Farklı Sulandırıcı ve Saklama Sürelerinin Üreme Mevsimi Boyunca Ot Sazanı Ctenopharyngodon idella Spermasının Motilite ve Fertilizazyon Başarısı Üzerine Etkisi

Abstract

Bu araştırma, üreme mevsimi boyunca kısa süreli saklama işlemi uygulanan ot sazanı spermasında farklı sulandırıcı ve saklama sürelerinin motilite ve fertilizasyon yeteneği üzerine etkisini belirlemek amacıyla yürütülmüştür. Sperma örneği, anestezi uygulanan erkek anaç balıklardan abdominal masaj yoluyla alınmıştır. Başlıca spermatolojik özelliklerin belirlenmesinin ardından aynı kapta toplanan spermler 3 farklı sulandırıcı ile 1:3 oranında dilüe edilmiştir. Dilüe edilen spermler küçük tüplere aktarılmış ve buzdolabında +4°C’de 8 saat süre ile muhafaza edilmiştir. Muhafaza süresince spermanın motilite % , motilite süresi s ve fertilizasyon % kapasitesi 2’şer saat arayla incelenmiştir. Fertilizasyon işlemi kuru fertilizasyon tekniğine göre 2x105spermatozoa/yumurta dölleme dozunda yapılmıştır. Döllenme başarısı fertilizasyonu takiben 3-4 gün sonra göz lekeli yumurtaların yüzdesine göre belirlenmiştir. 8 saatlik muhafaza süresinin sonunda en yüksek motilite II no’lu sulandırıcı ile 35±2.89% oranında P

Keywords

• sperma kalitesi
• sulandırıcı
• soğuk muhafaza
• fertilizasyon
• ot sazanı

Effect of Different Extenders and Storage Periods on Motility and Fertilization Success of Grass Carp Ctenopharyngodon idella Sperm During Spawning Season

Abstract

The present study was conducted to evaluate the effect of different extenders and storage periods on motility and fertilizing ability of short-term stored grass carp sperm during spawning season. Sperm was collected from anesthesized males by the abdominal massage method. Having determined the main spermatological properties, the pooled ejaculates were diluted with 3 different extenders at a ratio of 1:3. The diluted sperm was packaged in small tubes and following, the tubes were stored at 4°C in a refrigerator for 8 hours. During preservation motility % , motility duration s and fertilization % capacity of stored sperm were evaluated 2 hours intervals. Fertilization was carried out using the dry fertilization technique at 2x105spermatozoa/egg dosage. The fertilization success was determined as the percent of eyed eggs 3-4 days after fertilization. At the end of 8 hours of storage, the highest sperm motility was found as 35±2.89% P

Keywords

• sperm quality
• extender
• cold storage
• fertilization
• grass carp

References

1. Aas, G.H., T. Refstie and B. Gjerde. 1991. Evaluation of milt quality of atlantic salmon. Aquaculture 95: 125-132.
2. Akcay, E., Y. Bozkurt, S. Secer and N. Tekin. 2004. Cryopreservation of mirror carp semen. Turkish Journal of Veterinary and Animal Sciences 28 (5): 837-843.
3. Belova, N.V. 1981. The ecologial and physiological pecularities of sperm in pond cyprinids. Journal of Ichthyology 21: 90-102.
4. Benau, D. and C. Terner. 1980. Initiation, prolongation and reactivation of the motility of salmonid spermatozoa. Gamete Research 3: 247-257.
5. Billard, R. 1981. Short-term preservation of sperm under oxygen atmosphere in rainbow trout (Salmo gairdneri). Aquaculture 23(1-4):287-293.
6. Billard, R., J. Cosson, L.W. Crim and M. Suquet. 1995. Sperm Physiology and Quality. In: Broodstock Management and Egg and Larval Quality. Blackwell Science, Oxford, pp: 25-52.
7. Bozkurt, Y. and S. Seçer. 2005. Effect of short-term preservation of mirror carp (Cyprinus carpio) semen on motility, fertilization and hatching rates. The Israeli Journal of Aquaculture-Bamidgeh 57(3):207-212.
8. Bozkurt, Y., F. Öğretmen, U. Erçin, and Ü. Yιldιz. 2008. Seminal plasma composition and its relationship with physical spermatological parameters of grass carp (Ctenopharyngodon idella) semen: with emphasis on sperm motility. Aquaculture Research 39:1666-1672.

9. Buyukhatipoglu, S. and W. Holtz. 1978. Preservation of trout sperm in liquid or frozen state. Aquaculture 14:49-56.
10. Carpentier, M. and R. Billard. 1978. Conservation a court terme des gametes de salmonides a des temperatures voisines de 0°C. Annales de Biologie Animale Biochimie Biophysique 18:1083-1088.
11. Chereguini, O., R.M. Cal, C. Dreanno, B.O. deBaulny, M. Suquet and G. Maisse. 1997. Short-term storage and cryopreservation of turbot (Scophthalmus maximus) sperm. Aquatic Living Resources10 (4) 251-255.
12. Cloud, J.G., W.H. Miller and M.J. Levanduski. 1990. Cryopreservation of sperm as a means to store salmonid germ plasm and to transfer genes from wild fish to hatchery populations. Progresssive Fish Culturist 52(1):51-53.
13. Harvey, B. and R.N. Kelley. 1984. Chilled storage of Sarotherodon mossambicus milt. Aquaculture 36:85-95.
14. Hulata, G. and S. Rothbard. 1979. Cold storage of carp semen for short periods. Aquaculture 16:267-269.
15. Iwamatsu, T., S. Ishijima and S. Nakashima. 1993. Movement of spermatozoa and changes in micropyles during fertilization in medaka eggs. Journal of Experimental Zoology 266 (1):57-64.
16. Leung, L.K.B. and B.G.M. Jamieson. 1991. Live preservation of fish gametes. In: Jamieson, B.G.M. (Ed.), Fish Evolution Spermatozoa. Cambridge Univ. Pess, Cambridge, pp. 245-269. Evidence from
17. Lahnsteiner, F. and R.A. Patzner. 1998. Sperm motility of the marine teleosts Boops boops, Diplodus sargus, Mullus barbatus and Trachurus mediterraneus. Journal of Fish Biology 52(4):726-742.
18. Lahnsteiner, F.,T. Weismann and R.A. Patzner. 1998. An efficient method for cryopreservation of testicular sperm of the Northern pike, Esox lucius Linnaeus. Aquaculture Research 29:341-347.
19. Lubzens, E., N. Daube, I. Pekarsky, Y. Magnus, A. Cohen, F. Yusefovich and P. Feigin. 1997. Carp (Cyprinus carpio L.) spermatozoa cryobanks - strategies in research and application. Aquaculture 155:13-30.
20. Ritar, A.J. 1999. Artificial insemination with cryopreserved semen from striped trumpeter (Latris lineata). Aquaculture 180:177-187.
21. Ritar, A.J. and M. Campet. 2000. Sperm survival during short- term storage and after cryopreservation of semen from striped trumpeter (Latris lineata). Theriogenology 54 (3): 467-480.
22. Saad, A., R. Billard, M.C. Theron and M.G. Hollobeeq. 1988. Short-term preservation of carp (Cyprinus carpio) semen. Aquaculture 71:133-150.
23. Scott, A.P. and S.M. Baynes. 1980. A review of the biology, handling and storage of salmonid spermatozoa. Journal of Fish Biology 17: 07-739.
24. Stoss, J. and W. Holtz. 1983. Succesful storage of chilled rainbow trout (Salmo gairdneri) spermatozoa for up to 34 days. Aquaculture 31: 269-274.
25. Stoss, J. and T. Refstie. 1983. Short-therm storage and cryopreservation of milt from atlantic salmon and sea trout. Aquaculture 30:229-236.
26. Sunitha, M.S. and V. Jayaprakas. 1997. Influence of pH, temperature, salinity and media on activation of motility and short term preservation of spermatozoa of an estuarine fish, Mystus gulio (Hamilton) (Siluridae- Pisces). Indian Journal of Marine Sciences 26(4):361- 365.
27. Tekin, N., S. Secer, E. Akcay and Y. Bozkurt. 2003. Cryopreservation of rainbow trout (Oncorhynchus mykiss) semen, The Israeli Journal of Aquaculture- Bamidgeh 55(3): 208-212.
28. Terner, C. 1986. Evaluation of salmonid sperm motility for cryopreservation. The Progressive Fish Culturist 48: 230-232.
29. Truscott, B., D.R.VIdler, R.J. Hoyle, and H.C. Freeman. 1968. Sub-zero preservation of Atlantic salmon sperm. Journal of the Fisheries Research Board of Canada 25: 363-372.
30. Yao, Z., G.F. Richardson and L.W. Crim. 1999. A diluent for prolonged motility of ocean pout (Macrozoarces americanus L.) sperm. Aquaculture 174 (1-2) 183-193.
31. Zhukinskiy, V.N. and V.R. Alekseenko. 1983. Semen quality in common carp, Cyprinus carpio and White Amur, Ctenopharyngodon idella (Cyprinidae), in different periods of the spawning season and as influenced by extraction methods. Journal of Ichthyology 23:124-133.