Tumor Formation in Isatis constricta Davis woad Using Oncogenic A281 Strain of Agrobacterium tumefaciens

Abstract

Leaf and petiole explants obtained from in vitro grown seedlings of Isatis constricta woad were treated with oncogenic A281 strain of Agrobacterium tumefaciens. Tumour formation started on explants after 15 days and continued until 4 weeks when gus test was performed. The highest gus positive results were recorded from petiole explants selected on medium containing 50 mg/l kanamycine under 16 h light photoperiod

Keywords

• Isatis constricta woad,Agrobacterium tumefaciens,GUS analysis,tumour formation

Onkogenik Agrobacterium tumefaciens A281 hattı ile çivit otu Isatis constricta Davis bitkisinde tümör oluşumu

Abstract

Bu çalışmada, Isatis constricta çivit otu bitkisinden çimlendirme sonucu elde edilen in vitro gelişen yaprak ve yaprak sapı eksplantları Agrobacterium tumefaciens’in onkogenik A281 hattıyla inokule edilmiştir. Tümör oluşumu, eksplantlar inoküle edildikten 15 gün sonra başlamış olup, 4 hafta sonra GUS analizi yapılmıştır. En fazla tümöt oluşumu yaprak sapı eksplantında 50 mg/l kanamisin içeren aydınlık 16 saat ışık fotoperiyodunda denemesinden elde edilmiştir

Keywords

• Isatis constricta çivit otu,Agrobacterium tumefaciens,GUS analizi,tümör oluşumu

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