Urease Activity and its Kinetics in Soil Treated with Tobacco Waste and Wheat Straw

Abstract

This research was conducted to determine the effects of tobacco waste and straw incorporated to soil on urease activity and kinetics by an incubation experiment. For this purpose, clay loam soil sample and organic wastes were mixed until reaching 5% dry weight and this mixture was incubated in 25±0.5°C temperature for 30 days. Following incubation period, urease activity and changing of kinetic parameters were searched in soil samples in different substrate concentrations 0,1,2,4,6,8,10 and 12% , incubation periods 0,1,2,3,4,5 and 6 hours and incubation temperatures 0,10,20,30,40 and 50°C . Research results showed that treatments of tobacco waste and wheat straw increased urease activity in soil. It was also determined that reaction velocity increased until the level of 8% substrate concentration for control and 10% for tobacco waste and wheat straw treatments. Then it did not change for both of them when exceeding these thresholds. The highest reaction velocity in all substrate concentrations was observed in 50°C incubation temperature for all soil samples including control. In all treatments, the highest Vmax and Km values were measured between 40-50°C. In addition, the highest Vmax/Km ratio for control and soil treated with organic wastes were observed in 50°C and 40°C, respectively

Keywords

• Soil
• enzyme
• urease
• kinetic parameters
• wheat straw
• tobacco waste

Tütün Atığı ve Buğday Samanı Uygulanmış Toprakta Üreaz Aktivitesi ve Kinetiği

Abstract

Bu araştırmada, topraklara %5 düzeyinde uygulanan tütün atığı ve buğday samanının üreaz aktivitesi ve kinetiği üzerine etkisi bir inkübasyon denemesi ile saptanmıştır. Bu amaçla, killi tın bünyeli deneme toprağına organik atıklar, kuru ağırlık üzerinden %5 oranında karıştırılmış ve 30 gün süre ile 25±0.5 C’de inkübasyona bırakılmıştır. İnkübasyon sonunda alınan örneklerin, farklı substrat konsantrasyonları %0, %1, %2, %4, %6, %8, %10 ve %12 , inkübasyon periyotları 0, 1, 2, 3, 4, 5 ve 6 saat ve sıcaklıklarındaki 0, 10, 20, 30, 40 ve 0C üreaz aktivitesi ve kinetik parametrelerindeki değişimler araştırılmıştır. Elde edilen sonuçlara göre, topraklara tütün atığı ve buğday samanı uygulamasının üreaz aktivitesini artırdığı belirlenmiştir. Ayrıca, reaksiyon hızının kontrolde %8’lik substrat düzeyine, tütün atığı ve buğday samanı uygulamasında ise %10’luk düzeyine kadar artış gösterdiği ve bu düzeylerden sonra değişmediği belirlenmiştir. Hem kontrol hem de organik atık uygulanmış topraklarda tüm substrat konsantrasyonlarındaki en yüksek reaksiyon hızları 500C’lik inkübasyon sıcaklığında saptanmıştır. Tüm uygulamalarda en yüksek Vmax ve Km değerleri 40 ve 500C’de, en yüksek Vmax/Km oranları kontrol uygulamasında 50 0C’de, tütün atığı ve buğday samanı uygulamasında ise 0C’de belirlenmiştir

Keywords

• Toprak
• enzim
• üreaz
• kinetik parametreler
• buğday samanı
• tütün atığı

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