Determination of Amitraz Varroaset Residue in Honey by High Performance Liquid Chromatography HPLC

Abstract

Amitraz Varroaset is an acaricide applied against Varroa destructor Anderson & Trueman Acarina: Varroidae infestations on honeybees Apis mellifera L. Hymenoptera: Apidae . Amitraz residue in honey was determined by HPLC in Ankara, Turkey. Honey samples were collected from beekeepers during the harvesting period in Ayaş, Kızılcahamam and Polatlı towns in Ankara. Amitraz residue was found in various levels in six 15% out of 32 honey samples. The residue amounts in the 6 samples were 5.35, 0.34, 0.23, 1.27, 0.92 and 0.40 ppm. The limit of detection was 0.0643 ppm and the recovery ratio was 56.71 %. According to the results, some honey samples showed higher residue levels of amitraz than the World Health Organisation WHO limit of 1 mg/kg

Keywords

• Amitraz,residue,HPLC,honey,Varroa destructor,Apis mellifera

Ballarda Amitraz Varroaset Kalıntısının Yüksek Performans Likit Kromatografi Yöntemi HPLC ile Belirlenmesi

Öz

Amitraz Varroaset bal arılarında Apis mellifera L. Hymenoptera: Apidae sorun olan Varroa destructor Anderson & Trueman Acarina: Varroidae „e karşı kullanılan bir akarisittir. Ballardaki amitraz kalıntısı HPLC ile tespit edilmiştir. Bal örnekleri arı yetiştiricilerinden Ankara‟nın ilçeleri olan Ayaş, Kızılcahamam ve Polatlı‟dan hasat dönemi boyunca toplanmıştır. Toplanan 32 örnekten 6 %15 adedinde amitraz kalıntısına rastlanılmıştır. Örneklerde tespit edilen kalıntı miktarları sırasıyla 5.35, 0.34, 0.23, 1.27, 0.92 ve 0.40 ppm. dir.Tespit edilen kalıntı limiti 0.0643 ppm ve elde edilme oranı %56,71 dir. Bu sonuç doğrultusunda Dünya Sağlık Örgütü WHO ‟ nün izin vermiş olduğu limit 1 mg/kg iken bazı bal örneklerinde daha yüksek düzeyde amitraz kalıntısı olduğu görülmektedir

Anahtar Kelimeler

• Amitraz,kalıntı,HPLC,bal,Varroa destructor,Apis mellifera

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