Abstract
Bu araşt ı rman ı n amac ı değişik lüpin tür'erinden Lupinus spp protoplast izole etmek için uygun yöntem geliştirmektir. Al 10 ml r , CaCl2. 2H20 0.103 g I , Cellulase Onozuka R-10 1.5 %, Macerozyme R-10 0.1%, mannitol 8.96 % ve BSA 0.1% kar ışımı serada yetiştirilerblüpin türleri yapraklar ı ndan protoplast elde etmeye uygun bir enzim kar ışımı olarak belirlendi. Karanl ı kta, buzdolabı nda +4 C 14-b5 saat hücre duvar ı n ı enzimle uzakla ştı rma bunu takiben de oda s ı caklığı nda 30-60 dakika inkube etme ve daha sonra 25 C de 70 devir! dk. 30 dakika sallama ile ara ştı rmada kullan ı lan Bütün lüpin türlerinden yüksek kalite ve miktarda protoplast elde edildi.
Keywords
References
- Bhojwani, S.S.,and M.K. Razdan,1983. Plant tissue culture: Theory and Practice Davey, M.R and A. Kumar (1983), Higher plant protoplasts-Retrospect and prospect. Ir, intemational review of cytology, supplement 16. plant protoplasts ed. K.L. Giles pp:1-3
- Dupuis,J.M; M. Pean and Chagvardieff (1990), Plant donor tissue and isolation procedure efbct on early formation of embryoids from protoplasts of Hellanthus annuus L. Plant Cell, Tissue and Organ Culture 22: 183-189
- Fellner,M and P. Havranek (1992), Isolation of Album pollen protoplasts. Plant Cell, Tissue and Organ Culture 29: 275-279
- Fitterr, M.S., and A.D. Krikorian, 1989. Plant protoplasts some guidlines for their preparabon and manipulation in culture. Calbiochem Corporation.
- Gilbert, J.E.,1991. Interaction between mixing and density in protoplast cultures of Solanum tuberosum L. PhD thesis, University of Reading, Department of Agriculturai Botany.
- Larkin, P.j., 1976. Purification and viability determinations of plant protoplasts. Planta 128: 213-216
- Lee,N., H.Y. Wetzstein and C.H. Borman (1989) Cortical microtubule organization in Vibs protoplasts affected by concentration of enzyme isolation medium and duration of incubation. Physioloa plantamm 77: 27-32
- Sarhan, F., and D. Cesar, 1988. High yield isolation of mesophyll protoplasts from wheat, barley and rye. Physiologia Plantarum, 72: 337-342.
- Schafer-Menuhr, A., 1987. Isolation und kultur von lupinen protoplasten. I. Protoplasten aus blattern von Lupinus angustifolius sorte kubesa. Landbaufarschung volkenrode, 37(3): 117-120
- Schafer-Menuhr, A. (1988) Isolation und kultur vonlupinen protoplasten. III. Protoplasten aus zellsuspensionskulturen von Lupinus polyphyllus. Landbaufarschung volkenrode, 38. Heft 2, Seite 99-102
- Schafer-Menuhr, A.(1989) Isolation und kultur von lupinen protoplasten. IV. Regeneration von pflanzen aus protoplasten. Landbaufarschung volkenrode, 39. Heft 3, Seite 133-136
- Schafer-Menuhr, A (1990) Electrical fusion of lupin species. In, International lupin associallon 6th intemallonal lupin conference proceedings. Temuco-Pucan Chile. Edit. by D. von Barer pp: 373-375
- Schafer-Menuhr, A (1991) regeneration of shoots from protoplasts of lupins. Lupin newsletter. 14: 42-44
- Schafer-Menuhr, A.,and S. Sturmer, 1987. Isolation und kultur von lupinen protoplasten. Il. Modifikation von nahrmedien zur beschleunigten teilung von protoplasten aus blattern von Lupinus angustifolius sorte kubesa. Landbaufarschung volkenrode. 37(4) 231-234
- Vasil, V and I.K. Vasil (1980) Isolation and culture of cereal protoplats. Il Embryogenesis and plantlet formation from protoplasts of Pennisetum americanum. Theor Apll. Genet. 56: 97
Abstract
The aim of this wprk was to find out a method for isolation of+ protoplasts from various lupin Lupinus spp species. Al 10 ml I , CaCl2. 2H20 0.103 g I , Cellulase Onozuka R-10 1.5 %, Macerozyme R-10 0.1%, mannitol 8.96 % and BSA 0.1% have been found to be a suitable enzyme mixt yre for isolating protoplasts from the leaves of glasshouse-grown lupin species. Digestion in the dark in the refrigerator C for 14-15 hours followed by incubation at room temperature for 30- 60 minutes and then shaking at 70 rpm at 25 C for 30 minutes released a high quality and number of protoplasts in all lupin species examined.
Keywords
References
- Bhojwani, S.S.,and M.K. Razdan,1983. Plant tissue culture: Theory and Practice Davey, M.R and A. Kumar (1983), Higher plant protoplasts-Retrospect and prospect. Ir, intemational review of cytology, supplement 16. plant protoplasts ed. K.L. Giles pp:1-3
- Dupuis,J.M; M. Pean and Chagvardieff (1990), Plant donor tissue and isolation procedure efbct on early formation of embryoids from protoplasts of Hellanthus annuus L. Plant Cell, Tissue and Organ Culture 22: 183-189
- Fellner,M and P. Havranek (1992), Isolation of Album pollen protoplasts. Plant Cell, Tissue and Organ Culture 29: 275-279
- Fitterr, M.S., and A.D. Krikorian, 1989. Plant protoplasts some guidlines for their preparabon and manipulation in culture. Calbiochem Corporation.
- Gilbert, J.E.,1991. Interaction between mixing and density in protoplast cultures of Solanum tuberosum L. PhD thesis, University of Reading, Department of Agriculturai Botany.
- Larkin, P.j., 1976. Purification and viability determinations of plant protoplasts. Planta 128: 213-216
- Lee,N., H.Y. Wetzstein and C.H. Borman (1989) Cortical microtubule organization in Vibs protoplasts affected by concentration of enzyme isolation medium and duration of incubation. Physioloa plantamm 77: 27-32
- Sarhan, F., and D. Cesar, 1988. High yield isolation of mesophyll protoplasts from wheat, barley and rye. Physiologia Plantarum, 72: 337-342.
- Schafer-Menuhr, A., 1987. Isolation und kultur von lupinen protoplasten. I. Protoplasten aus blattern von Lupinus angustifolius sorte kubesa. Landbaufarschung volkenrode, 37(3): 117-120
- Schafer-Menuhr, A. (1988) Isolation und kultur vonlupinen protoplasten. III. Protoplasten aus zellsuspensionskulturen von Lupinus polyphyllus. Landbaufarschung volkenrode, 38. Heft 2, Seite 99-102
- Schafer-Menuhr, A.(1989) Isolation und kultur von lupinen protoplasten. IV. Regeneration von pflanzen aus protoplasten. Landbaufarschung volkenrode, 39. Heft 3, Seite 133-136
- Schafer-Menuhr, A (1990) Electrical fusion of lupin species. In, International lupin associallon 6th intemallonal lupin conference proceedings. Temuco-Pucan Chile. Edit. by D. von Barer pp: 373-375
- Schafer-Menuhr, A (1991) regeneration of shoots from protoplasts of lupins. Lupin newsletter. 14: 42-44
- Schafer-Menuhr, A.,and S. Sturmer, 1987. Isolation und kultur von lupinen protoplasten. Il. Modifikation von nahrmedien zur beschleunigten teilung von protoplasten aus blattern von Lupinus angustifolius sorte kubesa. Landbaufarschung volkenrode. 37(4) 231-234
- Vasil, V and I.K. Vasil (1980) Isolation and culture of cereal protoplats. Il Embryogenesis and plantlet formation from protoplasts of Pennisetum americanum. Theor Apll. Genet. 56: 97
Details
| Primary Language | English |
|---|---|
| Subjects | Engineering |
| Journal Section | Research Article |
| Authors | |
| Publication Date | May 1, 1996 |
| Submission Date | May 1, 1996 |
| Published in Issue | Year 1996 |
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