Tumor Formation in Sunflower Using A281 Strain of Agrobacterium tumefaciens

Abstract

One week old in vitro plantlets of nine lines selected from genotype Ekiz of sunflower were used in this study. Leaf, petiole and immature cotyledon explants from each line were treated with oncogenic A281 strain of Agrobacterium tumefaciens. Tumors induction started on all explants with in 6-7 days of culture and 100% tumor formation was observed on all explants after 4 weeks of culture

Keywords

• Helainthus annuus L. Agrobacterium tumefaciens,oncogenic,tumor formation

Yabani Agrobacterium tumefaciens A281 Hattıyla Ayçiçeğinde Tümör Oluşumu

Öz

Bu çalışmada ayçiçeğinin Ekiz genotipinden seçilen 9 farklı hatta ait in vitro gelişen bir haftalık bitkicikler kullanılmıştır. Her hattan yaprak, yaprak sapı ve olgunlaşmış kotiledon eksplanları bitkiciklerden izole edilerek Agrobacterium tumefaciens’in onkogenik A281 hattıyla inokule edilmişlerdir. Tümör oluşumu eksplantlar inoküle edildikten 6-7 gün sonra başlamış olup, 4 hafta sonra denen ayçiçeği hatlarına ait bütün eksplantlarda tümör oluşumu gözlenmiştir

Anahtar Kelimeler

• Helianthus annuus L.,Agrobacterium tumefaciens,onkogenik,tümör oluşumu

References

1. Alibert, B., O. Lucas, V. Gall and J. Kallerhoff, (1999). Pectolytic enzyme treatment of sunflower prior to wounding and cocultivation with Agrobacterium tumefaciens, enhances efficieny of transient beta glucuronidase expression, Phyiologia Plantarum 106:2, 232-237.
2. Bidney, D., C. Scelonge, J. Martich, M. Burrus, L. Sims and G. Hoffman, 1992. Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens. Plant Mol. Biol., 18: 301- 313.
3. Escandon, A.S. and G. Hahne, 1991. Genotype and composition of culture medium are factors important for transformed sunflower (Helianthus annuus L) callus.Physiol. Plant., 81: 367-376.
4. Pugliesi, C., P. Megale, F. Cecconi and S. Baroncelli, 1993.
5. Evrett, N. P., K.E.P. Robinson and D. Mascarenhas, 1987. Genetic Engineering of Sunflower (Helianthus annuus), Bio/Technology, 5; 1201-1204 .
6. Gaponenko, AK., IP.Voronina, Yu, D. Beletskii, AS. Konov and K.Skryabin, 1991.Effectiveness of the transformation of immature embriyos of sunflower (Helianthus annuus L.)1 Vsesoyuznyi simpozium Novye metody bioteknologii rastenii, Pushcino,123: 13-14.
7. Grayburn, W.S. and B.A. Vick, 1995. Transformation of sunflower (Helianthus annuus L.) following wounding with glass beads. Plant Cell Rep., 14: 285-289. The hyper virulence of Agrobacterium tumefaciens A281 is encoded in a region of p Ti Bo542 outside of T- DNA.’’ J. Bacteriol., 168; 1291-1301.
8. Jefferson, R.A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5; 387- 405.

9. Knittel, N., V. Gruger, G. Hahne and P. Lénéé, 1994. Transformation of sunflower (Helianthus annuus L.): A reliable protocol. Plant Cell Rep., 14: 81-86.
10. Laparra, H., M. Burrus, R. Huonold, B. Damm, A.M. Bravo- Angel, R. Bronner and G. Hahne, 1995. Expression of foreign genes in sunflower (Helianthus annuus) L.) evaluation of three gene transfer methods. Euphytica, 85: 63-74.
11. Moyne, A.L., D. Tagu, V. Thor, C. Bergounioux, G. Freyssinet and P. Gadal, 1989.Transformation calli obtained by direct gene transfer into sunflower protoplast. Plant Cell Rep., 8: 97-100.
12. Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum.15; 473-497.
13. Özcan, S. 1993. Tissue culture in pea and engineering a marker gene for specific expression in target cells for plant transformation. (Ph.D. Thesis), University of Leicester, U.K. Organogenesis and embryogenesis İN Helianthus tuberosus and in the interspecific hybrid Helianthus annuus x H. tuberosus. Plant Cell Tiss. Org. Cult., 33: 187-193.
14. Schrammeijer, B., P.C. Sijmons, P.J.M. van den Elzen and A. Hoekema, 1990. Meristem transformation of sunflower via Agrobacterium. Plant Cell rep. 9: 55-60