Uzun Yapraklı Üçgülün Trifolium pannonicum ssp. elongatum Hipokotil ve Yaprak Sapı Eksplantından in vitro Çoğaltılması

Abstract

Bu çalışmada, kinetin ve α- naftalin asetik asitin NAA ’in farklı konsantrasyonlarının kinetin ve 0.1+ 0.1, 0.2, 0.4 NAA mg/l , uzun yapraklı üçgül Trifolium pannonicum ssp. elongatum bitkisininhipokotil ve kotiledon yaprak sapı eksplantlarından gelişen sürgün yüzdesi, sürgün sayısı ve sürgün uzunluğu üzerine olan etkileri araştırılmıştır. Araştırmada, sürgün oluşturan eksplant yüzdesinin en yüksek oranda % 91.7 hipokotil eksplantından ve Murashige and Skoog MS içinde 1 mg/l Kinetin + 0.4 mg/l NAA içeren ortamdan; en düşük sürgün yüzdesinin % 25.0 hipokotil ve kotiledon yaprak sapı eksplantlarının her ikisinde de 1 mg/l Kinetin + 0.1 NAA içeren rejenerasyon ortamında geliştiği belirlenmiştir. Eksplant başına en fazla sürgün sayısı 11.2±1.3 adet ile 1 mg/l Kinetin+0.4 mg/l NAA içeren ortamdan, en düşük sürgün sayısı 2.5±0.6 adet ile 1 mg/l kinetin + 0.1 NAA uygulamasında gözlenmiştir. En uzun sürgün gelişimi 3.5±0.5 cm ile 1 mg/l kinetin+ 0,1 mg/L NAA içeren MS ortamında, en kısa sürgün gelişimi ise 1.5±0.2 cm ile 1 mg/l kinetin + 0.4 NAA MS ortamında belirlenmiştir. Trifolium pannonicum JACQ. ssp. elongatum bitkisinden maksimum kök gelişimi % 95±2.9 ile 1 mg/l IBA uygulamasından elde edilmiştir

Keywords

• Trifolium pannonicum ssp. elongatum
• hipokotil
• kotiledon yaprak sapı
• adventif sürgün rejenerasyonu
• köklenme

In vitro Propagation of Long Leaved Clover Trifolium pannonicum ssp. elongatum Using Hypocotyle and Petiole Explants

Abstract

The study reports effects of various concentrations of Kinetin and α- Naftalin asetik asit NAA kinetin and 0.1+ 0.1, 0.2, 0.4 NAA mg/l on shoot regeneration percentage, number and length of shoot of Trifolium pannonicum ssp. elongatum from hypocotyl and petiole of cotyledon leaf. The maximum 91.7% shoot regeneration frequency 25% was recorded on hypocotyl explant on Murashige and Skoog MS medium containing 1 mg/l Kinetin + 0.4 mg/l NAA; whereas, minimum shoot regeneration frequency 25% was in medium containing 1 mg/l Kinetin + 0.1 NAA respectively, in both hypcotyl and petiole of cotyledon leaf explant. The highest number of 11.2±1.3 shoots per explant was recorded on MS medium containing 1 mg/l Kinetin+0.4 mg/l NAA and the lowest number of 2.5±0.6 was in medium containing 1 mg/l Kinetin+0.1 mg/l NAA respectively. The longest shoots 3.5±0.5 cm were recorded on MS medium containing 1 mg/l kinetin+ 0,1 mg/L NAA, and the smallest shoots 1.5±0.2 cm were recorded on MS medium containing 1 mg/l kinetin + 0.4 NAA. Maximum frequency of 95% roots was recorded on MS medium containing 1 mg/l IBA

Keywords

• Trifolium pannonicum ssp.elongatum
• hypocotyl
• petiole of cotyledon
• adventitious shoot regeneration
• rooting

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