateş Yanıklığı Hastalığı Erwinia amylovora Reaksiyonunun Değerlendirilmesi

Abstract

Erwinia amylovora bakterisinin neden olduğu ateş yanıklığı hastalığı, armudun önemli hastalıklarından biridir. Hastalığa karşı, antibiyotikler dışında etkili bir kimyasal mücadele bulunmaması nedeni ile ateş yanıklığına dayanıklı yeni çeşitlerin geliştirilmesi çok önemlidir. Bu araştırmada, ateş yanıklığına dayanıklı armut tiplerinin seçilmesi ve geliştirilmesinin hedeflenmesinin yanısıra farklı projelerden farklı melezleme kombinasyonlarıyla elde edilen melezlerin ateş yanıklığına hassasiyet seviyelerinin belirlenmesi amaçlanmıştır. Melez bitkiler sürgün enjeksiyonu yoluyla her yıl iki kez inokule edilmişlerdir. Nekroz oluşturan sürgün oranına göre değerlendirmeler gerçekleştirilmiş ve hassasiyet sınıfları belirlenmiştir. Denemeler boyunca, inokule edilen toplam 7036 melez bitkiden, % 12.28’i “çok az duyarlı” A , % 3.62’si ise “az duyarlı” B sınıfta yer almışlardır.

Keywords

• Suni inokulasyon,Erwinia amylovora,Melez,Armut

References

1. Aldwinckle H S, Borejsza-Wysocka E E, Malnoy M, Brown S K, Norelli J L, Beer S V, Meng X, He S Y & Jin Q L (2003). Development of fire blight resistant apple cultivars by genetic engineering. Acta Horticulturae 622: 105-111
2. Aysan Y, Tokgönül S, Çinar Ö & Küden A (1999). Biological, chemical, cultural control methods and determination resistant cultivars to fire blight in pear orchards in the Eastern Mediterranean Region of Turkey. Acta Horticulturae 489: 549-553
3. Aysan Y, Şahin F, Saygılı H, Mirik M & Kotan R (2004). Phenotypic characterization of Erwinia amylovora from pome fruits in Turkey. Acta Horticulturae 459-463
4. Bell A C, Ranney T G, Eaker T A & Sutton T B (2005). Resistance to fire blight among flowering pears and quince. Hortscience 40: 413-415
5. Bergamaschi M, Rivalta L, Sirri S, Biondi E, Ramili F & Bazzi C (2006). Reactivity to fire blight of new promising pear selections. Acta Horticulturae 704: 571-577
6. Brown S K (2003). Pome fruit breeding: Progress and prospects. Acta Horticulturae 622: 19-34
7. Chevreau E, Malnoy M, Mourgues F, Brisset M N & Reynoird J P (2000). Genetic engineering of pear for increased fire blight resistance. Acta Horticulturae 538: 639-643
8. Çıtır A & Mirik M (1999). Fire blight of pome fruits and search for resistant or tolerant cultivars in Amasya and Tokat Regions in Turkey. Acta Horticulturae 489: 215-220

9. Durel C E, Guérif P, Belouin A & Le Lezec M (2004). Estimation of fire blight resistance heritability in the French pear breeding program using a pedigree-based approach. Acta Horticulturae 663: 251-256
10. Ellis M A (2010). Fireblight of Apples and Pears. The Ohio State University Extention, Agricultural and Natural Resources Fact Sheet. HYG-302-08. http:// ohioline.osu.edu/hyg-fact/3000/pdf/HYG_3002_08. pdf (Erişim tarihi: 15.09.2014)
11. Evrenosoğlu Y, Mısırlı A, Saygılı H, Bilen E, Boztepe Ö & Acarsoy N (2011). Evaluation of susceptibility of different pear hybrid populations to fire blight (Erwinia amylovora). Notulae Botanicae Horti Agrobotanici Cluj-Napoca 39(1): 226-237
12. Evrenosoğlu Y, Mısırlı A, Aysan Y, Saygılı H, Boztepe Ö, Horuz S, Acarsoy N, Bilen E, Baykul A & Yazıcı İ (2014). Detection of reaction of F1 hybrid pear population against fire blight disease caused by Erwinia amylovora. Ege Üniversitesi Ziraat Fakültesi Dergisi 51(2): 185-191
13. Hasler T & Kellerhals M (1997). Feuerbrandanfalligkeit Verschiedener Apfelund Birnensorten. Research Institute of Pomology and Floriculture, Skierniewice- Poland
14. Hevesi M, Göndör M, Kása K, Honty K & Tóth M G (2004). Traditional and commercial apple and pear cultivars as sources of resistance to fireblight. EPPO/ OEPP Bulletin 34(3): 377-380
15. Honty K, Göndör M, Toth M, Kasa K & Hevesi M (2006). Susceptibility of pear cultivars to fire blight in Hungary. Acta Horticulturae 704: 583-587
16. Hunter D M & Layne R E C (2004). Recent pear and apricot introductions from the AAFC-Harrow tree fruit breeding programs. Acta Horticulturae 663: 907-910
17. Layne E C & Quamme H A (1975). Pears. J Janick & J N Moore (Eds), Advances in Fruit Breeding, Purdue University Press, West Lafayette, Indiana, pp. 38-70
18. McGraw D (2006). Pollination requirements for fruits and nuts, Oklahama Cooperative Extension Fact Sheets, F-6229. http://pods.dasnr.okstate.edu/docushare/dsweb (Erişim tarihi: 01.03.2015)
19. Momol M T, Yeğen O, Basım H & Rudolph K (1992). Identification of Erwinia amylovora and the occurrence of fire blight of pear in Western Mediterranean Region of Turkey. Journal of Turkish Phytopathology 21(1): 41-47
20. Öden S (1999). Occurrence of fire blight in pear trees grown in Van and around. Acta Horticulturae 489: 107-111
21. Öztürk G, Basım E, Basım H, Emre R A, Karamürsel Ö F, Eren İ, İşçi M & Kaçal E (2011). Kontrollü melezleme yoluyla ateş yanıklığı (Erwinia amylovora) hastalığına karşı dayanıklı yeni armut çeşitlerinin geliştirilmesi: İlk meyve gözlemleri. VI. Horticultural Congress, 04- 08 November, Şanlıurfa, Turkey, Cilt 1: Meyvecilik, pp. 522-529
22. Quamme H A, Van Der Zwet T & Dirks V (1976). Relation of fireblight resistance of young pear seedlings inoculated in the greenhouse to mature seedlings trees naturally infected in the field. Plant Disease Report 60: 660-664
23. Reynoird J P, Mourgues F, Chevreau E, Brisset M N & Aldwickle H S (1999a). Expression of SB-37 gene in transgenic pears enhanced resistance to fire blight. Acta Horticulturae 489: 243-245
24. Reynoird J P, Mourgues F, Chevreau E, Brisset M N & Aldwickle H S (1999b). First evidence for differences in fire blight resistance among transgenic pear clones expressing attacin E gene. Acta Horticulturae 489: 245-247
25. Rosati C, Rivalta L, Dradi M, Le Lézec M, Belouin A & Chartier R (2002). Fireblight evaluation of advanced Italian selections and cultivars of pear. Acta Horticulturae 596: 279-282
26. Ryugo K (1982). Breeding resistance to fire-blight bacteria, Erwinia amylovora, in pears. Acta Horticulturae 33-36
27. Saygılı H, Türküsay H, Hepaksoy S, Ünal A & Can H Z (1999). Investigation on determining some pear varieties resistant to fire blight (Erwinia amylovora (Burrill.) Winslow et al). Acta Horticulturae 489: 225-229
28. Saygılı H, Aysan Y, Mirik M & Şahin F (2004). Severe outbreak of fire blight on quince in Turkey. Acta Horticulturae 704: 51-53
29. Sestras A, Sestras R, Barbos A & Madalina M (2008). The differences among pear genotypes to fire blight (Erwinia amylovora) attack, based on observations of natural infection. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 36(2): 98-103
30. Shoemaker J C & Teskey B J E (1959). Tree Fruit Production. John Wiles & Sons, Inc. London, New York
31. Spotts R A & Mielke E A (1999). Resistance of pear cultivars in Oregon to natural fire blight infection. Fruit Varieties Journal 53(2): 110-115
32. Thibault B, Lecomp P, Hermann L & Belouin A (1987). Assesment of the susceptibility to Erwinia amylovora of the 90 varieties or selections of pear. Acta Horticulturae 217: 305-309
33. Thompson S S, Janick J & Williams E B (1962). Evaluation of resistance to fire blight of pear. Proceedings of the American Society for Horticultural Science 80: 105- 113
34. Ünal A, Saygılı H, Hepaksoy S, Türküsay H & Can Z (1998). Researches on determining pear varieties resistant to fire blight (Erwinia amylovora (Burril.) Winslow et al) in the Western Anatolia. TÜBİTAK, TOGTAG Project number 1247, Final Report
35. Van Der Zwet T & Beer S V (1995). Fire blight-It’s nature, prevention and control: A practical guide to integrated disease management. U.S. Department of Agriculture, Agriculture Information Bulletin No: 631
36. Yılmaz M A & Aysan Y (2009). Erwinia amylovora’nın neden olduğu ateş yanıklığı hastalığının elmalardan izolasyonu, belirtileri, yayılması ve mücadelesi. Tarım Bilimleri Araştırma Dergisi 2(1): 75-77
37. Yoder K S & Biggs A R (2010). Table of Pear Cultivar Susceptibility to the Fire Blight Bacterium. Davis College of Agriculture, Natural Resources and Design, Division of Plant and Soil Science. http://www.caf. wvu.edu/Kearneysville/tables/ pearfireblightsus.htm (Erişim tarihi: 16.09.2014)